PIK3CA and TFRC Located in 3q Are New Prognostic Factors in Esophageal Squamous Cell Carcinoma

Wada, Shinsuke; Noguchi, Tsuyoshi; Takeno, Shinsuke; Kawahara, Katsunobu

doi:10.1245/aso.2006.08.006

Cited by 36 publications

(39 citation statements)

References 31 publications

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“…Transferrin receptor has been described as an independent prognostic factor based on mRNA expression analysis in ESCC. 42 In the current study, TFRC and TF were 4.3-and 14.9-fold upregulated in the ESCC secretome.…”

supporting

confidence: 45%

SILAC-based quantitative proteomic approach to identify potential biomarkers from the esophageal squamous cell carcinoma secretome

Kashyap

Harsha

Renuse

et al. 2010

Cancer Biology & Therapy

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supporting

confidence: 45%

SILAC-based quantitative proteomic approach to identify potential biomarkers from the esophageal squamous cell carcinoma secretome

Kashyap

Harsha

Renuse

et al. 2010

Cancer Biology & Therapy

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“…In PIK3CA mRNA, it has been reported that PIK3CA mRNA expression correlated with the pN status in ESCC (43). However, in this study, expression levels of mRNA were investigated only with qualitative analysis using formalin-fixed samples (43).…”

mentioning

confidence: 92%

“…In our study, we failed to detect the correlation between the PIK3CA mRNA status and pN status. On the other hand, a recent study highlighted a significant correlation between the PIK3CA mRNA and pN status (43). The lack of association between PIK3CA mRNA and protein could be due to transcriptional or posttranscriptional mechanisms which finely control the expression level of its protein.…”

mentioning

confidence: 97%

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Overexpression of PIK3CA is associated with lymph node metastasis in esophageal squamous cell carcinoma

Akagi

Miyashita²,

Makino³

et al. 2009

Int J Oncol

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Abstract. The genomic region containing PIK3CA was found to be amplified in esophageal squamous cell carcinoma (ESCC) tissue. PIK3CA at 3q26, which encodes the p110· catalytic subunit of phosphatidylinositol (PI) 3-kinase, is a unique intracellular signal transducer characterized by its lipid substrate specificity. In order to characterize PIK3CA in ESCC, we investigated hot-spot mutations in exons 1, 9 and 20, the copy number gain, the expression levels of mRNA and protein. Analysis in exon 9 of the PIK3CA gene revealed mutation in 7.7% (4 of 52) of ESCC samples. No mutation was detected in either exon 1 or exon 20. Copy number amplifications of PIK3CA were found in 12 of the 45 patients (26.7%). PIK3CA mRNAs were examined in 37 ESCC patients as determined by quantitative RT-PCR and the mean mRNA level of PIK3CA in ESCC tissues was 2.61-fold higher compared with that in corresponding non-tumorous esophageal epithelia (P<0.001). Immunohistochemically, positive immunoreaction for PIK3CA was detectable in 33 of 66 (50.0%) ESCC cases, while it was not detectable in the remaining 33 cases. Furthermore, comparing the cases with negative staining with those with positive staining for PIK3CA, the presence of node metastasis was significantly correlated with those with positive staining (P<0.05). This study is the first report providing comprehensive analysis of PIK3CA expression in ESCC. These results indicate that PIK3CA may play a crucial role in the development of ESCC and serve as an indicator for lymph node metastasis.

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“…ECT2, coding for a transforming protein that is related to Rho-specific exchange factors, plays a role in epithelial cell polarity (Liu et al, 2006a). Finally, PIK3CA encodes the p110a catalytic subunit of the class IA phosphatidylinositol 3-kinases (PI3Ks), which is involved in tumor cell proliferation as well as in invasion and metastasis (Samuels and Ericson, 2006), the PIK3CA mRNA levels correlate with worse prognosis in patients with ESCC (Wada et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Amplification of PRKCI, located in 3q26, is associated with lymph node metastasis in esophageal squamous cell carcinoma

Yang

Chu

Luo

et al. 2007

Genes Chromosomes & Cancer

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DNA amplification is one of the mechanisms to activate genes that are implicated in neoplastic transformation and gain of chromosome band 3q26 is a common event in squamous cell carcinomas. The aim of the present work was to identify the specific target gene from four candidates (MDS1, PRKCI, ECT2, and PIK3CA) located on 3q26 amplification in esophageal squamous cell carcinomas (ESCCs). To assess the prevalence of copy number gains of putative genes, fluorescence in situ hybridization (FISH) was applied on 108 ESCCs and 9 ESCC cell lines. Our data showed that MDS1 and PRKCI were more frequently gained. Positive correlation was found only for PRKCI between amplification and tumor size (P = 0.043), lymph node metastasis (P = 0.015) and clinical stage (P = 0.002). PRKCI gene amplification was highly correlated with protein overexpression (P = 0.009), suggesting that gene amplification is one important mechanism involved in PRKCI overexpression. To investigate further the role of PRKCI alteration in esophageal tumors, a tissue microarray containing samples from 180 ESCCs was used for immunohistochemistry analysis. Statistical analysis revealed that PRKCI overexpression was correlated with lymph node metastasis (P = 0.002) and higher stage (P = 0.004). Performing multivariate logistic regression analysis, a significant association between PRKCI overexpression and presence of lymph node metastasis was found, which was independent of T-stage of the primary tumors (P = 0.004). Our results indicate that PRKCI is an attractive target in the 3q26 amplicon and that it may serve as a molecular marker for metastasis and occult advanced tumor stages in ESCC.

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PIK3CA and TFRC Located in 3q Are New Prognostic Factors in Esophageal Squamous Cell Carcinoma

Abstract: PIK3CA and TFRC mRNA represent prognostic factors in patients with ESCC. TFRC mRNA offers an independent prognostic factor, and expression may have clinically important implications.

Cited by 36 publications

References 31 publications

SILAC-based quantitative proteomic approach to identify potential biomarkers from the esophageal squamous cell carcinoma secretome

SILAC-based quantitative proteomic approach to identify potential biomarkers from the esophageal squamous cell carcinoma secretome

Overexpression of PIK3CA is associated with lymph node metastasis in esophageal squamous cell carcinoma

Amplification of PRKCI, located in 3q26, is associated with lymph node metastasis in esophageal squamous cell carcinoma

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