Pilose antler peptide promotes osteoblast proliferation, differentiation and mineralization via the insulin signaling pathway

ChangJun, Yun; Qian, Wenjie; Wu, Junyi; Yuan, Chenxi; Jiang, Songzhou; Lv, Jinpeng

doi:10.3892/etm.2019.8286

Cited by 10 publications

(13 citation statements)

References 24 publications

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“…Since Ras can activate both the Raf/MEK/ERK and the PI3K/Akt pathways [31], they develop close interactions during regeneration. In the context of Xenopus froglet limb and deer antler regeneration, the MAPK/ERK and PI3K/Akt pathways are excited simultaneously and collaboratively to drive cell cycle re-entry and dedifferentiation as well as suppress apoptosis [35,43], while to achieve peripheral nerve regeneration, they function in parallel in separate aspects [82]. In more complex contexts, such as CNS regeneration, ERK activity needs to be transiently suppressed to allow other signaling pathways to set in to induce neuron/glial progenitor cell differentiation.…”

Section: Discussionmentioning

confidence: 99%

“…Proteomics analysis of regenerating red deer antler indicates MAPK/ERK signaling is activated in both pedicle periosteum and antlerogenic periosteum cells, which are the major cell sources to form the antler bud [44]. By investigating a component protein purified from deer antler tissue named pilose antler peptide (PAP), Yun et al [43] elucidates how the administration of PAP strongly activates insulin signaling and hierarchically stimulates ERK and PI3K/Akt signaling, to promote osteoblasts proliferation, differentiation, and mineralization.…”

Section: Mapk/erk Pathway In Appendage Regenerationmentioning

confidence: 99%

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MAPK/ERK Pathway as a Central Regulator in Vertebrate Organ Regeneration

Wen

Jiao

Tan

2022

IJMS

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Damage to organs by trauma, infection, diseases, congenital defects, aging, and other injuries causes organ malfunction and is life-threatening under serious conditions. Some of the lower order vertebrates such as zebrafish, salamanders, and chicks possess superior organ regenerative capacity over mammals. The extracellular signal-regulated kinases 1 and 2 (ERK1/2), as key members of the mitogen-activated protein kinase (MAPK) family, are serine/threonine protein kinases that are phylogenetically conserved among vertebrate taxa. MAPK/ERK signaling is an irreplaceable player participating in diverse biological activities through phosphorylating a broad variety of substrates in the cytoplasm as well as inside the nucleus. Current evidence supports a central role of the MAPK/ERK pathway during organ regeneration processes. MAPK/ERK signaling is rapidly excited in response to injury stimuli and coordinates essential pro-regenerative cellular events including cell survival, cell fate turnover, migration, proliferation, growth, and transcriptional and translational activities. In this literature review, we recapitulated the multifaceted MAPK/ERK signaling regulations, its dynamic spatio-temporal activities, and the profound roles during multiple organ regeneration, including appendages, heart, liver, eye, and peripheral/central nervous system, illuminating the possibility of MAPK/ERK signaling as a critical mechanism underlying the vastly differential regenerative capacities among vertebrate species, as well as its potential applications in tissue engineering and regenerative medicine.

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Section: Discussionmentioning

confidence: 99%

Section: Mapk/erk Pathway In Appendage Regenerationmentioning

confidence: 99%

MAPK/ERK Pathway as a Central Regulator in Vertebrate Organ Regeneration

Wen

Jiao

Tan

2022

IJMS

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“…Yun et al reported that deer antler peptide promoted osteoblast proliferation, differentiation, and mineralization in vitro via the insulin signaling pathway. The effect of deer antler peptide on insulin signaling in osteoblasts may be mediated via the ERK pathway and partially by the PI3K/Akt pathway [ 30 ]. Chen et al reported that velvet antler dietary supplementation has beneficial effects on femur development in growing rats [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

Use of Network Pharmacology and Molecular Docking Technology to Analyze the Mechanism of Action of Velvet Antler in the Treatment of Postmenopausal Osteoporosis

