Picosecond laser photolysis studies on the photorepair of pyrimidine dimers by DNA photolyase. 1. Laser photolysis of photolyase-2-deoxyuridine dinucleotide photodimer complex

Mechanisms of DNA Repair by Photolyase and Excision Nuclease (Nobel Lecture)

“…"After months of work, Isuccessfully cloned the gene. [13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28] Moreover,wefound that the enzyme exhibits colors ranging from purple to orange depending on the redox state of the flavin cofactor [29] (Figure 3). In subsequent years at the University of North Carolina, my colleagues and I used the cloned gene to purify the enzyme in gram quantities ( Figure 2, middle), [10][11][12] and while purifying it we found that it has ab right blue color (Figure 2, right).…”

DNA Repair Mechanism by Photolyase: Electron Transfer Path from the Photolyase Catalytic Cofactor FADH−to DNA Thymine Dimer

“…This latter result strongly suggests that the enzyme predominantly recognizes the dimer rather than any distortion of the helix (Sancar et al, 1985). The rate and e$ciency of single electron transfer from *FADH\ to Pyr12Pyr have been investigated by time-resolved #uorescence, absorbance (Langenbacher et al, 1997;Kim et al, 1994Kim et al, , 1992aOkamura et al, 1991), and EPR spectroscopies (Kim et al, 1992c).…”

Photo-CIDNP study of the splitting of the dinucleotidecis, syn-thymine dimer with reduced flavin as a sensitizer: Evidence for a thymine radical anion intermediate

“…Photolyases contain two chromophores, one of which is reduced flavin adenine dinucleotide, FADH 2. It has been demonstrated that this is the chromophore involved in the electron transfer reaction with the thymine dimer [4,5]. Two mechanisms have been suggested for the enzymatic reaction: either electron transfer of the excited chromophore FADH2* to the thymine dimer or electron transfer from dimer to chromophore.…”