2003
DOI: 10.1091/mbc.e02-08-0483
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Piecemeal Microautophagy of Nucleus inSaccharomyces cerevisiae

Abstract: Nucleus-vacuole (NV) junctions in Saccharomyces cerevisiae are formed through specific interactions between Vac8p on the vacuole membrane and Nvj1p in the nuclear envelope. Herein, we report that NV junctions in yeast promote piecemeal microautophagy of the nucleus (PMN). During PMN, teardrop-like blebs are pinched from the nucleus, released into the vacuole lumen, and degraded by soluble hydrolases. PMN occurs in rapidly dividing cells but is induced to higher levels by carbon and nitrogen starvation and is u… Show more

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Cited by 421 publications
(503 citation statements)
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“…In yeast, contact sites between the nucleus and vacuole, termed nucleus-vacuole junctions (NVJ), form by the association of the vacuolar protein Vac8 and the ER-resident protein Nvj1 ( Figure 3C). NVJ formation is necessary for piecemeal microautopaghy of the nucleus (PMN), which can be induced upon carbon or nitrogen starvation (Roberts et al , 2003 ). The sterol-binding protein Osh1 and fatty acid elongase Tsc13 localize to the NVJ.…”
Section: Organellar Contact Zones As Novel Microcompartmentsmentioning
confidence: 99%
“…In yeast, contact sites between the nucleus and vacuole, termed nucleus-vacuole junctions (NVJ), form by the association of the vacuolar protein Vac8 and the ER-resident protein Nvj1 ( Figure 3C). NVJ formation is necessary for piecemeal microautopaghy of the nucleus (PMN), which can be induced upon carbon or nitrogen starvation (Roberts et al , 2003 ). The sterol-binding protein Osh1 and fatty acid elongase Tsc13 localize to the NVJ.…”
Section: Organellar Contact Zones As Novel Microcompartmentsmentioning
confidence: 99%
“…[9][10][11] A degradation of portions of the nucleus, termed Piecemeal Microautophagy of the Nucleus (PMN), has been reported in the yeast Saccharomyces cerevisiae submitted to nitrogen starvation. 12 PMN involves a direct contact between the vacuole and the nucleus, the formation of junctions, and the protrusion of parts of the nucleus inside the vacuole, forming vesicles that are later degraded. The formation of these junctions has been shown to involve a protein-protein interaction between Nvj1p 13 on the nuclear envelope and Vac8p on the vacuolar membrane.…”
Section: Introductionmentioning
confidence: 99%
“…The formation of these junctions has been shown to involve a protein-protein interaction between Nvj1p 13 on the nuclear envelope and Vac8p on the vacuolar membrane. 12 In addition to their well-established functions in oxidative phosphorylation and numerous metabolic pathways, the role played by mitochondrial homeostasis in cellular ageing and programmed cell death, has, in recent years, provided new investigative pathways to major physiological and pathological issues. 14 Indeed, mitochondria are both a major source and the main target of reactive oxygen species that are the principal cause of the alteration of biological structures.…”
Section: Introductionmentioning
confidence: 99%
“…Diverging descriptions of microautophagy have been documented in S. cerevisiae, concerning its dependence on the Apg/Aut pathway 12 and TOR. 13 In higher eukaryotes, complete autophagy is generally acknowledged as a PCD. Recent studies report that type II PCD (autophagy) could substitute for defective type I PCD (apoptosis), suggesting (i) that autophagy could be physiologically triggered by other means besides nutritional stress, and (ii) that autophagy could be an alternative to apoptosis.…”
Section: Introductionmentioning
confidence: 99%