Piezo Proteins: Regulators of Mechanosensation and Other Cellular Processes

Bagriantsev, Sviatoslav N.; Gracheva, Elena O.; Gallagher, Patrick G.

doi:10.1074/jbc.r114.612697

Cited by 194 publications

(181 citation statements)

References 75 publications

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“…One possible pathway for Ca 2+ entry is the mechanosensitive channels in the RBC membrane [48]. RBCs do become less deformable, eventually, because of an increase in cytoplasmic viscosity due to dehydration and increase in Hb concentration.…”

Section: Discussionmentioning

confidence: 99%

Membrane Remodelling and Vesicle Formation During Ageing of Human Red Blood Cells

Ciana

Achilli

Gaur

et al. 2017

Cell Physiol Biochem

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Background/Aims: A high surface-to-volume ratio and a spectrin membrane-skeleton (MS) confer to the mammalian red blood cells (RBCs) their characteristic deformability, mechanical strength and structural stability. During their 120 days of circulatory life in humans, RBCs decrease in size, while remaining biconcave disks, owing to a coordinated decrease in membrane surface area and cell water. It is generally believed that part of the membrane is lost with the shedding of spectrin-free vesicles of the same type that can be obtained in vitro by different treatments. If this were true, an excess of MS would arise in old RBCs, with respect to the lipid bilayer. Aim of this paper was to investigate this aspect. Methods: Quantification of spectrin by electrophoretic methods was carried out in RBCs of different age. Results: Spectrin decreases, on a per cell basis, with RBC ageing. On the other hand, the membrane raft protein marker flotillin-2, while decreasing in the membrane of old cells, was found to be strongly depleted in the membrane of in vitro-induced vesicles. Conclusion: Part of the membrane-skeleton is probably lost together with part of the lipid bilayer in a balanced way. These findings point to a mechanism for the in vivo release of membrane that is different from that which is known to occur in vitro.

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Section: Discussionmentioning

confidence: 99%

Membrane Remodelling and Vesicle Formation During Ageing of Human Red Blood Cells

Ciana

Achilli

Gaur

et al. 2017

Cell Physiol Biochem

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“…Molecular studies have identified a diverse set of ion channels in the nervous system that have the capacity to detect and respond to mechanical and thermal cues. Members of the K 2P potassium channel family (Honoré, 2007; Nilius and Honoré, 2012; Noël et al, 2011), transient receptor potential (TRP) family (Anishkin et al, 2014; Julius, 2013; Kung, 2005; Vay et al, 2012; Vriens et al, 2014), and newly discovered Piezo channels (Bagriantsev et al, 2014; Nilius and Honoré, 2012) are thought to possess intrinsic mechanisms through which they detect and respond to pressure changes, temperature changes, or both. Although structural data have begun to become available for these families (Brohawn et al, 2012, 2013; Cao et al, 2013; Kamajaya et al, 2014; Liao et al, 2013), the molecular mechanisms by which such channels can detect and sense changes in pressure and temperature remain incompletely understood.…”

Section: Introductionmentioning

confidence: 99%

Transmembrane Helix Straightening and Buckling Underlies Activation of Mechanosensitive and Thermosensitive K2P Channels

Lolicato

Riegelhaupt

Arrigoni

et al. 2014

Neuron

117

192

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SUMMARY Mechanical and thermal activation of ion channels is central to touch, thermosensation, and pain. The TRAAK/TREK K2P potassium channel subfamily produces background currents that alter neuronal excitability in response to pressure, temperature, signaling lipids, and anesthetics. How such diverse stimuli control channel function is unclear. Here we report structures of K2P4.1 (TRAAK) bearing C-type gate-activating mutations that reveal a tilting and straightening of the M4 inner transmembrane helix and a buckling of the M2 transmembrane helix. These conformational changes move M4 in a direction opposite to that in classical potassium channel activation mechanisms and open a passage lateral to the pore that faces the lipid bilayer inner leaflet. Together, our findings uncover a unique aspect of K2P modulation, indicate a means for how the K2P C-terminal cytoplasmic domain affects the C-type gate which lies ~40Å away, and suggest how lipids and bilayer inner leaflet deformations may gate the channel.

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“…Most of our knowledge of the physiology and molecular basis of mechanosensation comes from studies with rodents, worms, and flies, and is extensively covered in recent reviews (3,32,38,41,59,72,78,80,82,84). Here, we will focus on adaptations to tactile perception in acutely mechanosensitive vertebrates, which are not considered standard model organisms.…”

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confidence: 98%

Evolutionary Specialization of Tactile Perception in Vertebrates

2016

Self Cite

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Evolution has endowed vertebrates with the remarkable tactile ability to explore the world through the perception of physical force. Yet the sense of touch remains one of the least well understood senses at the cellular and molecular level. Vertebrates specializing in tactile perception can highlight general principles of mechanotransduction. Here, we review cellular and molecular adaptations that underlie the sense of touch in typical and acutely mechanosensitive vertebrates.

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Piezo Proteins: Regulators of Mechanosensation and Other Cellular Processes

Cited by 194 publications

References 75 publications

Membrane Remodelling and Vesicle Formation During Ageing of Human Red Blood Cells

Membrane Remodelling and Vesicle Formation During Ageing of Human Red Blood Cells

Transmembrane Helix Straightening and Buckling Underlies Activation of Mechanosensitive and Thermosensitive K2P Channels

Evolutionary Specialization of Tactile Perception in Vertebrates

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