Pilot investigation of the molecular discrimination of human osteoblasts from different bone entities

Wein, Martin; Fretwurst, Tobias; Nahles, Susanne; Duttenhoefer, Fabian; Tomakidi, Pascal; Steinberg, Thorsten; Nelson, Katja

doi:10.1016/j.jcms.2015.07.030

Cited by 11 publications

(22 citation statements)

References 37 publications

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“…While mineralisation of hydrogels containing primary osteoblasts as well as osteoblast-like cell lines has been reported as early as after 7 days 53 , 54 , Wein et al . demonstrated a lower mineralisation capacity of osteoblasts originating from alveolar bone compared to iliac crest-derived bone from the same donor 55 . Hence, prolonged incubation times could enable advanced calcification of the printed jawbone models presented here 28 .…”

Section: Discussionmentioning

confidence: 92%

“…summarised several studies concluding that there is indeed a physiological difference between osteogenic cells from jawbone versus long bones, making them mandatory for generation of a jawbone model 61 . In line with this, several jawbone models have been proposed based on the origin of the isolated osteoblasts 23 , 25 , 27 , 55 , 62 .…”

Section: Discussionmentioning

confidence: 98%

“…Primary jawbone-derived human osteoblasts (JHOBs) were isolated following slightly modified protocols 36 , 55 . Briefly, small bone pieces (approximately 8 mm 3 ) of human mandibular jawbone were obtained from healthy donors during surgery (as approved by Charité’s Ethics Committee, EA1/038/19; informed consent of all participating subjects was obtained and all methods were performed in accordance with the relevant guidelines and regulations) and stored in growth medium [high-glucose DMEM supplemented with 10% fetal calf serum, 100 IE mL −1 penicillin, 100 µg mL −1 streptomycin and 2 mM GlutaMAX (Gibco, Thermo Fisher Scientific, Waltham, USA)] at 4 °C until isolation.…”

Section: Methodsmentioning

confidence: 99%

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3D bioprinting of tissue-specific osteoblasts and endothelial cells to model the human jawbone

Amler

Thomas

Tüzüner

et al. 2021

Sci Rep

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Jawbone differs from other bones in many aspects, including its developmental origin and the occurrence of jawbone-specific diseases like MRONJ (medication-related osteonecrosis of the jaw). Although there is a strong need, adequate in vitro models of this unique environment are sparse to date. While previous approaches are reliant e.g. on scaffolds or spheroid culture, 3D bioprinting enables free-form fabrication of complex living tissue structures. In the present work, production of human jawbone models was realised via projection-based stereolithography. Constructs were bioprinted containing primary jawbone-derived osteoblasts and vasculature-like channel structures optionally harbouring primary endothelial cells. After 28 days of cultivation in growth medium or osteogenic medium, expression of cell type-specific markers was confirmed on both the RNA and protein level, while prints maintained their overall structure. Survival of endothelial cells in the printed channels, co-cultured with osteoblasts in medium without supplementation of endothelial growth factors, was demonstrated. Constructs showed not only mineralisation, being one of the characteristics of osteoblasts, but also hinted at differentiation to an osteocyte phenotype. These results indicate the successful biofabrication of an in vitro model of the human jawbone, which presents key features of this special bone entity and hence appears promising for application in jawbone-specific research.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

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3D bioprinting of tissue-specific osteoblasts and endothelial cells to model the human jawbone

Amler

Thomas

Tüzüner

et al. 2021

Sci Rep

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“…This intersitespecific variation of osteocyte lacunar density has been shown to be associated with a difference in biomechanical strain occurrence and bone turnover-rate (Zimmermann et al, 2011;Iezzi et al, 2020). In vitro studies demonstrated a higher proliferation rate of patient-matched primary osteoblasts derived from the alveolar bone when compared to the iliac bone, emphasizing a difference in phenotype (Akintoye et al, 2006;Wein et al, 2015;Wein et al, 2019).…”

Section: Surface Extractionmentioning

confidence: 97%

“…The reason for the higher competence of the graft from the iliac crest in large-volume augmentations has not been elucidated to date. In vitro studies using primary human osteoblasts and bone marrow stromal cells from patient-matched alveolar and iliac bone have demonstrated site-specific phenotypic and functional differences regarding their osteogenic potential, proliferation properties, and gene expression profiles (Akintoye et al, 2006;Akintoye et al, 2008;Wein et al, 2015). Current proteome-based analysis of patient-matched human alveolar and iliac bones emphasizes sitespecific differences, with iliac bone predominantly expressing immune-related proteins and alveolar bone ECM-related structural proteins (Fretwurst et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Comparison of the 3D-Microstructure Between Alveolar and Iliac Bone for Enhanced Bioinspired Bone Graft Substitutes

Rothweiler

Groß

Bortel³

et al. 2022

Front. Bioeng. Biotechnol.

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In oral- and maxillofacial bone augmentation surgery, non-vascularized grafts from the iliac crest demonstrate better clinical performance than alveolar bone grafts. The underlying mechanisms are not fully understood but are essential for the enhancement of bone regeneration scaffolds. Synchrotron Radiation µ-CT at a pixel size of 2.3 μm was used to characterize the gross morphology and the vascular and osteocyte lacuna porosity of patient-matched iliac crest/alveolar bone samples. The results suggest a difference in the spatial distribution of the vascular pore system. Fluid simulations reveal the permeability tensor to be more homogeneous in the iliac crest, indicating a more unidirectional fluid flow in alveolar bone. The average distance between bone mineral and the closest vessel pore boundary was found to be higher in alveolar bone. At the same time, osteocyte lacunae density is higher in alveolar bone, potentially compensating for the longer average distance between the bone mineral and vessel pores. The present study comprehensively quantified and compared the 3D microarchitecture of intraindividual human alveolar and iliac bone. The identified difference in pore network architecture may allow a bone graft from the iliac crest to exhibit higher regeneration potential due to an increased capacity to connect with the surrounding pore network of the residual bone. The results may contribute to understanding the difference in clinical performance when used as bone grafts and are essential for optimization of future scaffold materials.

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Oral hard tissue defect models for evaluating the regenerative efficacy of implant materials

Sun

Heng

Zhang

2023

MedComm – Biomaterials and Applications

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Oral hard tissue defects are common concomitant symptoms of oral diseases, which have poor prognosis and often exert detrimental effects on the physical and mental health of patients. Implant materials can accelerate the regeneration of oral hard tissue defects (such as periodontal defects, alveolar bone defects, maxilla bone defects, mandible bone defects, alveolar ridge expansion, and site preservation), but their regenerative efficacy and biocompatibility need to be preclinically validated in vivo with animal‐based oral hard tissue defect models. The choice of oral hard tissue defect model depends on the regenerative effect and intended application of the tested implant material. At the same time, factors that need to be considered include techniques for constructing the particular defect model, the scaffold/graft material used, the availability of animal model evaluation techniques and instrumentation, as well as costs and time constraints. In this article, we summarize the common oral hard tissue defect models in various animal species (such as periodontal model, jaw defect model, and implantation defect model) that can be used to evaluate the regenerative efficacy and biocompatibility of implant materials.

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Pilot investigation of the molecular discrimination of human osteoblasts from different bone entities

Cited by 11 publications

References 37 publications

3D bioprinting of tissue-specific osteoblasts and endothelial cells to model the human jawbone

3D bioprinting of tissue-specific osteoblasts and endothelial cells to model the human jawbone

Comparison of the 3D-Microstructure Between Alveolar and Iliac Bone for Enhanced Bioinspired Bone Graft Substitutes

Oral hard tissue defect models for evaluating the regenerative efficacy of implant materials

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