Phytosynthesis of Silver Nanoparticles: Characterization, Biocompatibility Studies, and Anticancer Activity

Abstract

Silver nanoparticles (SNPs), owing to their wide range of biomedical applications, have recently attracted remarkable interest for use in cancer nanomedicine. The present research work investigated the anticancer activity of phytosynthesized SNPs against human cancer cell lines. Phytosynthesis of SNPs was achieved by using an aqueous extract of Salacia chinensis (SC) bark as a green source to reduce silver nitrate to silver nanoparticles. Characterization of synthesized nanoparticles demonstrated a UV–visible peak at 443 nm, ζ-potential (zetasizer) of −25.6 ± 0.34 and particle size (transmission electron microscopy analysis) in the range of 40–80 nm, which validates formation of stable silver nanoparticles. The absence of cytotoxicity against normal human fibroblasts and blood erythrocytes confirms the biocompatible nature of green synthesized SNPs. In vitro anticancer assay demonstrated IC50 values of 6.31, 4.002, 5.228, 8.452, 14.37, 7.46, and 6.55 μg/mL against liver (Hep G2), lungs (L-132), pancreas (MIA-Pa-Ca-2), breast (MDA-MB-231), oral (KB cells), prostate (PC-3), and cervical (HeLa) cancer cell lines respectively, which confirms its potent anticancer action. The results of the present study give an experimental proof that the SC mediated green synthesized SNPs could serve as a promising anticancer agent to overcome limitations of existing conventional cancer chemotherapeutics.

Antiproliferative Activity of Elephantopus Scaber Mediated Silver Nanoparticles Against McF-7, a-549, SCC-40, and Colo-205 Human Cancer Cell Lines

“…The in vitro anticancer assay demonstrated an IC 50 =14.37 μg/ml against oral (KB cells) cancer cell lines which confirms its potent anticancer action [15]. Furthermore, the AgNPs synthesized using leaf extract of Piper betle revealed to have strong activity against oral (KB) carcinoma cells by MTT assay [16].…”

Silver Metallic Cyclodextrin‐Core Star mPEG

“…III was used as a reactive star polymer template and four recipes were investigated for preparation of SM‐CD‐SPEG (Scheme g), which were involved in changes in III and sodium hydroxide concentrations ( Table 1 ). The formation of nano‐sized silver particles in the experiments were monitored by UV–vis spectra (Figure S6, Supporting Information), because they can exhibit a characteristic absorbance at ≈410 nm shows changes of absorbance of the four reaction systems at 410 nm with time.…”

“…The formation of nano-sized silver particles in the experiments were monitored by UV-vis spectra ( Figure S6, Supporting Information), because they can exhibit a characteristic absorbance at ≈410 nm. [32][33][34] Figure 1 shows changes of absorbance of the four reaction systems at 410 nm with time. As seen from Figure 1, relatively speaking, E50A exhibited the most evident change of absorbance with time.…”

Sweet Corn (Zea mays L. var. rugosa) Derived Fluorescent Carbon Quantum Dots for Selective Detection of Hydrogen Sulfide and Bioimaging Applications

“…The hemolytic assay is a facile method, which is commonly used in the clinical and research laboratories to analyze the hemocompatiability of various biomedical materials . The nanoparticles (NPs) exposed into the blood system can cause RBC membrane damage and cell death, which may also lead to hemolysis .…”