Piglet Model for Studying Esophageal Regrowth after Resection and Interposition of a Silicone Stented Small Intestinal Submucosa Tube

Jönsson, Linus; Gatzinsky, Vladimir; Jennische, Eva; Johansson, Carina B.; Nannmark, Ulf; Friberg, Lars Göran

doi:10.1159/000324401

Cited by 14 publications

(16 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Intense angiogenesis was seen in three of six pigs where accumulation of immature‐looking cells arranged in a muscle‐like pattern was present in two of them, 4 and 17 weeks after the operation, and angiogenesis without accumulation of immature‐looking cells 10 days after surgery was present in one. The other three piglets showed only scar tissue formation with enhanced inflammation including perivascular islets of lymphocytes in the bridging area .…”

mentioning

confidence: 93%

See 1 more Smart Citation

Early Regenerative Response in the Intrathoracic Porcine Esophagus—The Impact of the Inflammation

et al. 2013

View full text Add to dashboard Cite

In order to improve the treatment of children born with long-gap esophageal atresia, a porcine model was developed for studying esophageal regrowth using a bridging graft composed of a silicone stented Biodesign mesh. The aim of the study was to investigate how leakage and contact between the native muscle and Biodesign mesh affected the early healing response. Resection of 3 cm of intrathoracic esophagus was performed in 10 newly weaned piglets. They were fed through a gastrostomy 8-10 days prior to sacrifice. In order to achieve nonleaking anastomoses, the silicone stent and suturing technique had to be adjusted between the first four and second six piglets. The technical adjustment decreased leakage. A nonleaking anastomosis could not be achieved when the native muscle layers were sewn less central on the bridging graft compared with the mucosa. If there was leakage, the inflammatory response increased, with islets of perivascular T-lymphocytes and infiltration of macrophages in the native muscle layers. In the bridging area, new vessels were seen in the submucosa in 9 of 10 piglets between 4 and 10 days after surgery. Smooth muscle cells also appeared to move from the cut muscle edges of both the muscularis mucosa and the lamina muscularis and were seen as a layer of several cells under newly formed mucosa. Double staining of the basal membrane of the ingrowing vessels and the pericytes showed that the basal membrane was thinner over some of the pericytes, but there was no accumulation of immature-looking cells in the submucosa of the bridging area. In this porcine model, where esophageal regrowth was studied by using a bridging graft composed of a silicone stented Biodesign mesh, we can conclude that leakage increased the inflammatory response in early healing. Ingrowth of new vessels was seen in the bridging area and movement of smooth muscle cells was found under newly formed mucosa.

show abstract

mentioning

confidence: 93%

“…In order to improve the treatment for children with long‐gap EA, we have developed a porcine guided tissue regeneration model for studying esophageal regrowth after removal of 3 cm of the intrathoracic esophagus .…”

mentioning

confidence: 99%

Early Regenerative Response in the Intrathoracic Porcine Esophagus—The Impact of the Inflammation

et al. 2013

View full text Add to dashboard Cite

show abstract

“…In previous studies, signs of a regenerative healing response in the esophagus of growing piglets were seen when using a silicone-stented Biodesign mesh to bridge a 3-cm long resection of the thoracic esophagus (5). Nine days after surgery, the bridging area was covered with white tissue, made of different bone marrow-derived cells, newly formed vessels, connective tissue, and a thin layer of smooth muscle cells.…”

Section: Discussionmentioning

confidence: 94%

“…Six newly weaned piglets were operated according to our previously described method (5). Briefly, a 3-cm long resection of the intrathoracic esophagus was performed through a right-sided thoracotomy.…”

Section: Surgical Proceduresmentioning

confidence: 99%

“…In order to improve the treatment for children with long-gap EA, we have developed a porcine guided tissue regeneration model for studying esophageal regrowth after removal of 3 cm of the intrathoracic esophagus (5). Previous animal studies of circumfer-ential resection of the esophagus have always resulted in lethal strictures except when a stent has been used for at least 4 weeks (6)(7)(8).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Macrophage Phenotype Is Associated With the Regenerative Response in Experimental Replacement of the Porcine Esophagus

et al. 2015

View full text Add to dashboard Cite

A porcine model for bridging circumferential defects in the intrathoracic esophagus has been developed in order to improve the treatment of children born with long-gap esophageal atresia. The aim of this study was to identify factors beneficial for tissue regeneration in the bridging area in this model and to describe the histological progression 20 days after replacement with a silicone-stented Biodesign mesh. Resection of 3 cm of intrathoracic esophagus and replacement with a bridging graft was performed in six newly weaned piglets. They were fed through a gastrostomy for 10 days, and then had probe formula orally for another 10 days prior to sacrifice. Two out of six piglets had stent loss prior to sacrifice. In the four piglets with the stent in place, a tissue tube, with visible muscle in the wall, was seen at sacrifice. Histology showed that the wall of the healing area was well organized with layers of inflammatory cells, in-growing vessels, and smooth muscle cells. CD163+ macrophages was seen toward the esophageal lumen. In the animals where the stent was lost, the bridging area was narrow, and histology showed a less organized structure in the bridging area without the presence of CD163+ macrophages. This study indicates that regenerative healing was seen in the porcine esophagus 20 days after replacement of a part of the intrathoracic esophagus with a silicone-stented Biodesign mesh, if the bridging graft is retained. If the graft is lost, the inflammatory pattern changes with invasion of proinflammatory, M1 macrophages in the entire wall, which seems to redirect the healing process toward scar formation.

show abstract

Bioengineering of Trachea and Esophagus

Shibuya

Durkin

Garrido

et al. 2021

Organ Tissue Engineering

View full text Add to dashboard Cite

Piglet Model for Studying Esophageal Regrowth after Resection and Interposition of a Silicone Stented Small Intestinal Submucosa Tube

Cited by 14 publications

References 41 publications

Early Regenerative Response in the Intrathoracic Porcine Esophagus—The Impact of the Inflammation

Early Regenerative Response in the Intrathoracic Porcine Esophagus—The Impact of the Inflammation

Macrophage Phenotype Is Associated With the Regenerative Response in Experimental Replacement of the Porcine Esophagus

Bioengineering of Trachea and Esophagus

Contact Info

Product

Resources

About