Phytosulfokine-α, a sulfated pentapeptide, stimulates the proliferation of rice cells by means of specific high- and low-affinity binding sites

Matsubayashi, Yoshikatsu; Takagi, Leiko; Sakagami, Youji

doi:10.1073/pnas.94.24.13357

Cited by 131 publications

(91 citation statements)

References 28 publications

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“…The only protoplast cultures, which have been shown to respond to PSK with enhanced proliferation activity, were derived from cell suspensions of rice (Matsubayashi et al 1997). In this paper we report a very high positive impact of PSK on otherwise extremely recalcitrant sugar beet leaf protoplast culture.…”

Section: Introductionmentioning

confidence: 68%

Phytosulfokine stimulates cell divisions in sugar beet (Beta vulgaris L.) mesophyll protoplast cultures

et al. 2012

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The aim of this work was to improve plating efficiency of sugar beet mesophyll protoplast cultures. Preliminary experiments showed that cultures of good quality, viable protoplasts were obtained in rich media based on the Kao and Michayluk formulation and with the calcium alginate as an embedding matrix. Nevertheless, in these cultures cell divisions were either not observed or very seldom confirming earlier reported recalcitrance of sugar beet protoplasts. The recalcitrant status of these cultures was reversed upon application of exogenous phytosulfokine (PSK)-a peptidyl plant growth factor. The highest effectiveness of PSK was observed at 100 nM concentration. Plating efficiencies obtained in the presence of PSK reached approximately 20% of the total cultured cells. The stimulatory effect of phytosulfokine was observed for all tested breeding stocks of sugar beet. Our data indicate that PSK is a powerful agent able to overcome recalcitrance of plant protoplast cultures.

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Section: Introductionmentioning

confidence: 68%

Phytosulfokine stimulates cell divisions in sugar beet (Beta vulgaris L.) mesophyll protoplast cultures

et al. 2012

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“…Subsequent studies demonstrated the existence of PSK-K in CM derived from both monocot [7] and dicot [8,9] cell cultures. Furthermore, isolation of a rice (Oryza sativa L.) cDNA, OsPSK, encoding a putative PSK-K precursor has been achieved [10] and it was also shown that rice suspension cells contain both speci¢c high-and low-a¤n-ity saturable PSK-K binding sites on their plasma membranes [11].…”

Section: Introductionmentioning

confidence: 99%

Existence of a plant tyrosylprotein sulfotransferase: novel plant enzyme catalyzing tyrosine O‐sulfation of preprophytosulfokine variants in vitro

et al. 2000

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An in vitro assay system to detect tyrosylprotein sulfotransferase (TPST) activity of higher plant cells was established, using synthetic oligopeptides based on the deduced amino acid sequence of a phytosulfokine-K K (PSK-K K) precursor. TPST activity was found in microsomal membrane fractions of rice, asparagus and carrot cells and it was confirmed that acidic amino acid residues adjacent to the tyrosine residues of acceptor peptides were essential to the sulfation reaction. The asparagus TPST exhibited a broad pH optimum of 7.0^8.5, required manganese ions for maximal activity and appeared to be a membrane-bound protein localized in the Golgi apparatus. These enzymes should be defined as a new class of plant sulfotransferases that catalyze tyrosine O-sulfation of a PSK-K K precursor and other unknown proteins.z 2000 Federation of European Biochemical Societies.

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“…Among them, the most important are growth regulators, particularly auxins and cytokinins, normally essential for sustained divisions of protoplast-derived cells and their further differentiation into plants (Davey et al 2005b). Recently, oligopeptides have been recognized as signaling molecules playing an important role in plant cell growth and development (Matsubayashi et al 1997;Ryan and Pearce 2001). One of them is phytosulfokine (PSK), a plant-specific disulfated pentapeptide isolated for the first time from conditioned medium of an Asparagus officinalis mesophyll cell culture (Matsubayashi and Sakagami 1996).…”

Section: Introductionmentioning

confidence: 99%

Plant regeneration from leaf-derived protoplasts within the Daucus genus: effect of different conditions in alginate embedding and phytosulfokine application

Maćkowska

Jarosz

Grzebelus

2014

Plant Cell Tiss Organ Cult

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Protoplasts of four Daucus carota subspecies and three wild Daucus species were isolated from 2-weekold shoot cultures during overnight incubation in an enzyme mixture composed of 1 % (w/v) cellulase Onozuka R-10 and 0.1 % (w/v) pectolyase Y-23. Before the culture, they were embedded in autoclave-or filter-sterilized alginate solution. Modified thin alginate layer (TAL) and extra thin alginate film (ETAF) techniques were applied for protoplast immobilization. A rich mineral-organic medium based on the formulation of Kao and Michayluk supplemented with 0.1 mg l -1 2,4-dichlorophenoxyacetic acid, 0.2 mg l -1 zeatin, and optionally 100 nM phytosulfokine (PSK), a peptidyl plant growth factor, was used for protoplast culture. Plating efficiency was genotype-dependent and in 40-day-old cultures, it varied from 10 % for Daucus pusillus to 73 % for D. carota subsp. sativus. A considerably higher ability in colony formation was observed in the modified TAL culture system using filter-sterilized alginate and in the presence of PSK in the protoplast culture medium. Plant regeneration through somatic embryogenesis stimulated by PSK application occurred for five out of the seven Daucus accessions used in the present study. We believe our data may facilitate the use of wild Daucus in somatic hybridization with cultivated carrot.

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Phytosulfokine-α, a sulfated pentapeptide, stimulates the proliferation of rice cells by means of specific high- and low-affinity binding sites

Abstract: Peptide growth factors were isolated from conditioned medium derived from rice (Oryza sativa L.) suspension cultures and identified to be a sulfated pentapeptide

Cited by 131 publications

References 28 publications

Phytosulfokine stimulates cell divisions in sugar beet (Beta vulgaris L.) mesophyll protoplast cultures

Phytosulfokine stimulates cell divisions in sugar beet (Beta vulgaris L.) mesophyll protoplast cultures

Existence of a plant tyrosylprotein sulfotransferase: novel plant enzyme catalyzing tyrosine O‐sulfation of preprophytosulfokine variants in vitro

Plant regeneration from leaf-derived protoplasts within the Daucus genus: effect of different conditions in alginate embedding and phytosulfokine application

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