Piezoelectric-based biosensors

Walton, Philip W.; Butler, Mark E.; O'Flaherty, M.R.

doi:10.1042/bst0190044

Cited by 17 publications

(3 citation statements)

References 4 publications

(6 reference statements)

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“…Buoyant force has been suggested as a factor affecting the QCM's signal in solutions, but one cannot attribute a direct effect to buoyant force because the QCM is insensitive to gravity and responds only to inertia. It is conceivable that buoyant force, if it has any effect at all, might play a role in establishing a tethered macromolecule's average position relative to the QCM surface, but no experimental investigations of this factor have been published.…”

Section: Resultsmentioning

confidence: 99%

QCM Response to Solvated, Tethered Macromolecules

et al. 1998

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When the quartz crystal microbalance (QCM) is operated in contact with solution and used to detect inertia increases caused by macromolecules binding to its surface, resonance frequency shifts are reported in the literature to be greater than, less than, and the same as an identical macromolecular mass would cause as a dry layer. A previous report of wet and dry M13 DNA giving the same, linear frequency versus mass response is examined. The M13 data are shown to follow the reciprocal of the square root of mass, not the reported linear relationship. New experiments on RNA duplexes oscillated in solution are reported. A lossy polymer layer is placed between the QCM and RNA. When changes in density, viscosity, and included water are eliminated, the response remains linear for a constant adlayer thickness. The expectation that response per unit mass should decrease with distance from the QCM surface is demonstrated. Total decoupling of mass lying beyond the acoustic overlayer is also demonstrated. The present results are placed in context with recently published results from a study of progressively thicker protein layers bound to the QCM.

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Section: Resultsmentioning

confidence: 99%

QCM Response to Solvated, Tethered Macromolecules

et al. 1998

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“…7 The mass sensitivity of the QCM has been exploited in both the vapour phase and in liquids, and there are numerous reported applications. [1][2][3][4][5][8][9][10][11][12][13][14][15][16] Two areas of the application of QCM devices are of immediate interest. The first involves the monitoring of biomolecular reactions in the liquid phase, in particular kinetic immunoassays which can be followed by the change in frequency as the mass on the surface of the crystal increases.…”

mentioning

confidence: 99%

“…[1][2][3][4][5] Application of an electric field across the crystal, using an electronic oscillator, results in vibration of the crystal at its resonant frequency. The frequency of the oscillating crystal is decreased by deposition of mass on its surface and it has been shown that in the gas phase the change in frequency for a quartz crystal is related to change in mass by the Sauerbrey equation.…”

mentioning

confidence: 99%

Detection of lead(II) and silver(I) in aqueous solution using a piezoelectric device

Cox¹,

Hill²,

Gahan³

1999

Analyst

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The gold surface of a quartz crystal microbalance was modified by the attachment of silica particles derivatised with N- [(3-trimethoxysilyl)propyl]ethylenediaminetriacetic acid. The device was employed to study the kinetics of the interaction of aqueous solutions of lead(ii) nitrate and silver(i) nitrate with the surface and for the selective separation of the metal ions.The quartz crystal microbalance (QCM) consists of a thin, circular, quartz crystal with circular gold electrodes placed centrosymmetrically on opposite faces. 1-5 Application of an electric field across the crystal, using an electronic oscillator, results in vibration of the crystal at its resonant frequency. The frequency of the oscillating crystal is decreased by deposition of mass on its surface and it has been shown that in the gas phase the change in frequency for a quartz crystal is related to change in mass by the Sauerbrey equation. 6 These devices are radially sensitive, i.e., it is the area under the gold electrode surface which is the most sensitive part, and the quartz surface projecting radially from the centre becomes less sensitive to mass deposited. 7 The mass sensitivity of the QCM has been exploited in both the vapour phase and in liquids, and there are numerous reported applications. [1][2][3][4][5][8][9][10][11][12][13][14][15][16] Two areas of the application of QCM devices are of immediate interest. The first involves the monitoring of biomolecular reactions in the liquid phase, in particular kinetic immunoassays which can be followed by the change in frequency as the mass on the surface of the crystal increases. 17 The piezoelectric device offers an alternative to the measurement of reaction kinetics of heterogeneous systems involving biomacromolecular interactions using the BIAcore TM instrument, although in the latter case the kinetic analyses have been more extensively investigated and developed. 18-20 Second, we are interested in the development of piezoelectric devices for the detection and determination of metal ions in solution, 14,[21][22][23][24][25][26][27] where sorbents chemically attached to the crystal are employed in order to obtain the selectivity required to determine one metal ion in the presence of others.We describe an application of the QCM which combines the two areas. The QCM device described in this work is not employed specifically as a mass sensor, in line with recent studies which question this aspect of their use, 28,29 but more so as an event sensor. A QCM device, derivatised with an aminopolycarboxylate ligand covalently attached to spherical silica particles, is employed in the search for selective substrates and/or conditions under which it would be possible to separate metal ions under flow conditions. A combination of the determination of an adsorption isotherm and investigation of association and dissociation phase kinetic parameters has been employed to permit the prediction of the behaviour of two metal ions on the derivatised silica substrate.

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Spezifische Bindung einer funktionellen Proteinschicht an eine trägerfixierte Streptavidinmatrix

et al. 1992

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Einen langen, flexiblen, hydrophilen Spacer muß das an einer Lipidmischmembran beteiligte Biotinlipid aufweisen, um eine spezifische Bindung des Proteins Streptavidin an diese Mischmembran zu erm&öuml;glichen. Dies ergaben Messungen mit deiner Quarzmikrowaage, bei denen sich 1 als spezifisch bindend erwies. Mit diesem Verfahren konnte auch das Binden einer zweiten Proteinschicht aus biotinyliertem Fab‐Fragment an die Streptavidinmatrix in Echtzeit verfolgt werden

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Piezoelectric-based biosensors

Cited by 17 publications

References 4 publications

QCM Response to Solvated, Tethered Macromolecules

QCM Response to Solvated, Tethered Macromolecules

Detection of lead(II) and silver(I) in aqueous solution using a piezoelectric device

Spezifische Bindung einer funktionellen Proteinschicht an eine trägerfixierte Streptavidinmatrix

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