Phytostimulatory effect of silver nanoparticles (AgNPs) on rice seedling growth: An insight from antioxidative enzyme activities and gene expression patterns

Gupta, S. Dutta; Agarwal, Avinash; Pradhan, Subrata

doi:10.1016/j.ecoenv.2018.06.023

Cited by 196 publications

(101 citation statements)

References 73 publications

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“…This observation was coherent with the existing literature showing that the SOD activity was not affected by similar size AgNPs 19 or different size AgNPs on the activity of recombinant SOD 14 and the SOD in seeds of rice Oryza sativa. 49 The lack of AgNP effects on the SOD activity could be related with the enzyme structure. The two subunits of SOD were reported to be tightly joined by hydrophobic and electrostatic forces.…”

Section: View Article Onlinementioning

confidence: 99%

Interaction of silver nanoparticles with antioxidant enzymes

Liu

Worms

Slaveykova

2020

Environ. Sci.: Nano

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Oxidative stress is accepted as a key mechanism of silver nanoparticle (AgNP) toxicity in living organisms. Therefore, mechanistic studies related to the impact of AgNPs on the structure and function of antioxidant enzymes at the molecular level are essential for a comprehensive evaluation of their toxicity. By using a combination of spectroscopic, imaging and fractionation techniques, we explored the interactions between citrate-coated AgNPs (20 nm) and two key antioxidant enzymes: catalase (CAT) and superoxide dismutase (SOD). Both enzymes interacted with AgNPs by forming surface complexes. Only CAT was able to promote AgNP dissolution, without the impact of the released Ag ions on its heme cofactor. Instead, our results suggest that the formation of the AgNP-CAT complex induced conformation changes in the CAT, which resulted in an impairment of its enzymatic activity together with AgĲI) adsorption. By contrast, the formation of the AgNP-SOD complex has only a marginal influence on the protein conformation and has no impact on its metallic cofactors, and thus its enzymatic activity. Overall, the results showed that the changes in the protein conformation and the dissolution of AgNPs depended on the protein structure and could result in different degrees of enzymatic activity modulation.Excessive production of reactive oxygen species in cells by direct interaction with particles and/or dissolved species is currently accepted as one of the main mechanisms of cellular toxicity of engineered nanoparticles. Therefore, studies related to the impact of silver nanoparticles (AgNPs) on the structure and function of antioxidant enzymes at the molecular level are crucial for a comprehensive evaluation of their toxicity. This study suggests that the conformation changes of two key antioxidant enzymes, catalase and superoxide dismutase, and the dissolution of AgNPs depended on the protein structure and could result in different degrees of enzymatic activity modulation. This research provides a basis to understand how the functional properties of the key antioxidant enzymes may affect the stability of nanoparticles in a biological context and give insights into the role of these enzymes in the mechanism of NP-induced redox stress in vivo.

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Section: View Article Onlinementioning

confidence: 99%

Interaction of silver nanoparticles with antioxidant enzymes

Liu

Worms

Slaveykova

2020

Environ. Sci.: Nano

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“…They affect plants at many different levels [13,14]. Positive effects of AgNPs include stimulation of germination [15], growth invigoration [16], increased biomass accumulation [17], improved shoot induction and proliferation [7], or enhanced pigment content [18]. Silver nanoparticles may also show phytotoxicity, manifested by limited germination and seedling growth [19], decreased biomass of leaves and shoots [20], delay in flowering time, and inhibition of photosynthesis [21].…”

Section: Introductionmentioning

confidence: 99%

Stimulatory Effect of Silver Nanoparticles on the Growth and Flowering of Potted Oriental Lilies

et al. 2019

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Nanoparticles exhibit unique biological activities and may serve as novel plant growth stimulators. This research consisted of a two-year pot experiment designed to find out if silver nanoparticles (AgNPs) might be used in the cultivation of Oriental lilies. In the first year, we evaluated the effects of various concentrations of AgNPs (0, 25, 50, 100, and 150 ppm) and their application methods (pre-planting bulb soaks, foliar sprays, and substrate drenches) on the growth and flowering of Lilium cv. Mona Lisa. In the second year, we evaluated the effects of soaking the bulbs of cv. Little John in the same concentration of AgNP solution on plant morphological features, leaf content of photosynthetic pigments, basic macronutrients, and complex biomolecules with the use of the Fourier-transform infrared spectroscopy (FTIR). Soaking the bulbs in a nanoparticle solution turned out to be the most effective strategy for growth and flowering promotion. AgNPs stimulated plant growth, as manifested by enhanced accumulation of leaf and bulb biomass and accelerated flowering. Moreover, plants treated with silver nanoparticles showed higher leaf greenness index, formed more flowers, and flowered longer. At 100 ppm AgNPs, the leaves accumulated the highest content of chlorophyll a, chlorophyll b, and carotenoids, and were the richest in potassium, calcium, and sulfur. The FTIR spectra did not show any changes in absorbance intensity and chemical composition in the leaves from AgNP-treated bulbs.

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“…Physiological responses are the first key changes, which occur when plants are exposed to elements of diverse nature. NPs trigger plant physiological response in both positive and negative ways [17,18], primarily depending on the concentration of NPs and plant species [19,20]. Generally, application of NPs in lower concentrations has been found promoting plant growth and secondary metabolites production [21].…”

Section: Resultsmentioning

confidence: 99%

Biogenic zinc oxide nanoparticles-enhanced biosynthesis of lignans and neolignans in cell suspension cultures of Linum usitatissimum L

Abbasi

Zahir

Ahmad

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Zinc oxide nanoparticles (NPs) have emerged as a novel elicitor for enhanced biosynthesis of secondary metabolites in in vitro plant cell cultures. The current study was aimed to explore elicitation abilities of ZnO-NPs for enhanced accumulation of lignans and neolignans in cell cultures of Linum usitatissimum. We optimized concentration of zinc oxide NPs before carrying out a full-fledged experiment. Subsequently, an optimum dose of 100 mg/l was introduced into the culture medium on day 0, days 0 and 15, and finally days 0 and 25. We observed that repeated elicitation stimulated various parameters and physiological responses in Linum usitatissimum cell cultures than one-time elicitation. Repeated elicitation of cell cultures on day 0 and 15 resulted in highest fresh weight (412.16 g/l) and lignans production (secoisolariciresinol diglucoside 284.12 mg/l: lariciresinol diglucoside 86.97 mg/l). Contrarily, repeated elicitation on day 0 and 25 resulted in highest DW (13.53 g/l), total phenolic production (537.44 mg/l), total flavonoid production (123.83 mg/l) and neolignans production (dehydrodiconiferyl alcohol glucoside 493.28 mg/l: guaiacylglycerol-b-coniferyl alcohol ether glucoside 307.69 mg/l). Enhancement in plant growth and secondary metabolites accumulation was several fold higher than controls. Furthermore, a linear relationship existed between total phenolic and flavonoid contents which in turn was correlated with higher antioxidant activities. ARTICLE HISTORY

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Phytostimulatory effect of silver nanoparticles (AgNPs) on rice seedling growth: An insight from antioxidative enzyme activities and gene expression patterns

Cited by 196 publications

References 73 publications

Interaction of silver nanoparticles with antioxidant enzymes

Interaction of silver nanoparticles with antioxidant enzymes

Stimulatory Effect of Silver Nanoparticles on the Growth and Flowering of Potted Oriental Lilies

Biogenic zinc oxide nanoparticles-enhanced biosynthesis of lignans and neolignans in cell suspension cultures of Linum usitatissimum L

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