Phytotoxicity of short-term exposure to excess zinc or copper in Scots pine seedlings in relation to growth, water status, nutrient balance, and antioxidative activity

Ivanov, Yu. V.; Иванова, А. И.; Карташов, А. В.; Кузнецов, В. В.

doi:10.1007/s11356-020-11723-x

Cited by 8 publications

(7 citation statements)

References 42 publications

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“…The minimum Mn contents noted earlier in the organs of 6-week-old Scots pine seedlings under the action of zinc [ 17 , 18 ] or copper [ 18 , 23 ] were several times higher than those in the needles and ten times higher than those in the roots of 6-week-old Mn-deficient plants. Consequently, disturbances in Mn uptake by pine seedlings observed under heavy metal stress are unlikely to cause growth retardation per se .…”

Section: Discussionmentioning

confidence: 99%

“…The dry weight was determined using an analytical balance (AB54-S, Mettler Toledo, Switzerland) with an accuracy of 0.1 mg after drying the samples to a constant weight at 70 °C. The water content of each organ is expressed as a percentage of its fresh weight [ 18 ].…”

Section: Methodsmentioning

confidence: 99%

“…Seed Mn content was determined in full seeds prepared as described above. The samples were then digested in solutions of concentrated HNO 3 and HClO 4 (2:1 ( v / v )), after which the nutrient contents (except P) were determined by atomic absorption spectrometry [ 17 , 18 ]. Phosphorous was determined spectrophotometrically using a molybdenum blue reaction [ 25 ] as described in Ivanov et al [ 15 ].…”

Section: Methodsmentioning

confidence: 99%

“…Nevertheless, even for one species (specifically Scots pine) Mn contents varied considerably within a given stand for 17 consecutive years (range factor of 5.0) [ 1 ]. Moreover, variation (up to 76.4%) in Mn content was noted for needles of Scots pine seedlings grown in hydroculture, while available Mn concentrations in the nutrient solutions were the same [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

“…Thus, the stability and productivity of pine stands may depend on Mn availability. Previously, we found that in response to different stressors, such as heavy metals [ 17 , 18 , 23 ], soil alkalization [ 24 ] or water shortages [ 15 ], the Mn content in the organs of Scots pine changed much more strongly than other nutrients. In most cases, these changes were associated with a drastic decrease in the Mn content in plant organs.…”

Section: Introductionmentioning

confidence: 99%

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Manganese Deficiency Suppresses Growth and Photosynthetic Processes but Causes an Increase in the Expression of Photosynthetic Genes in Scots Pine Seedlings

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Иванова

et al. 2022

Cells

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Manganese deficiency is a serious plant nutritional disorder, resulting in the loss of crop productivity in many parts of the world. Despite the progress made in the study of angiosperms, the demand for Mn in gymnosperms and the physiological responses to Mn deficiency remain unexplored. We studied the influence of Mn deficiency for 24 weeks on Pinus sylvestris L. seedling growth, ion homeostasis, pigment contents, lipid peroxidation, chlorophyll fluorescence indices and the transcript levels of photosynthetic genes and genes involved in chlorophyll biosynthesis. It was shown that Mn-deficient plants demonstrated suppressed growth when the Mn content in the needles decreased below 0.34 µmol/g DW. The contents of photosynthetic pigments decreased when the Mn content in the needles reached 0.10 µmol/g DW. Mn deficiency per se did not lead to a decrease in the nutrient content in the organs of seedlings. Photoinhibition of PSII was observed in Mn-deficient plants, although this was not accompanied by the development of oxidative stress. Mn-deficient plants had an increased transcript abundance of genes (psbO, psbP, psbQ, psbA and psbC), encoding proteins directly associated with the Mn cluster also as other proteins involved in photosynthesis, whose activities do not depend on Mn directly. Furthermore, the transcript levels of the genes encoding the large subunit of Rubisco, light-dependent NADPH-protochlorophyllide oxidoreductase and subunits of light-independent protochlorophyllide reductase were also increased in Mn-deficient plants.

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