Phytosynthesis of CuONPs via Laurus nobilis: Determination of antioxidant content, antibacterial activity, and dye decolorization potential

Abstract

Copper oxide nanoparticles (CuONPs) were phytosynthesized by Laurus nobilis leaf extract, which was used as a reducing and capping agent. UV‐vis spectroscopy was applied, and the spectrum of CuONPs gave a peak around 300 and 325 nm. An intense Fourier transform infrared spectroscopy between 4000 and 500 cm−1 wavelengths exhibited exterior functional groups of CuONPs. The results of scanning electron microscopy and transmission electron microscopy revealed that the green synthesized CuONPs were spherical in shape with sizes between 90 and 250 nm. Antibacterial activity of CuONPs was evaluated against both Gram‐positive and Gram‐negative bacteria. Brilliant Blue R‐250 was employed in the dye decolorization studies, and CuONPs achieved 69% decolorization in 60 Min. The antioxidant activity of CuONPs was calculated by analyzing total phenolic compounds and flavonoid content. Furthermore, the reducing power of extract and nanoparticles was determined. Total phenolic compounds of CuONPs were determined as 6.7 µg of pyrocatechol equivalent/mg, while total flavonoids were measured as 236.62 µg catechin/mg sample. Results indicated that the method of CuONP formation is simple and low cost and the phytosynthesized CuONPs had antibacterial, antioxidant, and dye decolorization activity.

Isolation of Laurus nobilis Leaf Polyphenols: A Review on Current Techniques and Future Perspectives

“…Ramos et al [ 62 ] obtained higher TPC in boiling water after 3 h, than at room temperature after 3 days. Moreover, the TPC of water extraction at 80 °C for 45 min [ 56 ] was 10-fold higher when compared to TPC obtained at room temperature during 24 h [ 62 ]. Extraction temperature and time are two significantly linked parameters, where extraction at lower temperatures requires a longer extraction time, while shorter extraction time is achieved when using moderate or high temperatures of extraction [ 52 ].…”

Anti-Helicobacter pylori, Antioxidant, Antidiabetic, and Anti-Alzheimer’s Activities of Laurel Leaf Extract Treated by Moist Heat and Molecular Docking of Its Flavonoid Constituent, Naringenin, against Acetylcholinesterase and Butyrylcholinesterase

“…Higher total phenolic content was obtained as a result of exposure of L. nobilis to boiling water for 3 h, compared to the amount obtained at room temperature after 72 h [37]. In another report, Bulut Kocabas et al [38] noticed that the total phenolic content of L. nobilis leaves exposed to heated water at 80 • C for 45 min was 10-fold more than that obtained at room temperature for 24 h, with apigenin and its glycosides representing the most detected flavones in the LLE [39]. But in our study, apigenin was detected at a low concentration.…”

Synthesis of copper‐oxide nanoparticles from Euphorbia antiquorum extract and evaluation of its dye degradation capacity

“…In earlier studies also, CuONPs were also extracted from various plants including Azadirachta indica leaf extract against reactive red 120 dye (Kandi et al., 2017), CuONPs from Vitex negundo Linn leaf extract against MB, CBB, and CRdyes (Bhavyasree & Xavier, 2021). CuONPs from Laurus nobilis leaf extract against Brilliant Blue R‐250 (Bulut Kocabas et al., 2021), CuONPs from Psidium guajava leaf extract against Navy blue and RY160 (Singh et al., 2019), and CuO NPs from Drypetes sepiaria leaf extract against Congo Red. UV–visible spectroscopy can be used to investigate the material's physicochemical properties and surface morphology (Bhavyasree & Xavier, 2021).…”