UV disinfection is cost-effective and easy to maintain for decentralized areas. However, to ensure its effectiveness, some parameters need to be considered. In this study, a general search of Web of Science articles was performed to determine the possible influence of these parameters on the reactivation of microorganisms in UV systems; in addition, different search strings were used focusing exclusively on wastewater treatment, UV systems and Advanced Oxidation Processes (AOPs). It was found that in order to maintain low transmittance, it is essential to remove suspended solids and reduce water hardness. It is recommended to control the zeta potential in the range of 0–5 mV to avoid the aggregation of particles and bacteria. Determining the appropriate UV dose is essential to mitigate the reactivation of microorganisms. A minimum dose of 40 mJ/cm2 can contribute to effective disinfection and reduce the likelihood of reactivation. In addition, maintaining a residual chlorine level of at least 0.5 mg/L provides an additional barrier to reactivation. It is also important to optimize the design flow rate of the UV system as recommended for each individual unit. These measures, together with the combination of UV disinfection and chemical or AOPS, can effectively reduce the reactivation.