Pien Tze Huang, a Composite Chinese Traditional Herbal Extract, Affects Survival of Neuroblastoma Cells

Lü, Lei; Wai, Maria Sen Mun; Yew, David T.; Mak, Y. T.

doi:10.1080/00207450802324770

Cited by 12 publications

(5 citation statements)

References 13 publications

(14 reference statements)

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“…Besides the inflammation in the CNS, demyelination is also the typical characteristics in MS. Interestingly, several studies have indicated that PZH had a neuroprotective effect [ 40 , 41 ], which makes us speculate whether it also exerts the similar effect in EAE rats. Unfortunately, due to the slight demyelination in an MBP-induced rat model [ 20 ], we did not find marked remyelination in PZH-treated EAE rats (data not shown).…”

Section: Discussionmentioning

confidence: 99%

Therapeutic Potential of Pien Tze Huang on Experimental Autoimmune Encephalomyelitis Rat

Qiu

Hui

et al. 2018

Journal of Immunology Research

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Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease of the central nervous system (CNS). There is still lack of commercially viable treatment currently. Pien Tze Huang (PZH), a traditional Chinese medicine, has been proved to have anti-inflammatory, neuroprotective, and immunoregulatory effects. This study investigated the possible therapeutic effects of PZH on experimental autoimmune encephalomyelitis (EAE) rats, a classic animal model of MS. Male Lewis rats were immunized with myelin basic protein (MBP) peptide to establish an EAE model and then treated with three doses of PZH. Clinical symptoms, organ coefficient, histopathological features, levels of proinflammatory cytokines, and chemokines as well as MBP and Olig2 were analyzed. The results indicated that PZH ameliorated the clinical severity of EAE rats. It also remarkably reduced inflammatory cell infiltration in the CNS of EAE rats. Furthermore, the levels of IL-17A, IL-23, CCL3, and CCL5 in serum and the CNS were significantly decreased; the p-P65 and p-STAT3 levels were also downregulated in the CNS, while MBP and Olig2 in the CNS of EAE rats had a distinct improvement after PZH treatment. In addition, PZH has no obvious toxicity at the concentration of 0.486 g/kg/d. This study demonstrated that PZH could be used to treat MS.

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Section: Discussionmentioning

confidence: 99%

Therapeutic Potential of Pien Tze Huang on Experimental Autoimmune Encephalomyelitis Rat

Qiu

Hui

et al. 2018

Journal of Immunology Research

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“…Our previous studies found that PZH protected the liver from carbon tetrachloride damage [20] and that PZH showed anti-cancer activities [21]. Our studies on Gingko suggested the possible general molecular mechanisms of neuroprotective effects [6,22,23], including apoptosis, oxidative stress, free radical in the brain cells.…”

Section: Introductionmentioning

confidence: 78%

Protective effects and potential mechanisms of Pien Tze Huang on cerebral chronic ischemia and hypertensive stroke

et al. 2010

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BackgroundStroke caused by brain ischemia is the third leading cause of adult disability. Active prevention and early treatment of stroke targeting the causes and risk factors may decrease its incidence, mortality and subsequent disability. Pien Tze Huang (PZH), a Chinese medicine formula, was found to have anti-edema, anti-inflammatory and anti-thrombotic effects that can prevent brain damage. This study aims to investigate the potential mechanisms of the preventive effects of Pien Tze Huang on brain damage caused by chronic ischemia and hypertensive stroke in rats.MethodsThe effects of Pien Tze Huang on brain protein expression in spontaneously hypertensive rat (SHR) and stroke prone SHR (SHRsp) were studied with 2-D gel electrophoresis and mass spectrometric analysis with a matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF)/TOF tandem mass spectrometer and on brain cell death with enzyme link immunosorbent assay (ELISA) and immunostaining.ResultsPien Tze Huang decreased cell death in hippocampus and cerebellum caused by chronic ischemia and hypertensive stroke. Immunostaining of caspase-3 results indicated that Pien Tze Huang prevents brain cells from apoptosis caused by ischemia. Brain protein expression results suggested that Pien Tze Huang downregulated QCR2 in the electron transfer chain of mitochondria preventing reactive oxygen species (ROS) damage and possibly subsequent cell death (caspase 3 assay) as caused by chronic ischemia or hypertensive stroke to hippocampus and cerebellum.ConclusionPien Tze Huang showed preventive effects on limiting the damage or injury caused by chronic ischemia and hypertensive stroke in rats. The effect of Pien Tze Huang was possibly related to prevention of cell death from apoptosis or ROS/oxidative damage in mitochondria.

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“…The anti-proliferative activities of PTH in vitro are summarized in Table 1. A previous study showed that PTH can dose-dependently inhibit the survival of neuroblastoma SH-SY5Y cells without affecting normal fibroblast NIH-3T3 cells [54]. PTH also inhibits the viability of human ovarian cancer OVCAR-3 cell line by inducing G1/S-phase cell arrest without inducing apoptosis.…”

Section: Pharmacologymentioning

confidence: 99%

Therapeutic Potential of Pien-Tze-Huang: A Review on Its Chemical Composition, Pharmacology, and Clinical Application

Huang

Zhang

et al. 2019

Molecules

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Pien-Tze-Huang (PTH) is a famous and commonly used traditional Chinese medicine formula in China. It was first formulated by a royal physician of the Ming Dynasty (around 1555 AD). Recently, PTH has attracted attention worldwide due to its beneficial effects against various diseases, especially cancer. This paper systematically reviewed the up-to-date information on its chemical composition, pharmacology, and clinical application. A range of chemical compounds, mainly ginsenosides and bile acids, have been identified and quantified from PTH. Pharmacological studies indicated that PTH has beneficial effects against various cancers, hepatopathy, and ischemic stroke. Furthermore, PTH has been used clinically to treat various diseases in China, such as colorectal cancer, liver cancer, and hepatitis. In summary, PTH is a potential agent with extensive therapeutic effects for the treatment of various diseases. However, the lack of information on the side effects and toxicity of PTH is a non-negligible issue, which needs to be seriously studied in the future.

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Pien Tze Huang, a Composite Chinese Traditional Herbal Extract, Affects Survival of Neuroblastoma Cells

Cited by 12 publications

References 13 publications

Therapeutic Potential of Pien Tze Huang on Experimental Autoimmune Encephalomyelitis Rat

Therapeutic Potential of Pien Tze Huang on Experimental Autoimmune Encephalomyelitis Rat

Protective effects and potential mechanisms of Pien Tze Huang on cerebral chronic ischemia and hypertensive stroke

Therapeutic Potential of Pien-Tze-Huang: A Review on Its Chemical Composition, Pharmacology, and Clinical Application

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