Pilot-scale isolation and characterization of extracellular polymeric substances (EPS) from cell-free medium of Spirulina sp. LEB-18 cultures under outdoor conditions

Jesus, Cristiane Santos de; Assis, Denílson de Jesus; Rodriguez, Manuela Bastos; Menezes-Filho, José A.; Costa, Jorge Alberto Vieira; Ferreira, Ederlan de Souza; Druzian, Janice Izabel

doi:10.1016/j.ijbiomac.2018.12.016

Cited by 36 publications

(11 citation statements)

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“…Applying outdoor cultivation systems can potentially reduce production costs by reducing light and temperature control [62,[90][91][92]. Relevance was found in an outdoor pilot scale cultivation of Spirulina sp.…”

Section: Process Conditionsmentioning

confidence: 99%

“…Then, an alcoholic (isopropanol or ethanol (2-3 v/v)) precipitation of polysaccharides is applied. Finally polysaccharides are freeze-or oven-dried [10,14,90,105]. Sometimes, the lipids and pigments of the biomass can be previously extracted using an organic solvent such as hexane, Even if these polysaccharides do not have covalent linkages with the cyanobacteria cell wall, they interact strongly with it, which requires specific processes to dissociate them.…”

Section: Downstream Processes and Cyano-eps Global Marketmentioning

confidence: 99%

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Exopolysaccharides from Cyanobacteria: Strategies for Bioprocess Development

et al. 2020

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Cyanobacteria have the potential to become an industrially sustainable source of functional biopolymers. Their exopolysaccharides (EPS) harbor chemical complexity, which predicts bioactive potential. Although some are reported to excrete conspicuous amounts of polysaccharides, others are still to be discovered. The production of this strain-specific trait can promote carbon neutrality while its intrinsic location can potentially reduce downstream processing costs. To develop an EPS cyanobacterial bioprocess (Cyano-EPS) three steps were explored: the selection of the cyanobacterial host; optimization of production parameters; downstream processing. Studying the production parameters allow us to understand and optimize their response in terms of growth and EPS production though many times it was found divergent. Although the extraction of EPS can be achieved with a certain degree of simplicity, the purification and isolation steps demand experience. In this review, we gathered relevant research on EPS with a focus on bioprocess development. Challenges and strategies to overcome possible drawbacks are highlighted.

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Section: Process Conditionsmentioning

confidence: 99%

Section: Downstream Processes and Cyano-eps Global Marketmentioning

confidence: 99%

Exopolysaccharides from Cyanobacteria: Strategies for Bioprocess Development

et al. 2020

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“…Exopolysaccharides can be recovered from the culture medium, resulting in the valorization of the process. Recently, studies have suggested the application of microalgal polysaccharides in nutraceuticals, food development, and as a bioflocculant [5,10,18,19] (Figure 1).…”

Section: Polysaccharides From Microalgaementioning

confidence: 99%

“…In the same study, Spirulina platensis presented predominantly rhamnose and glucose monosaccharides, in addition to the presence of glucuronic acid, mannose, glucosamine, fucose, and xylose. Jesus et al [18] reported glucose as the predominant monosaccharide in the composition of extracellular polymeric substances from Spirulina. Uronic acids, pyruvates, and acyl groups of carbohydrates or carboxylic groups of amino acids in the protein portion contribute to the anionic nature of exopolysaccharides [18,64].…”

Section: Chemical Compositionmentioning

confidence: 99%

Microalgae Polysaccharides: An Overview of Production, Characterization, and Potential Applications

et al. 2021

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Microalgae and cyanobacteria are photosynthetic microorganisms capable of synthesizing several biocompounds, including polysaccharides with antioxidant, antibacterial, and antiviral properties. At the same time that the accumulation of biomolecules occurs, microalgae can use wastewater and gaseous effluents for their growth, mitigating these pollutants. The increase in the production of polysaccharides by microalgae can be achieved mainly through nutritional limitations, stressful conditions, and/or adverse conditions. These compounds are of commercial interest due to their biological and rheological properties, which allow their application in various sectors, such as pharmaceuticals and foods. Thus, to increase the productivity and competitiveness of microalgal polysaccharides with commercial hydrocolloids, the cultivation parameters and extraction/purification processes have been optimized. In this context, this review addresses an overview of the production, characterization, and potential applications of polysaccharides obtained by microalgae and cyanobacteria. Moreover, the main opportunities and challenges in relation to obtaining these compounds are highlighted.

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“…The polysaccharide content in EPS was higher than the protein content, indicating polysaccharides were the main composition in EPS. Meanwhile, the presence of Zn(II) and Se(IV) increased the content of polysaccharide and protein, which formed the more stabled EPS [58]. More stabled EPS supplied many active sites to absorbed more m-dichlorobenzene molecules, so that m-dichlorobenzene could be effectively purified.…”

Section: Changes In Eps Chemical Compositionmentioning

confidence: 99%