Piglets Born after Vitrification of Embryos Using the Open Pulled Straw Method

Berthelot, F.; Martinat-Botté, F.; Locatelli, A.; Perreau, C.; Terqui, M.

doi:10.1006/cryo.2000.2273

Cited by 102 publications

(72 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…-with the OPS method, we obtained hatched blastocysts in vitro after morula vitrification [2]; -after cytochalasin treatment and vitrification of 17 morulae, Dobrinsky et al [6] obtained one morula-to-blastocyst development in vitro, but no hatching.…”

Section: Introductionmentioning

confidence: 94%

See 1 more Smart Citation

Birth of piglets after OPS vitrification and transfer of compacted morula stage embryos with intact zona pellucida

et al. 2001

Self Cite

View full text Add to dashboard Cite

-In swine, five to six days post-insemination, morulae and blastocysts are collected together after uterine flushing. The purpose of this study was to vitrify zona pellucida-intact morulae with Open Pulled Straw (OPS) technology and obtain piglets after transfer. Morulae (200) were vitrified after a two-step equilibration in ethylene glycol, dimethyl sulfoxide and sucrose in Hepesbuffered TCM199 + 20% NBCS medium (TCM). 2-6 morulae were loaded into OPS and plunged into liquid nitrogen. At embryo warming, a three-step dilution with decreasing concentrations of sucrose was applied. In each of 10 recipients, 20 morulae were transferred surgically. Day 25, gestation rate and the farrowing rate were 80% and 70%, respectively. The pregnant recipients farrowed from 1 to 8 piglets and the survival of total transferred embryos was 13%. Although survival rates are still compromised, OPS technology is therefore appropriate to cryopreserve porcine morulae with intact zona pellucida. pig / embryo / morula / OPS vitrification / cryopreservationRésumé -Naissance de porcelets après transfert de morulae dans la zone pellucide intacte et vitrification par la méthode OPS. Chez la truie, 5 à 6 jours après l'insémination, il est récolté des embryons aux stades morula et blastocyste associés. Le but de ce travail est de transférer des morulae vitrifiées et d'obtenir des porcelets. Des morulae (200) sont vitrifiées par la méthode OPS après 2 étapes d'équilibration dans un mélange égal d'éthylène glycol, de DMSO et de sucrose dilués dans du TCM199 Hépès + 20 % de sérum de veau nouveau-né (TCM). Pour le réchauffement, les paillettes sont sorties de l'azote et plongées immédiatement dans du TCM + sucrose, l'élimination des cryoprotecteurs se faisant en 3 étapes. Pour évaluer la viabilité des morulae vitrifiées/réchauffées, 10 receveuses reçoivent chacune 20 morulae après transfert chirurgical. Le pourcentage de mises-bas Reprod. Nutr. Dev. 41 (2001) 267-272 267

show abstract

Section: Introductionmentioning

confidence: 94%

“…Previously, the birth of piglets has been reported after vitrification and warming at the unhatched blastocyst stage [1,2,3,11]. To our knowledge, there is no report of a successful transfer of vitrified/warmed morulae with intact zona pellucida.…”

Section: Introductionmentioning

confidence: 99%

Birth of piglets after OPS vitrification and transfer of compacted morula stage embryos with intact zona pellucida

et al. 2001

Self Cite

View full text Add to dashboard Cite

show abstract

“…A number of new techniques have recently been developed to increase the cooling and warming rates of vitrification, including the Solid Surface Vitrification (SSV; Dinnyés et al, 2000;Bagis et al, 2004;Somfai et al, 2006), Minimum Volume Cooling (MVC; Kuwayama and Kato, 2000) and Open Pulled Straw (OPS; Vajta et al, 1997;Vajta et al, 1998;Berthelot et al, 2000) methods. The effectiveness of these techniques is different.…”

