Piezoelectric Poly(3-hydroxybutyrate)-Poly(lactic acid) Three Dimensional Scaffolds for Bone Tissue Engineering

Mendenhall, Juana; Li, Dapeng; Frey, Margaret W.; Hinestroza, Juan P.; Babalola, Omotunde M.; Bonnasar, Lawrence; Batt, Carl A.

doi:10.1557/proc-1025-b12-03

Cited by 9 publications

(5 citation statements)

References 17 publications

(11 reference statements)

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“…Finally, 3D complex polymeric piezoelectric scaffolds for bone tissue engineering have been recently fabricated using poly(3-hydroxybutyrate)-poly(lactic acid) [63]. Microfibres (~ 2.5 µm in diameter) were prepared via electrospinning using poly(lactic acid); subsequently, the electrospun meshes were coated with poly (3-hydroxybutyrate) by means of surface-initiated polymerisation.…”

Section: Bone Tissue Engineeringmentioning

confidence: 99%

Applications of Piezoelectricity in Nanomedicine

Ciofani

Danti

Ricotti

et al. 2012

Nanomedicine and Nanotoxicology

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Abstract. In this Chapter, recent results about studies of interactions between piezoelectric nanoparticles and living systems will be discussed. As extremely innovative materials, great importance is devoted to the investigations of their stabilisation in physiological environments and to their biocompatibility. Applications as drug carriers and nanovectors will be thereafter described, and special attention will be dedicated to tissue engineering applications. Finally, preliminary results achieved by our group on "wireless" cell stimulation will be approached. IntroductionNanotechnology refers to the research and technology development at atomic, molecular and macromolecular scales, which leads to the controlled manipulation and study of structures and devices with length scales in the range of 1-100 nm [1].In the latest two decades, the research on nanotechnology has grown explosively with over 300,000 publications in the field of nanosciences according to ISI Web of Science. Among these spectacular developments, a new emerging field that combines nanotechnology and biotechnology -nanobiotechnology -is receiving a growing attention [2]. Nanostructured materials own unique properties which are of great interest for their potential applications as biomaterials, such as the large "surface-area to volume" ratio. Since life itself, fundamentally, is a collection of nanoscale processes occurring within cells [3], it is unavoidable and necessary to understand the impacts of the presence of nanomaterials inside the cells when one explores advantages and promises of nanomaterials for biomedical applications.In fact, it has been demonstrated that nanomaterials can interact both positively and negatively with different biological systems. Carbon nanotubes (CNTs), for

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Section: Bone Tissue Engineeringmentioning

confidence: 99%

Applications of Piezoelectricity in Nanomedicine

Ciofani

Danti

Ricotti

et al. 2012

Nanomedicine and Nanotoxicology

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“…The glass transition temperature, T g , remains constant for PHB for different thickness . The lamellar orientation and stability of crystals are dependent on the interfacial boundary and the film thickness. − For example, a crystal structure of PHB with larger (020) crystal plane distance of α-form exists near the free surface of the film which transforms into the highly ordered α-form during heating process . PHB is used in the field of regenerative medicine and tissue engineering, such as nerve guides and artificial esophagus .…”

Section: Introductionmentioning

confidence: 99%

“…17 PHB is used in the field of regenerative medicine and tissue engineering, such as nerve guides and artificial esophagus. 18 Also, it is frequently used as thin film (2D) or is found in nature, that is, in the cell, as a nanoparticle (0D). Consequently, understanding the kinetics of crystallization and the dynamics of the polymer chain under these different confinement conditions can have significant impact.…”

Section: ■ Introductionmentioning

confidence: 99%

Confinement Effects on the Crystallization of Poly(3-hydroxybutyrate)

Dai

Ren

et al. 2018

Macromolecules

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Poly(3-hydroxybutyrate) (PHB) is taken as an example to explore (i) whether the confined crystallization occurring in anodized aluminum oxide (AAO) nanopores is the same as that in ultrathin films, and (ii) whether the interfacial effect of curve surface is the same as flat surface. The crystallization behavior of PHB in AAO and thin films (sandwiched between two plates) has been compared. A curvature-dependent crystallization behavior of PHB is identified. Stable intermediate structures of PHB confined in narrow pores (diameter <100 nm), which have never been observed in bulk, are obtained for the first time. In larger pores (pore diameter >100 nm), crystallization occurs both in the center and interfacial regions in contact with AAO inner wall. This implies that the strength of interfacial layer weakens with decreased curvature and has been further proved by the crystallization behavior of its sandwiched ultrathin film. It is found that the highly restricted interface layer incapable of crystallization is about 30 nm for film, which is much thinner than that for nanorods (approximately 100 nm). We have also identified two different relaxations of PHB nanorods corresponding to the interfacial effect and spatial confinement, respectively. While the relaxation correlated to the interfacial effect with a slower relaxation time strengthens, the relaxation corresponding to spatial confinement with a faster relaxation time (bulklike α-relaxation) weakens with decreased pore size. This is completely different from that of sandwiched thin film, where only a thickness-independent α-relaxation same as bulk is observed 1 (Macromolecules2006395967). Therefore, the reduced crystallization kinetics of thin film is attributed to the reduction of long-range chain mobility. By contrast, the inhibited crystallization of PHB nanorods in AAO is attributed to the reduced segmental dynamics and the reduced chain mobility of PHB layer at interface.

