Pilot-Scale Membrane Filtration Process for the Recovery of an Extracellular Bacterial Protease

Separation of the protease enzymes ofBacillus licheniformis from the fermentation medium by crossflow ultrafiltration

“…12,23 Similar results have been reported by Bhodziewicz 14 and Pradanos and Hernandez. 17 The shearing effect of the cross¯owing solution limits the thickness of the cake formed by removing deposited particles from the membrane surface.…”

Comparison of different options for harvest of a therapeutic protein product from high cell density yeast fermentation broth

“…Under permeate control mode, flux is maintained constant, and the TMP is allowed to vary accordingly (Sheehan et al, 1988). An examination of Equation 8 suggests that an increase in TMP indicates higher resistance generated during microfiltration due to higher cake resistance (higher R c ), membrane pore plugging (higher R f ) or a combination of both mechanisms.…”

“…Bailey and Meagher (1997) performed a similar comparison between the hollow fiber and plate and frame formats for microfiltration of recombinant E. coli lysates and found both options to be comparable in performance under optimized conditions. Sheehan et al (1988) compared centrifugation with membrane based separations of extracellular bacterial protease and found the membrane process to be twice as cost effective as centrifugation and equivalent to a precoat filter, on basis of unit cost of enzyme product recovered. Industrial studies demonstrating robust operation of tangential flow filtration (TFF) based harvest of mammalian cell culture (van Reis et al, 1991) and CFF based harvest of recombinant yeast product have also been reported.…”

“…It has been observed that severe and often permanent fouling that occurs with operation under constant TMP due to very high initial permeate fluxes can be avoided by operating under constant flux (van Reis and Zydney, 2001). Sheehan et al (1988) observed an average flux increase of 2.5 times and protein transmission of 90% upon using permeate flow control for recovery of an extracellular protease. Harvest of mammalian cell culture using constant permeate flux at industrial scale has been shown to provide an average yield of 99% with the total cell number and viability maintained throughout the process (van Reis et al, 1991).…”

Development of continuous chitinase production process in a membrane bioreactor by Paenibacillus sp. CHE-N1

“…However, these operations are labor-intensive, time-consuming and energy consuming. Membrane technology provides another option for fermentation applications [18][19][20] in the clarification of broth [21][22][23]. The ceramic membranes with proper mechanical, thermal, and chemical properties, and being resistant to the harsh cleaning treatments, are considered a promising tool for developing into microfiltration (MF) process for the treatment of fermentation broth [24,25].…”