PIE-1 is a bifunctional protein that regulates maternal and zygotic gene expression in the embryonic germ line of <i>Caenorhabditis elegans</i>

Tenenhaus, Christina; Subramaniam, Kuppuswamy; Dunn, Melanie A.; Seydoux, Géraldine

doi:10.1101/gad.876201

Cited by 188 publications

(164 citation statements)

References 66 publications

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“…For germline development, pie-1 and nos-2 control PGC fate and influence their incorporation into the gonadal primordium (Seydoux et al, 1996;Subramaniam and Seydoux, 1999;Tenenhaus et al, 2001). In this paper, we report that the C. elegans Hand bHLH transcription factor hnd-1 is important for early gonadogenesis as well as for embryogenesis.…”

Section: Introductionmentioning

confidence: 89%

TheC. elegansHand gene controls embryogenesis and early gonadogenesis

2003

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The C. elegans genome encodes a single Hand bHLH transcription factor. Either hnd-1(RNAi) or a hnd-1 deletion causes partially penetrant defects in viability and gonadogenesis. Dead embryos and young larvae are often misshapen at the posterior end. Our primary focus has been the role of hnd-1 in gonadogenesis. Wild-type C. elegans has two somatic gonadal precursors and two primordial germ cells in stereotyped positions within its four-celled gonadal primordium. The hnd-1 gene affects the presence and position of both the somatic gonadal precursors and primordial germ cells within the primordium, but does not appear to have any role in later gonadogenesis. hnd-1 probably acts within the somatic gonadal precursors or their mesodermal predecessors; defects in primordial germ cells and germ line appear to be secondary. In hnd-1 mutants, somatic gonadal precursors are generated normally, but are not maintained properly and sometimes die. A similar role in controlling the maintenance of precursor fates has been described for other genes governing early organogenesis, including the zebrafish Hand gene hands off. We also report the discovery of two genes, ehn-1 and ehn-3, that have overlapping functions with hnd-1 in embryogenesis and gonadogenesis.

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Section: Introductionmentioning

confidence: 89%

TheC. elegansHand gene controls embryogenesis and early gonadogenesis

2003

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“…OsDOS encodes a CCCH-type zinc finger protein without known signal peptide. Apparently, unlike the cytoplasm localization of Pie-1, Mex-1, and Pos-1, three Caenorhabditis elegans proteins with similar zinc fingers (Guedes and Priess, 1997; Tabara et al, 1999;Tenenhaus et al, 2001), OsDOS was found to be a nuclear-localized protein (Fig. 1B) similar to HUA1, a nuclear-localized CCCH-type zinc finger protein in Arabidopsis (Li et al, 2001).…”

Section: Osdos Encodes a Nuclear Protein Required For Delaying The Lementioning

confidence: 99%

A Novel Nuclear-Localized CCCH-Type Zinc Finger Protein, OsDOS, Is Involved in Delaying Leaf Senescence in Rice

et al. 2006

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Leaf senescence is a developmentally programmed degeneration process, which is fine tuned by a complex regulatory network for plant fitness. However, molecular regulation of leaf senescence is poorly understood, especially in rice (Oryza sativa), an important staple crop for more than half of the world population. Here, we report a novel nuclear-localized CCCH-type zinc finger protein, Oryza sativa delay of the onset of senescence (OsDOS), involved in delaying leaf senescence in rice. The expression of OsDOS was down-regulated during natural leaf senescence, panicle development, and pollination, although its transcripts were accumulated in various organs. RNAi knockdown of OsDOS caused an accelerated age-dependent leaf senescence, whereas its overexpression produced a marked delay of leaf senescence, suggesting that it acts as a negative regulator for leaf senescence. A genome-wide expression analysis further confirmed its negative regulation for leaf senescence and revealed that, in particular, the jasmonate (JA) pathway was found to be hyperactive in the OsDOS RNAi transgenic lines but impaired in the OsDOS overexpressing transgenic lines, indicating that this pathway is likely involved in the OsDOS-mediated delaying of leaf senescence. Furthermore, methyl JA treatments of both seeds and detached leaves from the RNAi and the overexpressing transgenic lines showed hyperand hyporesponses, respectively, consistent with the negative regulation of the JA pathway by OsDOS. Together, these results indicate that OsDOS is a novel nuclear protein that delays leaf senescence likely, at least in part, by integrating developmental cues to the JA pathway.

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“…We then investigated whether polarity cues regulate PLK-1 asymmetry separately from asymmetric spindle positioning and cell fate determination, both of which are also controlled by A-P polarity (reviewed by Gönczy and Rose, 2005). To this end, we examined goa-1/gpa-16(RNAi) embryos, in which the first division is equal as a result of symmetric spindle positioning (Colombo et al, 2003;Gotta and Ahringer, 2001), as well as pie-1(zu154) mutant embryos, in which the fate of the P 1 blastomere is altered (Tenenhaus et al, 1998;Tenenhaus et al, 2001). We found that PLK-1 distribution is asymmetric in such embryos (Fig.…”

Section: Research Articlementioning

confidence: 99%

PLK-1 asymmetry contributes to asynchronous cell division ofC. elegansembryos

Budirahardja

Gönczy

2008

Development

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Acquisition of lineage-specific cell cycle duration is an important feature of metazoan development. In Caenorhabditis elegans, differences in cell cycle duration are already apparent in two-cell stage embryos, when the larger anterior blastomere AB divides before the smaller posterior blastomere P 1 . This time difference is under the control of anterior-posterior (A-P) polarity cues set by the PAR proteins. The mechanisms by which these cues regulate the cell cycle machinery differentially in AB and P 1 are incompletely understood. Previous work established that retardation of P 1 cell division is due in part to preferential activation of an ATL-1/CHK-1 dependent checkpoint in P 1 , but how the remaining time difference is controlled is not known. Here, we establish that differential timing relies also on a mechanism that promotes mitosis onset preferentially in AB. The polo-like kinase PLK-1, a positive regulator of mitotic entry, is distributed in an asymmetric manner in two-cell stage embryos, with more protein present in AB than in P 1 . We find that PLK-1 asymmetry is regulated by A-P polarity cues through preferential protein retention in the embryo anterior. Importantly, mild inactivation of plk-1 by RNAi delays entry into mitosis in P 1 , but not in AB, in a manner that is independent of ATL-1/CHK-1. Together, our findings support a model in which differential timing of mitotic entry in C. elegans embryos relies on two complementary mechanisms: ATL-1/CHK-1-dependent preferential retardation in P 1 and PLK-1-dependent preferential promotion in AB, which together couple polarity cues and cell cycle progression during early development.

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PIE-1 is a bifunctional protein that regulates maternal and zygotic gene expression in the embryonic germ line of Caenorhabditis elegans

Cited by 188 publications

References 66 publications

TheC. elegansHand gene controls embryogenesis and early gonadogenesis

TheC. elegansHand gene controls embryogenesis and early gonadogenesis

A Novel Nuclear-Localized CCCH-Type Zinc Finger Protein, OsDOS, Is Involved in Delaying Leaf Senescence in Rice

PLK-1 asymmetry contributes to asynchronous cell division ofC. elegansembryos

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