Phytosynthesis of poly (ethylene glycol) methacrylate-hybridized gold nanoparticles from C. tuberculata: their structural characterization and potential for in vitro growth in banana

Anwar, Natasha; Wahid, Jan; Uddin, Jalal; Khan, Abbas; Shah, Mohib; Shah, Syed Adil; Subhan, Fazle; Khan, Mubarak Ali; Ali, Kazim; Rauf, Mamoona; Arif, Muhammad

doi:10.1007/s11627-020-10150-4

Cited by 17 publications

(11 citation statements)

References 54 publications

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“…The DPPH radical scavenging activity is widely used to determine the antioxidant activity of the plant. Our results are in accordance with [ 33 ], where there was increased phenol, flavonoid, and DPPH radical activity under the Ca-AuNPs and PEGMA-AuNPs in the in vitro grown banana. Other researchers under in vitro conditions also observed similar results [ 19 , 50 ].…”

Section: Discussionsupporting

confidence: 92%

“…In the present study, the application of citrate-AuNPs and CTAB-AuNPs significantly increased the shoot multiplication, root proliferation, total plant biomass, photosynthetic pigments, enzymatic, and non-enzymatic antioxidant levels and endogenous hormone levels in a dose dependent manner under in vitro conditions in N. jatamansi . It was observed earlier that green-synthesized biogenic Ca-AuNPs and PEGMA-AuNPs enhanced the fresh weight, dry weight, and total biomass in banana under in vitro conditions [ 33 ]. Furthermore, after 10 weeks of in vitro culture of Lamprocapnos spectabilis , treatment of AuNPs increased the shoot number, but reduced rooting [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

“…This suggests that the nanoparticles induced cellular metabolism in a positive manner, which subsequently led to growth enhancement of the plant. An increase in the chlorophyll content and total soluble sugar was observed in in vitro grown banana under Caralluma tuberculata AuNPs (Ca-AuNPs) and polyethylene glycol methacrylate coated Ca-AuNP nanocomposites (PEGMA-AuNPs) [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

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Charged Gold Nanoparticles Promote In Vitro Proliferation in Nardostachys jatamansi by Differentially Regulating Chlorophyll Content, Hormone Concentration, and Antioxidant Activity

et al. 2022

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Nardostachys jatamansi is a critically endangered medicinal plant and endemic to the Himalayas, having high commercial demand globally. The accumulation of various secondary metabolites in its shoots and roots with antioxidant potential are well-documented in traditional as well as modern medicine systems. In the present study, we first attempted to investigate the impact of citrate (−ve charge, 11.1 ± 1.9 nm) and CTAB (+ve charge, 19.5 ± 3.2 nm) coated gold nanoparticles (AuNPs) on the in vitro proliferation and antioxidant activities of N. jatamansi. Both the nanoparticles differentially affected the morphological and biochemical parameters, chlorophyll content, internal hormone concentration, and antioxidant activities in a concentration-dependent (10–100 µM) manner. Vigorous shooting was observed in half strength MS medium supplemented with IAA (1 mg/L) with 60 µM citrate-AuNPs (46.4 ± 3.7 mm) and 40 µM CTAB-AuNPs (42.2 ± 3.2 mm). Similarly, the maximum number of roots (5.00 ± 0.67 and 5.33 ± 0.58) and root length (29.9 ± 1.5 mm and 27.3 ± 4.8 mm) was reported in half-strength MS medium with IAA (1 mg/L) supplemented with 60 µM citrate-AuNPs and 40 µM CTAB-AuNPs, respectively. In addition, plants growing on MS medium supplemented with 60 µM citrate-AuNPs and 40 µM CTAB-AuNPs showed significantly enhanced photosynthetic pigments (chlorophyll a and b, carotenoids, and total chlorophyll), internal hormone concentration (GA3, IAA, and ABA), and antioxidant activities (total phenolics, flavonoids, DPPH, and SOD enzyme activity). Moreover, the transcript analysis of ANR1, ARF18, PLY9, SAUR28, GID1A, GRF1, SOD, and CAT further confirmed the role of 60 µM citrate-AuNPs and 40 µM CTAB-AuNPs in the improvement in the growth and antioxidant activities of N. jatamansi. Bearing in mind the urgent requirements of the effective conservation measures of this endangered species, the present findings suggest the elicitation of citrate-AuNPs and CTAB-AuNPs would significantly improve the potential applications of N. jatamansi in the medicinal plant-based industry.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Charged Gold Nanoparticles Promote In Vitro Proliferation in Nardostachys jatamansi by Differentially Regulating Chlorophyll Content, Hormone Concentration, and Antioxidant Activity

et al. 2022

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“…Silver nanoparticles are also used for the detection of biological molecules and possess catalytic properties [ 8 , 9 , 10 ]. Nanoparticles are known for their feasible production of biomass and natural antioxidants through plant tissue cultures, along with their role in reducing contamination and inducing the bioactive secondary metabolites of plants [ 11 , 12 , 13 ]. It has been proven that metal nanoparticles are highly reactive toward plants: due to their small size, they can easily penetrate the cell membrane and accumulate inside the cell.…”

