Piceatannol Affects Gastric Ulcers Induced by Indomethacin: Association of Antioxidant, Anti-Inflammatory, and Angiogenesis Mechanisms in Rats

Shaik, Rasheed A.; Eid, Basma G.

doi:10.3390/life12030356

Cited by 17 publications

(14 citation statements)

References 52 publications

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“…The NF‐κB expression was paralleled by a strong COX‐2 expression in the same animal group. In accordance, Mostafa et al 3 and Shaik and Eid 38 recorded an overexpression of NF‐κB and COX‐2 in a parallel model of gastric ulceration. Soyasaponin I treatment showed a marked reduction in the mean area percent of NF‐κB and COX‐2 immunoexpression, and a similar effect was observed at the gene level.…”

Section: Discussionsupporting

confidence: 71%

Targeting NF‐κB/COX‐2 signaling by soyasaponin I alleviates diclofenac‐induced gastric ulceration in male albino rats

Morsi,

Shawky,

Shawky

et al. 2024

Cell Biochemistry & Function

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Gastric ulceration is a prevalent worldwide clinical presentation due to altered gastric defense mechanisms. Nonsteroidal anti‐inflammatory drugs are one of the common causes of gastric ulcers mediated by the release of inflammatory mediators. The study aimed to investigate the potential protective effect of soyasaponin I (soya) against diclofenac (DIC)‐induced gastric ulcer in rats and to highlight the underlying mechanisms. The experiment was conducted on 40 male Wistar albino rats, equally distributed into five groups: control, DIC‐induced ulcer (9 mg/kg/d, orally, twice daily for 3 days), ulcer/soya‐, ulcer/ranitidine‐, and ulcer/soya/selective nuclear factor kappa B inhibitor (JSH‐23)‐treated groups. The doses of soya, ranitidine, and JSH were 20, 25, and 5 mg/kg/d, respectively, given orally. Gastric specimens were prepared for gene and histological study and for biochemical analysis of gastric prostaglandin E2 (PGE2), oxidative markers, and inflammatory cytokines. The gastric samples were formalin‐fixed, paraffin‐embedded, and subjected to hematoxylin and eosin (H&E), PAS staining, and immunohistochemical assay for identification of nuclear factor kappa B (NF‐κB), cyclooxygenase‐2 (COX‐2), and proliferation marker (Ki67) expressions. The findings revealed decreased gastric PGE2 and altered inflammatory and oxidative markers in the ulcer model group. The H&E staining showed mucosal injury characterized by mucosal surface defects and inflammatory cell infiltrations. The polymerase chain reaction (PCR) and immunohistochemistry demonstrated an upregulation of NF‐κB and COX‐2 expression at gene/protein levels; meanwhile, Ki67 downregulation. The soya‐treated group showed maintained biochemical, histological, and PCR findings comparable to the ranitidine‐treated group. The JSH‐23‐treated group still showed partial gastric protection with biochemical and immunohistochemical changes. Soyasaponin I ameliorated DIC‐induced gastric ulcers by targeting the COX‐2 activity through modulation of NF‐κB signaling.

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Section: Discussionsupporting

confidence: 71%

Targeting NF‐κB/COX‐2 signaling by soyasaponin I alleviates diclofenac‐induced gastric ulceration in male albino rats

Morsi,

Shawky,

Shawky

et al. 2024

Cell Biochemistry & Function

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“…The malignancies and the inflammatory reactions are the general outcomes of the toxicity of insecticides and pesticides. Non-steroidal anti-inflammatory drugs (NSAIDs) are the most extensively used analgesic and anti-inflammatory drugs but these are also the major causative agent that not only induces gastric damage but via various mechanisms, also delay the process of healing 3 . The topic of detoxification of these chemicals remained the topic of interest for the avoidance of such health damage.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the anti-inflammatory, analgesic, anti-pyretic and anti-ulcerogenic potentials of synthetic indole derivatives

