Pichia ohmeri fungemia associated with phlebitis: successful treatment with amphotericin B

Shin, Dong-Hyeon; Park, Jeong Ho; Kim, Sei Jong; Shin, Jong Hee; Suh, Soon Pal; Ryang, Dong‐Wook

doi:10.1007/s10156-002-0208-z

Cited by 29 publications

(17 citation statements)

References 9 publications

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Section: Discussionsupporting

confidence: 85%

“…In this study, similar to previous reports, the two isolates of K. ohmeri were susceptible to amphotericin B, but the patients were not treated probably for the known side effect of the drug. 4,5,[10][11][12] To date, there is no evidence that any antifungal agent is superior to the others in the treatment of K. ohmeri infections and few data exist regarding the susceptibility of K. ohmeri to the azoles and echinocandins. 4,5,13,14 In conclusion, the current report confirmed that K. ohmeri is an emerging opportunistic pathogen in immunocompromised patients and that molecular analysis is useful for species identification, although further studies are needed to establish the optimal antifungal regimens.…”

Section: Discussionmentioning

confidence: 99%

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Kodamaea ohmeri isolate from two immunocompromised patients: first report in Italy

Santino

Bono²,

Borruso

et al. 2012

Mycoses

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Kodamaea ohmeri is an unusual yeast-form fungus that has recently been identified as an important aetiological agent of fungaemia, endocarditis, cellulitis, funguria and peritonitis in immunocompromised patients. We present two new isolated of K. ohmeri. The microorganisms were identified by CHROMagar Candida medium, VitekII system and API ID32C. Biochemical identification of the two yeast isolates was confirmed by sequence analysis of the 26S ribosomal DNA. Antifungal susceptibility testing done by Sensititre YeastOne showed that the isolates were susceptible to amphotericin B, voriconazole and itraconazole. This work is the first report of isolation of K. ohmeri in immunocompromised patients in Italy.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Kodamaea ohmeri isolate from two immunocompromised patients: first report in Italy

Santino

Bono²,

Borruso

et al. 2012

Mycoses

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“…Nine cases of K. ohmeri fungemia have been previously reported, including one case of K. ohmeri fungemia-associated phlebitis (in one of the patients included in our study) (12). Here, we report an additional five cases of K. ohmeri-related fungemia identified at our hospital over a 5-year period ( Table 3).…”

Section: Fig 1 Ek Of Yeast Isolates Identified As K Ohmeri By Usinmentioning

confidence: 57%

“…membranaefaciens, is an environmental strain commonly used in the food industry for the fermentation of pickles, rinds, and fruit; however, it is also an emerging fungal pathogen, particularly in immunocompromised patients (3,7). To date, 12 cases of K. ohmeri infection have been reported, including nine cases presenting with fungemia plus two cases that occurred as a nosocomial cluster (3,7,12). Although molecular biological techniques that allow more precise identification and genotyping of yeast species have recently been developed, little research has been conducted on identifying and genotyping the clinical isolates of K. ohmeri.…”

mentioning

confidence: 99%

Kodamaea ohmeri Isolates from Patients in a University Hospital: Identification, Antifungal Susceptibility, and Pulsed-Field Gel Electrophoresis Analysis

Lee¹,

Shin²,

Kim

et al. 2007

J Clin Microbiol

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Data on clinical isolates of Kodamaea (Pichia) ohmeri, an emerging fungal pathogen, are scarce. Over the past 5 years, we identified yeast isolates from nine patients with fungemia as K. ohmeri by using the API 20C system. Here, we reanalyzed these isolates first by sequencing the internal transcribed spacer 2 (ITS2) regions and then by growing the isolates on CHROMagar Candida medium and subjecting them to pulsed-field gel electrophoresis (PFGE). Based on their ITS2 sequences, six of the nine isolates were confirmed as K. ohmeri, while the others were identified as Candida haemulonii (n ‫؍‬ 2) and Candida parapsilosis (n ‫؍‬ 1). PFGE karyotyping of the K. ohmeri isolates revealed similar major bands, and their colonies showed a characteristic color change from pink to blue when grown on CHROMagar Candida medium for more than 48 h. For K. ohmeri, the ranges of MICs of fluconazole, voriconazole, caspofungin, and micafungin were 2 to 32 g/ml, 0.03 to 0.5 g/ml, 0.125 to 0.25 g/ml, and 0.03 to 0.06 g/ml, respectively. Restriction endonuclease analysis of genomic NotI-digested DNA (REAG-N) from isolates from different patients produced unique patterns, suggesting that the fungemia had occurred sporadically. This study determined that ITS2 sequence data, PFGE karyotypes, and CHROMagar Candida chromogenic culture medium are reliable diagnostic tools for identifying K. ohmeri while REAG-N is useful for genotyping the clinical isolates of K. ohmeri.

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“…In an Australian survey of candidemic episodes, only one isolate of C. lambica was identified from 1,095 cases over a 4-year period (9). Similarly, there have been few reports documenting fungemia due to K. ohmeri (4,19,20,33,41,45) and C. nivariensis (1,5,16). Targets for these organisms can be added to the MT-PCR in the future if there are increasing reports of their causing candidemia.…”

Section: Discussionmentioning

confidence: 99%

Multiplex Tandem PCR: a Novel Platform for Rapid Detection and Identification of Fungal Pathogens from Blood Culture Specimens

et al. 2008

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We describe the first development and evaluation of a rapid multiplex tandem PCR (MT-PCR) assay for the detection and identification of fungi directly from blood culture specimens that have been flagged as positive. The assay uses a short-cycle multiplex amplification, followed by 12 simultaneous PCRs which target the fungal internal transcribed spacer 1 (ITS1) and ITS2 region, elongation factor 1-␣ (EF1-␣), and ␤-tubulin genes to identify 11 fungal pathogens: Candida albicans, Candida dubliniensis, Candida glabrata, Candida guilliermondii, Candida krusei, Candida parapsilosis complex, Candida tropicalis, Cryptococcus neoformans complex, Fusarium solani, Fusarium species, and Scedosporium prolificans. The presence or absence of a fungal target was confirmed by melting curve analysis. Identification by MT-PCR correlated with culture-based identification for 44 (100%) patients. No crossreactivity was detected in 200 blood culture specimens that contained bacteria or in 30 blood cultures without microorganisms. Fungi were correctly identified in five specimens with bacterial coinfection and in blood culture samples that were seeded with a mixture of yeast cells. The MT-PCR assay was able to provide rapid (<2 h), sensitive, and specific simultaneous detection and identification of fungal pathogens directly from blood culture specimens.

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Pichia ohmeri fungemia associated with phlebitis: successful treatment with amphotericin B

Cited by 29 publications

References 9 publications

Kodamaea ohmeri isolate from two immunocompromised patients: first report in Italy

Kodamaea ohmeri isolate from two immunocompromised patients: first report in Italy

Kodamaea ohmeri Isolates from Patients in a University Hospital: Identification, Antifungal Susceptibility, and Pulsed-Field Gel Electrophoresis Analysis

Multiplex Tandem PCR: a Novel Platform for Rapid Detection and Identification of Fungal Pathogens from Blood Culture Specimens

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