Guo

Wang

Luo

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Deer velvet antlers are the young horns of male deer that are not ossified and densely overgrown. Velvet antler and its preparations have been widely used in the treatment of postmenopausal osteoporosis (PMOP) in recent years, although its mechanism of action in the human body remains unclear. To screen the effective ingredients and targets of velvet antler in the treatment of PMOP using network pharmacology and to explore the potential mechanisms of velvet antler action in such treatments, we screened the active ingredients and targets of velvet antler in the BATMAN-TCM database. We also screened the relevant targets of PMOP in the GeneCards and OMIM databases and then compared the targets at the intersection of both velvet antler and PMOP. We used Cytoscape 3.7.2 software to construct a network diagram of “disease-drug-components-targets” and a protein-protein interaction (PPI) network through the STRING database and screened out the core targets; the R language was then used to analyze the shared targets between antler and PMOP for GO-enrichment analysis and KEGG pathway-annotation analysis. Furthermore, we used the professional software Maestro 11.1 to verify the predictive analysis based on network pharmacology. Hematoxylin-eosin (H&E) staining and micro-CT were used to observe the changes in trabecular bone tissue, further confirming the results of network pharmacological analysis. The potentially effective components of velvet antler principally include 17β-E2, adenosine triphosphate, and oestrone. These components act on key target genes such as AKT1, IL6, MAPK3, TP53, EGFR, SRC, and TNF and regulate the PI3K/Akt-signaling and MAPK-signaling pathways. These molecules participate in a series of processes such as cellular differentiation, apoptosis, metabolism, and inflammation and can ultimately be used to treat PMOP; they reflect the overall regulation, network regulation, and protein interactions.

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“…Cell viability was examined by MTT assay ( Yun et al, 2020 ). Briefly, the cells were seeded in 96-well plates and treated with J147 (1–8 μM) for 48 h. Then, the cells were washed with PBS and replaced with MTT solution (20 μL).…”

Section: Methodsmentioning

confidence: 99%

The Inhibitory Effect of Curcumin Derivative J147 on Melanogenesis and Melanosome Transport by Facilitating ERK-Mediated MITF Degradation

Yang

Jia

et al. 2021

Front. Pharmacol.

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The therapeutic use of curcumin and chemically modified curcumin (CMC) for suppressing melanogenesis and tyrosinase activity have been recognized. J147 is a modified version of curcumin with superior bioavailability and stability. However, there is no report about the effects of J147 on pigmentation in vitro and in vivo. In our studies, we investigated the hypopigmentary effects of J147 treatment on melanocytes and explored the underlying mechanism. The present studies suggested that J147 suppressed both basal and α-MSH-induced melanogenesis, as well as decreased melanocyte dendricity extension and melanosome transport. J147 played these roles mainly by activating the extracellular signal-regulated protein kinase (ERK) pathway. Once activated, it resulted in MITF degradation and further down-regulated the expression of tyrosinase, TRP-1, TRP-2, Myosin Va, Rab27a and Cdc42, ultimately inhibited melanin synthesis and melanosome transport. Furthermore, the hypopigmentary effects of J147 were demonstrated in vivo in a zebrafish model and UVB-induced hyperpigmentation model in brown guinea pigs. Our findings also suggested that J147 exhibited no cytotoxicity in vitro and in vivo. Taken together, these data confirmed that J147 may prove quite useful as a safer natural skin-whitening agent.

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Pilose antler peptide promotes osteoblast proliferation, differentiation and mineralization via the insulin signaling pathway

Cited by 10 publications

References 24 publications

MAPK/ERK Pathway as a Central Regulator in Vertebrate Organ Regeneration

MAPK/ERK Pathway as a Central Regulator in Vertebrate Organ Regeneration

Use of Network Pharmacology and Molecular Docking Technology to Analyze the Mechanism of Action of Velvet Antler in the Treatment of Postmenopausal Osteoporosis

The Inhibitory Effect of Curcumin Derivative J147 on Melanogenesis and Melanosome Transport by Facilitating ERK-Mediated MITF Degradation

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