Pili of oral Streptococcus sanguinis bind to fibronectin and contribute to cell adhesion

Okahashi, Nobuo; Nakata, Masao; Sakurai, Atsuo; Terao, Yutaka; Hoshino, T.; Yamaguchi, M.; Isoda, Ryutaro; Sumitomo, Tomoko; Nakano, Kazuhiko; Kawabata, Shigetada; Ooshima, Takashi

doi:10.1016/j.bbrc.2009.12.029

Cited by 41 publications

(42 citation statements)

References 31 publications

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“…We speculated that the increase in surface pili would lead to a biofilm phenotype. As cell appendices, pili are important for the adherence of several bacterial species to host tissue and other surfaces (41,47,67) and also have been discussed as important in biofilm formation (32,40). In agreement with our hypothesis, we detected a significant increase of biomass attached to the polystyrene surface by the covRS mutant in the standard crystal violet biofilm assay.…”

Section: Vol 193 2011supporting

confidence: 90%

Counteractive Balancing of Transcriptome Expression Involving CodY and CovRS in Streptococcus pyogenes

et al. 2011

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Section: Vol 193 2011supporting

confidence: 90%

Counteractive Balancing of Transcriptome Expression Involving CodY and CovRS in Streptococcus pyogenes

et al. 2011

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“…In addition, the capacity to adhere to ECM proteins has been suggested to be an important factor in the colonization of the heart valves by oral bacteria (48,53,56). Similar to the case for S. gallolyticus (62), our data revealed that the capacity to invade does not seem to be associated with the ability to form biofilms and that Cnm plays a strain-specific role in biofilm formation.…”

Section: Discussionmentioning

confidence: 99%

The Collagen-Binding Protein Cnm Is Required for Streptococcus mutans Adherence to and Intracellular Invasion of Human Coronary Artery Endothelial Cells

et al. 2011

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Streptococcus mutans is considered the primary etiologic agent of dental caries, a global health problem that affects 60 to 90% of the population, and a leading causative agent of infective endocarditis. It can be divided into four different serotypes (c, e, f, and k), with serotype c strains being the most common in the oral cavity. In this study, we demonstrate that in addition to OMZ175 and B14, three other strains (NCTC11060, LM7, and OM50E) of the less prevalent serotypes e and f are able to invade primary human coronary artery endothelial cells (HCAEC). Invasive strains were also significantly more virulent than noninvasive strains in the Galleria mellonella (greater wax worm) model of systemic disease. Interestingly, the invasive strains carried an additional gene, cnm, which was previously shown to bind to collagen and laminin in vitro. Inactivation of cnm rendered the organisms unable to invade HCAEC and attenuated their virulence in G. mellonella. Notably, the cnm knockout strains did not adhere to HCAEC as efficiently as the parental strains did, indicating that the loss of the invasion phenotype observed for the mutants was linked to an adhesion defect. Comparisons of the invasive strains and their respective cnm mutants did not support a correlation between biofilm formation and invasion. Thus, Cnm is required for S. mutans invasion of endothelial cells and possibly represents an important virulence factor of S. mutans that may contribute to cardiovascular infections and pathologies.

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“…Attachment of S. sanguinis to substrates is mostly mediated by adhesins present on the bacterial cell wall surface, 12 with some studies even describing the existence of bacterial appendages such as pili as contributing factors in adhesion. 13 In order to understand the initial bacteria-substrate interaction, the process of bacterial adhesion has been characterized according to biophysical models into a "docking phase" and a "locking phase".…”

Section: Introductionmentioning

confidence: 99%

“…17 Bacterial appendages such as pili and capsule are also thought to be involved in this irreversible locking phase stage, where surface de-adhesion can only be obtained by mechanical or chemical removal. 13,18 Although several methodologies have previously been employed to study bacterial adhesion and colonization to biomedical substrates, the use of atomic force microscopy (AFM) opens new possibilities to characterize the nanoadhesion of bacteria to surfaces with nanonewton and piconewton sensitivity. 19 In this context, functionalized live-bacterium probes allow the possibility to explore bacterium-surface nanoadhesive interactions by employing techniques such as single-cell force spectroscopy (SCFS).…”

Section: Introductionmentioning

confidence: 99%

Probing the nanoadhesion of Streptococcus sanguinis to titanium implant surfaces by atomic force microscopy

Aguayo¹,

Donos²,

Spratt³

et al. 2016

IJN

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As titanium (Ti) continues to be utilized in great extent for the fabrication of artificial implants, it is important to understand the crucial bacterium–Ti interaction occurring during the initial phases of biofilm formation. By employing a single-cell force spectroscopy technique, the nanoadhesive interactions between the early-colonizing Streptococcus sanguinis and a clinically analogous smooth Ti substrate were explored. Mean adhesion forces between S. sanguinis and Ti were found to be 0.32±0.00, 1.07±0.06, and 4.85±0.56 nN for 0, 1, and 60 seconds contact times, respectively; while adhesion work values were reported at 19.28±2.38, 104.60±7.02, and 1,317.26±197.69 aJ for 0, 1, and 60 seconds, respectively. At 60 seconds surface delays, minor-rupture events were modeled with the worm-like chain model yielding an average contour length of 668±12 nm. The mean force for S. sanguinis minor-detachment events was 1.84±0.64 nN, and Poisson analysis decoupled this value into a short-range force component of −1.60±0.34 nN and a long-range force component of −0.55±0.47 nN. Furthermore, a solution of 2 mg/mL chlorhexidine was found to increase adhesion between the bacterial probe and substrate. Overall, single-cell force spectroscopy of living S. sanguinis cells proved to be a reliable way to characterize early-bacterial adhesion onto machined Ti implant surfaces at the nanoscale.

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Pili of oral Streptococcus sanguinis bind to fibronectin and contribute to cell adhesion

Cited by 41 publications

References 31 publications

Counteractive Balancing of Transcriptome Expression Involving CodY and CovRS in Streptococcus pyogenes

Counteractive Balancing of Transcriptome Expression Involving CodY and CovRS in Streptococcus pyogenes

The Collagen-Binding Protein Cnm Is Required for Streptococcus mutans Adherence to and Intracellular Invasion of Human Coronary Artery Endothelial Cells

Probing the nanoadhesion of Streptococcus sanguinis to titanium implant surfaces by atomic force microscopy

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