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“…Over time, the fibers create a mesh resembling a 3D tissue scaffold. Our previous work consisted of preparing 3D electrospun fiber surfaces functionalized with biomaterials such as poly(hydroxybutyrate)[PHB] . These 3D scaffolds formed a matrix for bone cells that support piezoelectric activity due to the carbonyl (CO) interface in the scaffold.…”

Section: Resultsmentioning

confidence: 99%

“… Piezoelectric Poly(3-hydroxybutryate)-Poly(lactic acid) Three Dimensional Scaffolds for Bone Tissue EngineeringMendenhallJ.HinestrozaJ.FreyM.BarbellO.BonnasarL.BattC. Mendenhall, J. Hinestroza, J. Frey, M. Barbell, O. Bonnasar, L. Batt, C. MRS Symposium Proceedings Series: Nanoscale Phenomena in Functional Materials by Scanning Probe Microscopy20071025E . The preparation of poly(lactic acid) electrospun fibers functionalized with poly(hydroxybutryare) was presented along with a biocompatibility study to support bone tissue scaffolding. Tunable thermo-responsive poly (N-vinylcaprolactam) cellulose nanofibers: synthesis, characterization, and fabricationWebsterM.MiaoJ.LynchB.GreenD.…”

Section: Key Referencesmentioning

confidence: 99%

Three-Dimensional Biomaterials with Spatiotemporal Control for Regenerative Tissue Engineering

Mendenhall

2023

Acc. Chem. Res.

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Metrics & MoreArticle Recommendations CONSPECTUS: At Morehouse College, one of the nation's top liberal arts historically black colleges and universities (HBCU) for African American men, research experiences are used to enhance the liberal arts educational experience. Securing research funding to train HBCU students is highly competitive and challenging due to the review process that is typically vetted by scientists from research-intensive universities who may not be familiar with the HBCU enterprise that may be comprised of insolvent infrastructures. In this Account, the synthesis and preparation of synthetic polymeric biomaterials that are used to facilitate or support changes in biological processes, enhance mechanical properties, and foster tissue growth in three dimensions (3D) under disease conditions will be discussed. The use of biomaterials to help control biological processes in disease states is limited.Hence, the fabrication of 3D scaffolds with chemical variability to grow or repair damaged tissues by inhibiting molecular pathways shows promise by controlling the cellular response to recapitulate 3D tissues and organs. The Mendenhall laboratory at Morehouse College uses 3D biomaterials to solve biological problems by probing cellular mechanistic pathways using natural products and nanoparticles. Toward this end, we have fabricated and manufactured 3D biomaterial scaffolds using chemical strategies to mitigate biological processes to help restore pristine tissue properties. Hydrogels are 3D polymeric matrixes that swell in aqueous environments and support cell growth that later infuriates the 3D matrix to create new tissue(s). In contrast, electrospun fibers use high electric fields to create porous 3D polymeric structures that can be used to create 3D tissue molds. The synergistic use of 3D biomaterial templates that can inhibit cellular damage while providing a mechanically strong scaffold to support regenerative tissue growth is essential to creating the next generation of biomaterials. This approach will require foresight using tools from synthetic biology, molecular biology, autonomous processes, advanced biomanufacturing, and machine learning (ML). The use of several biomaterials has been explored by the Mendenhall laboratory to design, prepare, fabricate, characterize, and evaluate 3D electrospun fibers and hydrogels containing hybrid compositions of polylactic acid (PLA), poly(n-vinylcaprolactam) (PVCL), cellulose acetate (CA), and methacrylated hyaluronic acid (meHA). This work contributed to the newly fabricated PVCL-CA fibers with morphological changes and nanoscale fiber hydrophobic surface properties. While the use of electrospun fibers can create hierarchal scaffolds for bone tissue engineering, the use of injectable gels for nonporous tissues such as articular cartilage presents another compelling biomaterial challenge. Using graft polymerization, we prepared PVLC-graft-HA and studied the effect of lower critical solution temperatures (LCSTs), gelation temperatures, and mechanical prop...

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Piezoelectric Poly(3-hydroxybutyrate)-Poly(lactic acid) Three Dimensional Scaffolds for Bone Tissue Engineering

Cited by 9 publications

References 17 publications

Applications of Piezoelectricity in Nanomedicine

Applications of Piezoelectricity in Nanomedicine

Confinement Effects on the Crystallization of Poly(3-hydroxybutyrate)

Three-Dimensional Biomaterials with Spatiotemporal Control for Regenerative Tissue Engineering

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