Picture perfect: Imaging mitochondrial membrane potential changes in retina slices with minimal stray fluorescence

Haider, Syeda Zehra; Mohanraj, Nivedha; Markandeya, Yogananda S.; Joshi, Preeti G.; Mehta, Bhupesh

doi:10.1016/j.exer.2020.108318

Cited by 6 publications

(3 citation statements)

References 30 publications

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“…In the present study, LPS‐treated mouse thymus tissue had increased ROS level and MDA level, and decreased mitochondrial membrane potential and SOD levels. However, EGCG administration normalized alterations in mitochondrial membrane potential, a key indicator for cell health and viability, 74,75 ROS level and oxidative and anti‐oxidative indexes MDA and SOD, 76,77 induced by LPS injection. It was suggested that EGCG could alleviate LPS‐induced oxidative stress in mouse thymus and protect mitochondrial function.…”

Section: Discussionmentioning

confidence: 95%

Epigallocatechin‐3‐gallate ameliorates lipopolysaccharide‐induced acute thymus involution in mice via AMPK/Sirt1 pathway

Su,

Yang,

Guo

et al. 2024

Microbiology and Immunology

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The thymus, a site to culture the naïve T lymphocytes, is susceptible to atrophy or involution due to aging, inflammation, and oxidation. Epigallocatechin‐3‐gallate (EGCG) has been proven to possess anti‐inflammatory, antioxidant, and antitumor activity. Here, we investigate the effects of EGCG on thymic involution induced by lipopolysaccharide (LPS), an endotoxin derived from Gram‐negative bacteria. The methodology included an in vivo experiment on female Kunming mice exposed to LPS and EGCG. Morphological assessment of thymic involution, immunohistochemical detection, and thymocyte subsets analysis by flow cytometry were further carried out to evaluate the potential role of EGCG on the thymus. As a result, we found that EGCG alleviated LPS‐induced thymic atrophy, increased mitochondrial membrane potential and superoxide dismutase levels, and decreased malondialdehyde and reactive oxygen species levels. In addition, EGCG pre‐supplement restored the ratio of thymocyte subsets, the expression of autoimmune regulator, sex‐determining region Y‐box 2, and Nanog homebox, and reduced the number of senescent cells and collagen fiber deposition. Western blotting results indicated that EGCG treatment elevated LPS‐induced decrease in pAMPK, Sirt1 protein expression. Collectively, EGCG relieved thymus architecture and function damaged by LPS via regulation of AMPK/Sirt1 signaling pathway. Our findings may provide a new strategy on protection of thymus from involution caused by LPS by using EGCG. And EGCG might be considered as a potential agent for the prevention and treatment of thymic involution.

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Section: Discussionmentioning

confidence: 95%

Epigallocatechin‐3‐gallate ameliorates lipopolysaccharide‐induced acute thymus involution in mice via AMPK/Sirt1 pathway

Su,

Yang,

Guo

et al. 2024

Microbiology and Immunology

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“…ATP is generated in the mitochondria through the electron transport chain by creating an electrochemical gradient through a series of redox reactions [52,65]. This electrochemical gradient generates MMP, which is a direct measure of ATP production ability and a key metric for evaluating mitochondrial function and overall cellular health [65,66]. A decrease in MMP is one of the main features of mitochondrial dysfunction, and this decrease has been linked to apoptosis [52,66,67].…”

Section: Evaluating the Mitoprotective Effects Of Fractions By Mitoch...mentioning

confidence: 99%

A High-Throughput Screening of a Natural Products Library for Mitochondria Modulators

Makinde,

Ma,

Mellick

et al. 2024

Biomolecules

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Mitochondria, the energy hubs of the cell, are progressively becoming attractive targets in the search for potent therapeutics against neurodegenerative diseases. The pivotal role of mitochondrial dysfunction in the pathogenesis of various diseases, including Parkinson’s disease (PD), underscores the urgency of discovering novel therapeutic strategies. Given the limitations associated with available treatments for mitochondrial dysfunction-associated diseases, the search for new potent alternatives has become imperative. In this report, we embarked on an extensive screening of 4224 fractions from 384 Australian marine organisms and plant samples to identify natural products with protective effects on mitochondria. Our initial screening using PD patient-sourced olfactory neurosphere-derived (hONS) cells with rotenone as a mitochondria stressor resulted in 108 promising fractions from 11 different biota. To further assess the potency and efficacy of these hits, the 11 biotas were subjected to a subsequent round of screening on human neuroblastoma (SH-SY5Y) cells, using 6-hydroxydopamine to induce mitochondrial stress, complemented by a mitochondrial membrane potential assay. This rigorous process yielded 35 active fractions from eight biotas. Advanced analysis using an orbit trap mass spectrophotometer facilitated the identification of the molecular constituents of the most active fraction from each of the eight biotas. This meticulous approach led to the discovery of 57 unique compounds, among which 12 were previously recognized for their mitoprotective effects. Our findings highlight the vast potential of natural products derived from Australian marine organisms and plants in the quest for innovative treatments targeting mitochondrial dysfunction in neurodegenerative diseases.

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“…It is a sensitive indicator for evaluating mitochondrial function (90)(91)(92)(93). It is closely associated with cellular homeostasis and is most commonly used to determine the metabolic state of mitochondria (93)(94)(95)(96)(97)(98).…”

Section: Determination Of Mitochondrial Membrane Potentialmentioning

confidence: 99%

Common methods in mitochondrial research (Review)

Yin

Shen

2022

Int J Mol Med

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Mitochondrial abnormalities are primarily seen in morphology, structure and function. They can cause damage to organs, including the heart, brain and muscle, by various mechanisms, such as oxidative stress, abnormal energy metabolism, or genetic mutations. Identifying and detecting pathophysiological alterations in mitochondria is the principal means of studying mitochondrial abnormalities. The present study reviewed methods in mitochondrial research and focused on three aspects: Mitochondrial extraction and purification, morphology and structure and function. In addition to classical methods, such as electron microscopy and mitochondrial membrane potential monitoring, newly developed methods, such as mitochondrial ultrastructural determination, mtdNA mutation assays, metabolomics and analyses of regulatory mechanisms, have also been utilized in recent years. These approaches enable the accurate detection of mitochondrial abnormalities and provide guidance for the diagnosis and treatment of related diseases. Contents1. Introduction 2. Extraction and purification of mitochondria 3. determination of mitochondrial morphology and structure 4. determination of mitochondrial function 5.

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Picture perfect: Imaging mitochondrial membrane potential changes in retina slices with minimal stray fluorescence

Cited by 6 publications

References 30 publications

Epigallocatechin‐3‐gallate ameliorates lipopolysaccharide‐induced acute thymus involution in mice via AMPK/Sirt1 pathway

Epigallocatechin‐3‐gallate ameliorates lipopolysaccharide‐induced acute thymus involution in mice via AMPK/Sirt1 pathway

A High-Throughput Screening of a Natural Products Library for Mitochondria Modulators

Common methods in mitochondrial research (Review)

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