Pig standard bivariate flow karyotype and peak assignment for chromosomes X, Y, 3, and 7

Schmitz, Annette; Chardon, Patrick; Gainche, Isabelle; Chaput, Brigitte; Guilly, Marie-Noëlle; Frelat, G.; Vaiman, M.

doi:10.1016/s0888-7543(05)80226-5

Cited by 20 publications

(13 citation statements)

References 17 publications

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“…The assignments of chromosomes 3 and 7 to peaks F and G have been established previously by polymerase chain reaction (PCR) on sorted chromosomes and study of translocated t(3,7) pigs. Chromosomes 1 and 13 were assigned to peaks A and B, and chromosomes X and Y to peaks H and Y, respectively (15). These results were incorporated into the peak content estimation we propose.…”

Section: Discussionmentioning

confidence: 99%

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Swine chromosomal DNA quantification by bivariate flow karyotyping and karyotype interpretation

et al. 1992

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Human and swine chromosomes were analyzed separately and as a mix to obtain bivariate flow karyotypes. They were normalized to each other in order to use the human chromosomal DNA content as standard. Our results led to the characterization of the "DNA line" in swine identical to the human "DNA line." Estimation of the DNA content in megabase pairs of the swine chromosomes is proposed. Chromosomal assignment to the various resolved peaks on the bivariate swine flow karyotype is suggested from the relation between DNA content quantified by flow cytometry and chromosomal size. Swine chromosomes 1,13, 6,5,10,16,11,18, and Y were assigned to peaks A, B, C, K, L, N, 0, Q, and Y, respectively. Peaks D and E were assumed to contain chromosomes 2 and 14, but without specific assignment. Similarly, P and M peaks were expected to correspond to chromosomes 12 and 17. Of the remaining chromosomes (3, 7, X, 8, 15, 9, and 4), chromosomes 3, 7, and X, which were assigned previously to peaks F, G, and H, respectively, led us to deduce that chromosomes 15 and 8 belonged to peaks I and J, and chromosomes 9,4, and X to peak H. CI 1992 Wiley-Liss, Inc.Key terms: DNA line, chromosome identification Flow cytogenetic techniques already contribute greatly to our knowledge of the genome in mammals. They have, for instance, proved invaluable in detecting numeral and structural aberrations associated with human genetic diseases. They have also been extensively applied to human chromosome sorting, thereby permitting the construction of chromosome-specific DNA libraries (see ref. 6 for review). Applied to domestic species, flow cytometric techniques have been particularly straightforward in swine cytogenetic analyses, as most of the chromosomes in this species are both fairly different in size, and are fewer in number compared with human or bovine chromosomes (22).Flow cytogenetics and chromosome sorting therefore appear especially relevant for the ongoing European PiGMaP project (2). Among the aims of the project is the construction of chromosome-specific libraries, in order to provide as quickly as possible chromosome-assigned markers, which in conjunction with other techniques will allow coverage of the whole genome. A prerequisite to production of chromosome-specific libraries is accurate assignment of all, or at least most, of the swine chromosomes to the peaks of the flow karyotype and the estimation of the swine chromosomal DNA content. We therefore further developed studies on bivariate flow karyotypes in swine, as previous results showed that good chromosome resolution was easily achievable (7,s). All swine chromosome types could be resolved into individual peaks except two autosomal pairs plus chromosome X, which were confounded (15).The work reported here concerns the establishment of a "DNA line" in swine, a technique successfully applied to human chromosomes (17,3,18 somes to the various peaks on the bivariate flow karyotype. MATERIALS AND METHODSAnimals We analyzed the karyotypes of 11 pigs of both sexes, 9 males and 2 females...

