Picomole determination of 2,4-dimethoxyanilides of prostaglandins by high-performance liquid chromatography with electrochemical detection

Knospe, Johanna; Steinhilber, Dieter; Herrmann, Thomas; Roth, H. J.

doi:10.1016/s0021-9673(00)94498-4

Cited by 16 publications

(2 citation statements)

References 16 publications

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“…After 1 h at 37 °C, 1.6 ml of MeOH was added, and arachidonic acid was extracted by RP-18 solid phase extraction. Following derivatization with p -anisidinium chloride, the resulting derivate was analyzed by RP-HPLC at 249 nm (25). …”

Section: Methodsmentioning

confidence: 99%

12/15-Lipoxygenase Contributes to Platelet-derived Growth Factor-induced Activation of Signal Transducer and Activator of Transcription 3

Blažević

Schwaiberger

Schreiner

et al. 2013

Journal of Biological Chemistry

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Background: The small molecule indirubin-3′-monoxime (I3MO) inhibits activation of STAT3 in vascular smooth muscle cells, with an unresolved mechanism.Results: Activation of 12/15-lipoxygenase (LO) is crucial for PDGF-induced Src and STAT3 activation and is impaired by I3MO.Conclusion: I3MO interferes with PDGFR-Src-STAT3 signaling via impaired 12/15-LO activation.Significance: 12/15-LO is an important signaling hub within the PDGF-STAT3 pathway.

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Section: Methodsmentioning

confidence: 99%

12/15-Lipoxygenase Contributes to Platelet-derived Growth Factor-induced Activation of Signal Transducer and Activator of Transcription 3

Blažević

Schwaiberger

Schreiner

et al. 2013

Journal of Biological Chemistry

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“…After 1 h at 37°C, 1.6 mL MeOH was added, and AA was extracted by RP‐18 solid phase extraction. Following derivatization with p ‐anisidium chloride, the resulting mixture was analysed by RP‐HPLC at 249 nm, as described by Knospe et al . (1988).…”

Section: Methodsmentioning

confidence: 99%

The 5‐lipoxygenase inhibitor, zileuton, suppresses prostaglandin biosynthesis by inhibition of arachidonic acid release in macrophages

Rossi

Pergola

Koeberle

et al. 2010

British J Pharmacology

117

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BACKGROUND AND PURPOSEZileuton is the only 5-lipoxygenase (5-LOX) inhibitor marketed as a treatment for asthma, and is often utilized as a selective tool to evaluate the role of 5-LOX and leukotrienes. The aim of this study was to investigate the effect of zileuton on prostaglandin (PG) production in vitro and in vivo. EXPERIMENTAL APPROACHPeritoneal macrophages activated with lipopolysaccharide (LPS)/interferon g (LPS/IFNg), J774 macrophages and human whole blood stimulated with LPS were used as in vitro models and rat carrageenan-induced pleurisy as an in vivo model. KEY RESULTSZileuton suppressed PG biosynthesis by interference with arachidonic acid (AA) release in macrophages. We found that zileuton significantly reduced PGE2 and 6-keto prostaglandin F1a (PGF1a) levels in activated mouse peritoneal macrophages and in J774 macrophages. This effect was not related to 5-LOX inhibition, because it was also observed in macrophages from 5-LOX knockout mice. Notably, zileuton inhibited PGE2 production in LPS-stimulated human whole blood and suppressed PGE2 and 6-keto PGF1a pleural levels in rat carrageenan-induced pleurisy. Interestingly, zileuton failed to inhibit the activity of microsomal PGE2 synthase1 and of cyclooxygenase (COX)-2 and did not affect COX-2 expression. However, zileuton significantly decreased AA release in macrophages accompanied by inhibition of phospholipase A2 translocation to cellular membranes. CONCLUSIONS AND IMPLICATIONZileuton inhibited PG production by interfering at the level of AA release. Its mechanism of action, as well as its use as a pharmacological tool, in experimental models of inflammation should be reassessed. Abbreviations

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Dietary linolenic acid-mediated increase in vascular prostacyclin formation

et al. 1996

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To define vascular effects of an enhanced dietary alpha-linolenic acid intake, 28 spontaneously hypertensive rats were fed a 3% sunflowerseed oil (44% linoleic acid) diet; in 3 groups (7 rats each), the diet was supplemented with 1, 2.5 or 5% linseed oil containing 62% alpha-linolenic acid. alpha-Linolenic acid was incorporated up to 12% in the aorta of the 5% linseed oil group. The eicosapentaenoic acid content was not significantly increased. The content of arachidonic acid and docosatetraenoic acid was moderately reduced in rats fed 5% linseed oil. The generation of 6-keto-PGF1 alpha (degradation product of prostacyclin) assessed by HPLC/electrochemical detection was, however, markedly increased (p < 0.05) in rats fed 2.5 and 5% linseed oil. The minor prostanoids TXB2, PGE2 and PGF2 alpha were not significantly altered. The high systolic and diastolic blood pressure of SHR monitored by radio telemetry was more effectively reduced (p < 0.05) in the light, i.e. sleep, cycle. An increased prostacyclin formation and lowered vascular arachidonic acid content associated with enhanced dietary alpha-linolenic acid intake would thus be expected to prove beneficial in the prevention of vascular disorders.

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Picomole determination of 2,4-dimethoxyanilides of prostaglandins by high-performance liquid chromatography with electrochemical detection

Cited by 16 publications

References 16 publications

12/15-Lipoxygenase Contributes to Platelet-derived Growth Factor-induced Activation of Signal Transducer and Activator of Transcription 3

12/15-Lipoxygenase Contributes to Platelet-derived Growth Factor-induced Activation of Signal Transducer and Activator of Transcription 3

The 5‐lipoxygenase inhibitor, zileuton, suppresses prostaglandin biosynthesis by inhibition of arachidonic acid release in macrophages

Dietary linolenic acid-mediated increase in vascular prostacyclin formation

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