Pigeon metallothionein consists of two species

Evolution of avian metallothionein: DNA sequence analyses of the Turkey metallothionein gene and metallothionein cDNAs from pheasant and quail

“…Southern blot analyses suggest the presence of one MT gene in pheasant, quail, turkey (Andrews and Fernando 1991), and duck (Lin and Huang 1990), but no analyses of other avian MT genes or cDNAs have been reported. The amino acid sequence of duck (Lin and Huang 1990;Lin et al 1990a) and pigeon (Lin et al 1990b) MTs and an amino acid composition for Japanese quail MT have been reported (Yamamura and Suzuki 1984). Comparisons of the amino acid sequences of duck and chicken MTs led to the remarkable finding that these avian proteins have an identical amino acid sequence (Lin et al 1990a).…”

“…It is possible that the quail expresses two MT genes in the zinc-treated liver, but expression of one gene (Q~) predominates. This has been documented to occur in the pigeon (Lin et al 1990b). The possibility should also be entertained that the base change found in the Qb cDNA clone reflects an error introduced by reverse transcriptase or more likely by Taq DNA polymerase during the RT-PCR procedures.…”

“…However, this is not a universal phenomenon among the birds. Amino acid sequence analysis of pigeon MTs established that the major isoform exhibited a high degree of amino acid sequence identity (91%), but was not identical with these avian MTs (Lin et al 1990b). Furthermore, the minor pigeon MT was quite divergent (75% identity) (Lin et al 1990b).…”

“…Amino acid sequence analysis of pigeon MTs established that the major isoform exhibited a high degree of amino acid sequence identity (91%), but was not identical with these avian MTs (Lin et al 1990b). Furthermore, the minor pigeon MT was quite divergent (75% identity) (Lin et al 1990b). Two MT genes are expressed in the mouse, and the proteins they encode have diverged significantly (79% identity in amino acids 15-57) (Searle et al 1984).…”

Purification, cDNA cloning and expression of a cadmium-inducible cysteine-rich metallothionein-like protein from the marine sponge Suberites domuncula

“…However, already after an incubation of 1 d in the presence of cadmium the expression of SDMTL increased strongly Rooted phylogenetic tree, constructed after alignment of the 4 sequences denoted under Fig. 4 together with the protostomian sequence from Caenorhabdtis elegans (9130l_CAEEL, CAA91301 [Wilson et al 1994]), crab, the MT 20-11 from the mussels MytiZus edulis (MT22_MYTED, P80252 [Mackay et al 1993]), and Dreissena polymorpha (MT-DREPO, Q94550, [Scekan et al 19961) and the oyster, the deuterostomian MTs from the carp Cyprinus carpio (MT-CARP, AAB61577 [Chan & Chan 1997]), the frog Xenopus laevis (MT-XENLA, Q05890 [Saint-Jacques & Seguin 1993]), the pigeon type-I (MT1-COLLI, 'P15786 (Lin et al 1990]), human type-IV (MT4_HUMAN, P47944 [Quaife et al 1994]), mouse type-IV (MT4_MOUSE, P47945 [Quaife et al 1994]), bovine type-I1 (MT2BOVIN, P09579 [Munger et al 1985)) and from horse as well as from the sea urchin Sterechinus neumayeri (MT-STENE, P55953 [Scudiero et al 19971). In addition, the MT (related) sequences from the fungus Candida glabrata (MT1-CANGA, P15113 [Mehra et al 19891) and the cysteinerich protein CRPl from the earthworm Enchytraeus buchholzi (CRP1-WORM, A55035 [Willuhn et al 19941) have been included.…”