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Section: Discussionmentioning

confidence: 99%

“…We therefore further developed studies on bivariate flow karyotypes in swine, as previous results showed that good chromosome resolution was easily achievable (7,s). All swine chromosome types could be resolved into individual peaks except two autosomal pairs plus chromosome X, which were confounded (15).…”

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Swine chromosomal DNA quantification by bivariate flow karyotyping and karyotype interpretation

et al. 1992

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“…For chromosomes 3, 12 and 14, the probes were produced from flow-sorted chromosomes amplified using priming authorizing random mismatches (PARM) -PCR (Schmitz et al, 1992;Yerle et al, 1993). The probe for chromosome 15 was produced by chromosomal microdissection followed by degenerate oligonucleotide-primed (DOP) -PCR amplification (Telenius et al, 1992) of the microdissected material (Pinton et al, 2003).…”

Section: In Vitro Maturation Of Oocytesmentioning

confidence: 99%

Comparison of male and female meiotic segregation patterns in translocation heterozygotes: a case study in an animal model (Sus scrofa domestica L.)

Pinton¹,

Faraut²,

Yerle³

et al. 2005

Human Reproduction

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BACKGROUND: The comparison of male and female meiotic segregation patterns for individuals carrying identical reciprocal translocations has been rarely reported in mammalian species. The main comparative study involving males and females with comparable genetic background has been performed in the mouse. Swine is another relevant animal model species for meiotic studies. Here we present the segregation patterns determined for sows carrying one of the two following reciprocal translocations: 38, XX, rcp(3;15)(q27;q13), and 38, XX, rcp(12;14)(q13;q21). These segregation data were compared to those previously obtained for closely related boars carrying the same balanced chromosomal rearrangements. METHODS: Dual colour in situ hybridization of whole chromosome painting probes was carried out on metaphases of in vitro-matured oocytes II. Segregation results were obtained for 118 and 206 metaphases II respectively for the two translocations. RESULTS: Significant differences between sexes were demonstrated for both rearrangements. For instance, for the 3/15 translocation, the chromosomally unbalanced gametes were of different origin: preponderance of the adjacent-I segregation in the male (31.4%), and of the adjacent-II (14.3%) and 3:1 (14.3%) segregations in females. For the 12/14 translocation, the proportion of balanced gametes was greater in males than in females (75.9 and 59.4% respectively). CONCLUSION: This study is a new scientific contribution to compare the segregation patterns of male and female carriers of identical chromosomal rearrangements. The results obtained are consistent with those previously reported in mice. Hypotheses to interpret the observed differences between the two translocations, as well as between the male and female segregation patterns, are formulated and discussed.

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“…Dual-color chromosome painting was carried out, except for case 3 (no chromosomal material was kept), to confirm the hypotheses put forward as a result of the banding techniques. Chromosome-specific painting probes were produced using DOP-PCR amplification [31] of flow sorted L52 swine chromosomes [29,33]. Fluorescence in situ hybridization was performed according to [33].…”

Section: Cytogenetical Analysesmentioning

confidence: 99%

Cytogenetic and molecular characterization of eight new reciprocal translocations in the pig species. Estimation of their incidence in French populations

et al. 2002

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-Eight new cases of reciprocal translocation in the domestic pig are described. All the rearrangements were highlighted using GTG banding techniques. Chromosome painting experiments were also carried out to confirm the proposed hypotheses and to accurately locate the breakpoints. Three translocations, rcp(4;6)(q21;p14), rcp(2;6)(p17;q27) and rcp(5;17)(p12;q13) were found in boars siring small litters (8.3 and 7.4 piglets born alive per litter, on average, for translocations 2/6 and 5/17, respectively). The remaining five, rcp(5;8)(p12;q21), rcp(15;17)(q24;q21), rcp(7;8)(q24;p21), rcp(5;8)(p11;p23) and rcp(3;15)(q27;q13) were identified in young boars controlled before entering reproduction. A decrease in prolificacy of 22% was estimated for the 3/15 translocation after reproduction of the boar carrier. A parental origin by inheritance of the translocation was established for the (5;8)(p11;p23) translocation.

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Pig standard bivariate flow karyotype and peak assignment for chromosomes X, Y, 3, and 7

Cited by 20 publications

References 17 publications

Swine chromosomal DNA quantification by bivariate flow karyotyping and karyotype interpretation

Swine chromosomal DNA quantification by bivariate flow karyotyping and karyotype interpretation

Comparison of male and female meiotic segregation patterns in translocation heterozygotes: a case study in an animal model (Sus scrofa domestica L.)

Cytogenetic and molecular characterization of eight new reciprocal translocations in the pig species. Estimation of their incidence in French populations

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