Section: Introductionmentioning

confidence: 99%

Biological Synthesis of Silver Nanoparticles by Amaryllis vittata (L.) Herit: From Antimicrobial to Biomedical Applications

et al. 2022

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The current study sought to synthesize silver nanoparticles (AgNPs) from Amaryllis vittata (L.) leaf and bulb extracts in order to determine their biological significance and use the toxic plants for human health benefits. The formation of silver nanoparticles was detected by a change in color from whitish to brown for bulb-AgNPs and from light green to dark brown for leaf-AgNPs. For the optimization of silver nanoparticles, various experimental physicochemical parameters such as pH, temperature, and salt were determined. UV-vis spectroscopy, Fourier transform infrared spectroscopy, X-ray dispersion spectroscopy, scanning electron microscopy, and energy dispersion spectroscopy analysis were used to characterize nanoparticles. Despite the fact that flavonoids in plant extracts were implicated in the reduction and capping procedure, the prepared nanoparticles demonstrated maximum absorbency between 400 and 500 nm. SEM analysis confirmed the preparation of monodispersed spherical crystalline particles with fcc structure. The bioinspired nanoparticles were found to show effective insecticidal activity against Tribolium castaneum and phytotoxic activity against Lemna aequincotialis. In comparison to plant extracts alone, the tested fabricated nanoparticles showed significant potential to scavenge free radicals and relieve pain. Antibacterial testing against human pathogenic strains, i.e., Escherichia coli and Pseudomonas aureginosa, and antifungal testing against Aspergillus niger revealed the significant potential for microbe resistance using AgNPs. As a result of the findings, the tested silver nanoparticles demonstrated promising potential for developing new and effective pharmacological and agricultural medications. Furthermore, the effects of biogenic AgNPs on an in vitro culture of Solanum tuberosum L. plants were investigated, and the findings indicated that bulb-AgNPs and leaf-AgNPs produced biomass and induced antioxidants via their active constituents. As a result, bulb-AgNPs and leaf-AgNPs may be recommended for use in Solanum tuberosum L. tissue culture for biomass fabrication and metabolic induction.

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“…The solution of nanoparticles and polymer capped were dropped onto copper grids to prepare specimens for transmission electron microscopic (TEM) observation which was performed in a JEM 2100F with a field-emission gun operating at 200 kV. Dynamic light scattering (DLS) study was done using a model BI-200SM instrument (Brookhaven Instrument Corporation) ( Anwar et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

Hybridization of green synthesized silver nanoparticles with poly(ethylene glycol) methacrylate and their biomedical applications

Anwar

Khan

Shah

et al. 2022

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In the present research, a rapid, simple and efficient green method is used for the incorporation of silver nanoparticles (AgNPs) into poly(ethylene glycol) methacrylate (PEGMA) to create biocatalysts with excellent properties for pharmaceutical purpose. In the first phase, Caralluma tuberculata capped AgNPs (Ca-AgNPs) were prepared using green synthetic approach and in the second phase Caralluma tuberculata capped AgNPs were hybridized with poly(ethylene glycol) methacrylate to form PEGMA-AgNPs. Both the virgin (naked or uncapped) and polymer-capped materials were characterized spectroscopically and their results were compared. Fourier transform infrared spectroscopy showed no new peak after the capping procedure, showing that only physical interactions takes place during capping. After PEGMA capping, the spectra of the AgNPs red shifted (from 450 nm to 520 nm) and the overall particle size of AgNPs increased. Catalytic activity of the nanoparticles and hybrid system were tested by choosing the catalytic reduction of 4-nitrophenol (4-NP) as a model reaction. Both synthesized NPs and polymer capped NPs exhibits catalytic activity for the reduction of 4-NP to 4-aminophenol. The polymer hybrid exhibits remarkable antiproliferative, antioxidant, cytotoxic, antidiabetic and antileishmanial activities.

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Phytosynthesis of poly (ethylene glycol) methacrylate-hybridized gold nanoparticles from C. tuberculata: their structural characterization and potential for in vitro growth in banana

Cited by 17 publications

References 54 publications

Charged Gold Nanoparticles Promote In Vitro Proliferation in Nardostachys jatamansi by Differentially Regulating Chlorophyll Content, Hormone Concentration, and Antioxidant Activity

Charged Gold Nanoparticles Promote In Vitro Proliferation in Nardostachys jatamansi by Differentially Regulating Chlorophyll Content, Hormone Concentration, and Antioxidant Activity

Biological Synthesis of Silver Nanoparticles by Amaryllis vittata (L.) Herit: From Antimicrobial to Biomedical Applications

Hybridization of green synthesized silver nanoparticles with poly(ethylene glycol) methacrylate and their biomedical applications

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