Section: Discussionmentioning

confidence: 99%

Vitrification of in vitro matured oocytes of Mangalica and Large White pigs

Varga

Gajdócsi

Makkosné

et al. 2008

Acta Veterinaria Hungarica

View full text Add to dashboard Cite

The breeding of Mangalica, a native pig breed in Hungary, had been started in 1833, but this pig breed almost became extinct in Hungary in the past decades. In 1991, the number of sows was only 200. Although in these days the existing Mangalica population consists of more than 6000 animals representing different colour variations, the preservation of this traditional pig breed is still very important. Vitrification is a potential tool for the preservation of gametes and embryos of these animals. The aim of this study was to investigate the effects of vitrification on the developmental competence of Mangalica (M) and Large White (LW) oocytes following fertilisation. The oocytes were vitrified by the Open Pulled Straw (OPS) method using different concentrations of ethylene glycol and dimethyl sulphoxide as cryoprotectants. After rehydration the oocytes underwent in vitro fertilisation; the resultant zygotes were then cultured in vitro for four days to assess embryonic development. In the first experiment, in vitro maturation of M and LW oocytes was compared. No significant difference was observed in the nuclear maturation rate of LW and M oocytes. In the second experiment, the sensitivity of oocytes to vitrification was examined by evaluating oocyte morphology after thawing. A higher percentage of LW oocytes showed normal morphology compared to M oocytes, indicating that Mangalica oocytes are more sensitive to cryoprotectants than Large White oocytes. After warming and in vitro fertilisation, more than 50% of the oocytes started embryonic development and by the end of the incubation period morula stage embryos had developed in both groups. The results show that the OPS vitrification technique is well suited to preserve Mangalica oocytes and from these oocytes morula embryos can be produced.

show abstract

“…Transfer of 147 vitrified expanded blastocysts into 10 recipients, resulted in birth of 29 live piglets, with pregnancy rate of 50% (Figure 2; Gajda et al, 2004). Recent application of the OPS method to pig blastocysts has allowed excellent in vitro survival (Berthelot et al, 2000(Berthelot et al, , 2001Gajda and Smorąg, 2001), but pregnancy rates after transfer are quite variable, ranging from zero to 60% (Gajda et al, 2006). …”

Section: Speciesmentioning

confidence: 99%

“…The OPS method offers several advantages: extremely fast cooling and warming rates of more than 20 000 o C/min, brief contact with concentrated cryoprotective additives (less than 30 sec over a range of 130 o C), and the possibility of avoiding injury due to chilling and of decreasing toxic and osmotic damage. With the introduction of the open pulled straw in 1997, the successful vitrification of embryos and oocytes from several different species have been reported: early stage bovine embryos produced in vitro (Vajta et al, 1997a), morula and blastocyst of pig (Berthelot et al, 2000;Gajda et al, 2006), horse (Oberstein et al, 2001) and cow (Hyttel et al, 2000), and porcine oocytes (Vajta et al, 1997b). Using this technique calves have been born following the transfer of embryos vitrified at both the oocyte and blastocyst stages Le Gal et al, 2000).…”

Section: Cryotop (Also Called Minimum-volume Cooling)mentioning

confidence: 99%

Oocyte and embryo cryopreservation – state of art and recent developments in domestic animals

Gajda¹,

Smorąg²

2009

J. Anim. Feed Sci.

View full text Add to dashboard Cite

During the past few decades, significant progress in cryopreservation of mammalian oocytes and embryos has been observed. Transfer of cryopreserved embryos or oocytes resulted in live offspring in at least 25 species. So far, two major methods have been used for oocyte and embryo cryopreservation: conventional slow-rate freezing and vitrification. This review summarizes the progress in cryopreservation of mammalian oocytes and embryos that has been achieved by modifying oocyte/embryo susceptibility to cryopreservation or vitrification technology through such techniques as solid-surface vitrification, cryoloop, microdrop, cryotop, electron microscopy grids, nylon mesh, open pulled straw method or removal of lipids, addition of antifreeze protein or cytoskeletonstabilizing agents, cholesterol or liposomes, centrifugation prior to cryopreservation or application of high hydrostatic pressure. In conclusion, the vitrification method opened new perspectives in cryopreservation of embryos and oocytes, both for in vitro fertilized and somatic nuclear transfer. Authors believe that new cryopreservation procedures which modify the susceptibility of oocytes and embryos to cryopreservation will gain importance as a major tool in mammalian gamete and embryo cryopreservation.

show abstract

Piglets Born after Vitrification of Embryos Using the Open Pulled Straw Method

Cited by 102 publications

References 18 publications

Birth of piglets after OPS vitrification and transfer of compacted morula stage embryos with intact zona pellucida

Birth of piglets after OPS vitrification and transfer of compacted morula stage embryos with intact zona pellucida

Vitrification of in vitro matured oocytes of Mangalica and Large White pigs

Oocyte and embryo cryopreservation – state of art and recent developments in domestic animals

Contact Info

Product

Resources

About