Siddique

Ahmad

Nawaz

et al. 2023

Sci Rep

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A large number of new synthetic compounds are synthesized in the field of heterocyclic chemistry having a variety of biological potentials. In the present study, some synthetic indole derivatives are used to check anti-inflammatory, analgesic, antipyretic and gastroprotective activity in albino mice. Albino mice of either sex of reproductive age were used for each study (n = 5). In anti-inflammatory activity, the negative control (NC) and positive control group animals were treated with normal saline and 10 mg/kg of indomethacin respectively. The treated groups received the twenty four different synthetic chemicals, after 30 min of sub cutaneous injection of carrageenan. In analgesic activity, hot-plate method is used and for each group the latency period was recorded at zero moment of the provision of required dose and after 30, 60, 90, 120 and 180 min. In anti-pyretic activity, Pyrexia was induced by using Brewer's yeast method. Before any treatment and then after duration of 18 h, the rectal temperatures were recorded. Among all the chemicals, only those chemicals which show any potential related to above mentioned activities were selected for gastroprotective activity. The gastroprotective activity was performed to check the gastric ulcers by using 300 mg/kg of single oral dose of indomethacin to animals of all groups except NC group. This study helped to screen out the most potent indole derivatives 3a-II and 4a-II from the 24 synthetic indole derivatives which demonstrated the best biological potential (anti-inflammatory, analgesic, antipyretic, and gastroprotection) as compared to the remaining ones. The micrometric and biochemical results also support the histological findings. Out of the twenty-four novel indole amines tested, 3a-II and 4a-II have shown the effective pharmacological capacity and additionally have not shown any overt and systemic toxicity. Thus these two indole amines need further in-depth pharmacokinetic and pharmacodynamics studies before they are recommended for any pre-clinical trial.

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“…Similarly, this study revealed the effects of Cur on the oxidative stress, inflammation, and apoptosis of gastric epithelial cells. Cur has also been reported to ameliorate bone loss by affecting mesenchymal stem cell fate in aging mice [17]. Furthermore, Cur promotes osteogenic differentiation, preventing ovariectomized-induced osteoporosis.…”

Section: Discussionmentioning

confidence: 99%

Curculigoside attenuates Helicobacter pylori-induced inflammation and apoptosis of gastric mucosal epithelial cells via NF-κB pathway

Li¹,

Su²

2023

Trop. J. Pharm Res

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Purpose: To investigate the possible effects and mechanisms of action of curculigoside (Cur) on gastric ulcers. Methods: Human gastric mucosal epithelial GES-1 cells were infected with Helicobacter pylori and then treated with Cur. Cell counting kit 8 (CCK-8), flow cytometry, and immunofluorescence assays were used to investigate the effect of Cur on cell viability and apoptosis after exposure to H. pylori. Inflammation status and reactive oxygen species (ROS) were assessed using enzyme-linked immunoassay (ELISA) and dichlorodihydrofluorescein (DCF) staining, respectively, while immunoblot and immunofluorescence assays were performed to determine the effect of Cur on the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway. Results: Cur significantly increased H. pylori-induced cell viability and inhibited H. pylori-induced inflammatory cytokine production (p < 0.01). Furthermore, Cur significantly suppressed H. pylori-induced ROS production and apoptosis (p < 0.01). Through the NF-κB pathway, Cur attenuated H. pylori-induced inflammation and apoptosis of gastric mucosal epithelial cells. Conclusion: In H. pylori infection, Cur treatment increases cell viability, reduces inflammatory cytokine production, suppresses ROS production, and inhibits apoptosis via NF-κB pathway. Further investigation using whole animal experiments would be needed to establish the role of Cur in the management of H. pylori-induced gastric ulcers.

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Piceatannol Affects Gastric Ulcers Induced by Indomethacin: Association of Antioxidant, Anti-Inflammatory, and Angiogenesis Mechanisms in Rats

Cited by 17 publications

References 52 publications

Targeting NF‐κB/COX‐2 signaling by soyasaponin I alleviates diclofenac‐induced gastric ulceration in male albino rats

Targeting NF‐κB/COX‐2 signaling by soyasaponin I alleviates diclofenac‐induced gastric ulceration in male albino rats

Evaluation of the anti-inflammatory, analgesic, anti-pyretic and anti-ulcerogenic potentials of synthetic indole derivatives

Curculigoside attenuates Helicobacter pylori-induced inflammation and apoptosis of gastric mucosal epithelial cells via NF-κB pathway

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