Pilot scale production of a heterologous Trichoderma reesei cellulase by Saccharomyces cerevisiae

Zurbriggen, Beat; Bailey, Michael; Penttilä, Merja; Poutanen, Kaisa; Linko, Matti

doi:10.1016/0168-1656(90)90075-m

Cited by 58 publications

(20 citation statements)

References 13 publications

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“…Several cellulase genes from various fungi have also been expressed in S. cerevisiae. Five endo-␤-1,4-glucanases (encoded by egl1, egl2, egl3, egl4, and egl5) and two cellobiohydrolases (CBHI and CBHII) from T. reesei were efficiently secreted into the culture medium by S. cerevisiae transformants (4,27,521,522,525,586,588,655,783,784). Similar results were observed when the endo-␤-1,4-glucanase gene from Trichoderma longibrachiatum was expressed in a wine yeast strain (528,529).…”

Section: Heterologous Cellulase Expression In Bacteriasupporting

confidence: 70%

Microbial Cellulose Utilization: Fundamentals and Biotechnology

Lynd

Weimer

Zyl³

et al. 2002

Microbiol Mol Biol Rev

4,009

2,868

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Fundamental features of microbial cellulose utilization are examined at successively higher levels of aggregation encompassing the structure and composition of cellulosic biomass, taxonomic diversity, cellulase enzyme systems, molecular biology of cellulase enzymes, physiology of cellulolytic microorganisms, ecological aspects of cellulase-degrading communities, and rate-limiting factors in nature. The methodological basis for studying microbial cellulose utilization is considered relative to quantification of cells and enzymes in the presence of solid substrates as well as apparatus and analysis for cellulose-grown continuous cultures. Quantitative description of cellulose hydrolysis is addressed with respect to adsorption of cellulase enzymes, rates of enzymatic hydrolysis, bioenergetics of microbial cellulose utilization, kinetics of microbial cellulose utilization, and contrasting features compared to soluble substrate kinetics. A biological perspective on processing cellulosic biomass is presented, including features of pretreated substrates and alternative process configurations. Organism development is considered for “consolidated bioprocessing” (CBP), in which the production of cellulolytic enzymes, hydrolysis of biomass, and fermentation of resulting sugars to desired products occur in one step. Two organism development strategies for CBP are examined: (i) improve product yield and tolerance in microorganisms able to utilize cellulose, or (ii) express a heterologous system for cellulose hydrolysis and utilization in microorganisms that exhibit high product yield and tolerance. A concluding discussion identifies unresolved issues pertaining to microbial cellulose utilization, suggests approaches by which such issues might be resolved, and contrasts a microbially oriented cellulose hydrolysis paradigm to the more conventional enzymatically oriented paradigm in both fundamental and applied contexts

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Section: Heterologous Cellulase Expression In Bacteriasupporting

confidence: 70%

Microbial Cellulose Utilization: Fundamentals and Biotechnology

Lynd

Weimer

Zyl³

et al. 2002

Microbiol Mol Biol Rev

4,009

2,868

View full text Add to dashboard Cite

show abstract

“…-glucanase was assayed in a similar way but by using 1.0 g/100g barley -glucan (Biocon, Ireland) as substrate (17). Enzyme activity was expressed in katals (1 kat = 1 mol/s).…”

Section: Activitymentioning

confidence: 99%

Effects of Purified Endo-β-xylanase and Endo-β-glucanase on the Structural and Baking Characteristics of Rye Doughs

Autio¹,

Härkönen²,

Parkkonen³

et al. 1996

LWT - Food Science and Technology

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“…High cell density suspensions of the recombinant strains fermented amorphous cellulose, raw starch, and birchwood xylan to ethanol with yields of 0.45, 0.44 and 0.3 g ethanol/g substrate, respectively. Several cellobiohydrolases have been functionally expressed in S. cerevisiae (1,19,48,61,77). The specific activity of recombinant cellobiohydrolase was shown to be comparable to that of the native enzyme (61).…”

Section: Consolidated Bioprocessing (Cbp)mentioning

confidence: 95%