Pigment epithelium-derived factor (PEDF)-induced apoptosis and inhibition of vascular endothelial growth factor (VEGF) expression in MG63 human osteosarcoma cells

Takenaka, Katsuto; Yamagishi, Sho‐ichi; Jinnouchi, Yuko; Nakamura, Kazuo; Matsui, Takanori; Imaizumi, Tsutomu

doi:10.1016/j.lfs.2005.05.048

Cited by 95 publications

(87 citation statements)

References 28 publications

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“…29,30 PEDF has been shown to be the most potent inhibitor of angiogenesis in the mammalian eye, suggesting that decreased levels of PEDF may be a factor in the development of angiogenic eye diseases such as PDR. 31,32 PEDF levels have also been found to be positively linked to retinal oxygen concentrations which have been found to be inversely related to VEGF concentrations in a balance controlling angiogenesis. 33 Cheung and colleagues in 2006 found that ER is an important upstream regulator of PEDF in human ovarian cancer and ovarian surface epithelial cells.…”

mentioning

confidence: 99%

Effects of Tamoxifen Versus Raloxifene on Retinal Capillary Endothelial Cell Proliferation

Grigsby

Parvathaneni

Almanza

et al. 2011

Journal of Ocular Pharmacology and Therapeutics

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Purpose: Endothelial cell proliferation in angiogenesis is active in conditions such as cancers and diabetic retinopathy. Tamoxifen (T) and raloxifene (R) have been compared in numerous studies as a prophylaxis for breast cancer, and T is used to treat breast cancer. T, unlike R, has been linked to an increase in uterine cancers, thrombo-embolic events, and cataract. The purpose of our study was to evaluate the efficacies of T and R in reducing estrogen-induced retinal capillary endothelial cell proliferation. Methods: Rhesus monkey retinal capillary endothelial cells (ATCC RF/6A) were used to assay cell proliferation when treated with 0.0, 0.1, 1.0, and 10.0 nM 17 b estradiol (E2) for 24 and 48 h. Viable cells were counted using a Neubauer hemocytometer with a trypan blue exclusion method to determine the number of viable cells. Cell counts were also performed using 1.0 nM E2 with 0.01, 0.1, 1.0, and 10.0 nM concentrations of either T or R. Cell medium, collected at 24 h, was evaluated for vascular endothelial growth factor and pigment epithelium-derived factor. Results: Viable cells were significantly greater in cultures treated with 1.0 or 10.0 nM E2, compared to cells treated with 0.0 or 0.1 nM E2 both at 24 and 48 h. Viable cell counts were reduced significantly in cultures treated with 0.1, 1.0, or 10.0 nM T or R in addition to the 1.0 nM E2. Cell counts were not significantly different when comparing equal concentrations of T and R, that is, 1.0 nM E2 + 1 nM T or R. Vascular endothelial growth factor and pigment epithelium-derived factor protein/10,000 cells was reduced by 1.0 nM E2, but returned to higher levels with the introduction of T and R to growth media. Conclusions: T and R showed similar potency in inhibiting estrogen-induced retinal capillary endothelial cell proliferation. Considering drug safety profiles, our results, when extended to animals and humans, suggest that R is preferable to T in treating angiogenic retinal diseases. Further studies on the signaling mechanism of estrogen-induced endothelial cell proliferation may lead to new treatment strategies in the treatment of ocular angiogenic diseases.

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confidence: 99%

Effects of Tamoxifen Versus Raloxifene on Retinal Capillary Endothelial Cell Proliferation

Grigsby

Parvathaneni

Almanza

et al. 2011

Journal of Ocular Pharmacology and Therapeutics

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“…It has been shown to be the most important inhibitor of angiogenesis in mammalian eyes, strongly suggesting that decreased levels of it play an important role in angiogenic eye diseases such as proliferative diabetic retinopathy (Dawson et al 1999;Takenaka et al 2005). Gao et al, using Brown Norway rats, found PEDF levels to be inversely related to VEGF levels.…”

Section: Angiogenesismentioning

confidence: 99%

The Role of Sex Hormones in Diabetic Retinopathy

Grigsby¹,

Allen²,

Culbert³

et al. 2012

Diabetic Retinopathy

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“…Although the receptor for PEDF on ECs remains largely unknown, this factor has been shown to inhibit EC migration (Duh et al, 2002) and to stimulate apoptosis by activating the FAS-FasL death pathway (Volpert et al, 2002). Moreover, PEDF blocks the production of angiogenic growth factors from tumour cells and stimulates g-secretase-dependent cleavage of VEGFR1, acting as an inhibitor of VEGF signalling (Volpert et al, 2002;Takenaka et al, 2005;Cai et al, 2006). The inhibitory effects of Rb1 observed by the authors with respect to Matrigel-driven network formation might be related to the induction of apoptosis or the blockade of cell motility that affects tube-like network formation on Matrigel.…”

Section: Ginsenosides Altering Angiogenic Responsesmentioning

confidence: 99%

A ginseng‐derived oestrogen receptor β (ERβ) agonist, Rb1 ginsenoside, attenuates capillary morphogenesis

Papapetropoulos

2007

British J Pharmacology

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A ginseng-derived oestrogen receptor b (ERb) agonist, Rb1 ginsenoside, attenuates capillary morphogenesis A PapapetropoulosLaboratory of Molecular Pharmacology, Department of Pharmacy, University of Patras, Patras, GreeceGinseng extracts contain a variety of active ingredients and have been shown to promote or inhibit angiogenesis, depending on the presence of different ginsenosides that exert opposing effects on blood vessel growth. Leung et al. in this issue of the British Journal of Pharmacology report that Rb1, a ginsenoside that constitutes only 0.37-0.5% of ginseng extracts (depending on manufacturing and processing methods), blocks tube-like network formation by endothelial cells in vitro. At the molecular level, Rb1 binds to the oestrogen receptors and stimulates the transcription of pigment epithelium-derived factor that, in turn, inhibits matrix-driven capillary morphogenesis.

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Pigment epithelium-derived factor (PEDF)-induced apoptosis and inhibition of vascular endothelial growth factor (VEGF) expression in MG63 human osteosarcoma cells

Cited by 95 publications

References 28 publications

Effects of Tamoxifen Versus Raloxifene on Retinal Capillary Endothelial Cell Proliferation

Effects of Tamoxifen Versus Raloxifene on Retinal Capillary Endothelial Cell Proliferation

The Role of Sex Hormones in Diabetic Retinopathy

A ginseng‐derived oestrogen receptor β (ERβ) agonist, Rb1 ginsenoside, attenuates capillary morphogenesis

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