Phytostimulation and biocontrol potential of Gram-positive endospore-forming Bacilli

Soni, Riteshri; Keharia, Haresh

doi:10.1007/s00425-021-03695-0

Cited by 26 publications

(16 citation statements)

References 234 publications

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“…Lysinibacillus and Paenibacillus were upregulated by malic acid. These bacilli are reported to be probiotics and considered as potential fermentation additives ( Liñan-Vidriales et al, 2021 ; Soni and Keharia, 2021 ). Methylobacterium is a strictly aerobic bacterium and involved in environmental carbon cycle and decomposition of compounds in plants ( Wang et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Additives Altered Bacterial Communities and Metabolic Profiles in Silage Hybrid Pennisetum

et al. 2022

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This study was conducted to investigate the effects of different additives on the fermentation quality, nutrient composition, bacterial communities, and metabolic profiles of the silage of hybrid Pennisetum. The experiment was conducted using five treatments, i.e., CK, control group, MA, 1% malic acid of fresh matter (FM) basis, GL, 1% glucose of FM basis, CE, 100 U/g FM cellulase, and BS, 106 cfu/g FM Bacillus subtilis, with six replicates each treatment. After a 120-day fermentation, 30 silage packages were opened for subsequent determination. As a result, all four additives had positive effects on the fermentation quality and nutrient composition of the silage of hybrid Pennisetum. The high-throughput sequencing of V3–V4 regions in 16S rRNA was performed, and results showed that Firmicutes and Proteobacteria were the dominant phyla and that Aquabacterium and Bacillus were the dominant genera. MA, GL, CE, and BS treatment resulted in 129, 21, 25, and 40 differential bacteria, respectively. The four additives upregulated Bacillus smithii but downregulated Lactobacillus rossiae. Metabolic profiles were determined by UHPLC-Q/TOF-MS technology and the differential metabolites caused by the four additives were 47, 13, 47, and 18, respectively. These metabolites played antioxidant, antibacterial, and anti-inflammatory functions and involved in pathways, such as the citrate cycle, carbon fixation in photosynthetic organisms, and glyoxylate and dicarboxylate metabolism. In conclusion, silage additives promoted fermentation quality and nutrient composition by altering bacterial communities and metabolic profiles. This study provided potential biomarkers for the improvement of silage quality.

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Section: Discussionmentioning

confidence: 99%

Additives Altered Bacterial Communities and Metabolic Profiles in Silage Hybrid Pennisetum

et al. 2022

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“…Many rhizobacteria play growth promotion and biological control functions for plants and are called plant growth-promoting rhizobacteria (PGPR) [ 1 – 3 ]. Paenibacillus polymyxa is a typical PGPR that can promote the growth, development, and stress resistance of plants [ 4 – 6 ].…”

Section: Introductionmentioning

confidence: 99%

Identification and combinatorial engineering of indole-3-acetic acid synthetic pathways in Paenibacillus polymyxa

Sun

Zhang

Liu

et al. 2022

Biotechnol Biofuels

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Background Paenibacillus polymyxa is a typical plant growth-promoting rhizobacterium (PGPR), and synthesis of indole-3-acetic acid (IAA) is one of the reasons for its growth-promoting capacity. The synthetic pathways of IAA in P. polymyxa must be identified and modified. Results P. polymyxa SC2 and its spontaneous mutant SC2-M1 could promote plant growth by directly secreting IAA. Through metabonomic and genomic analysis, the genes patA, ilvB3, and fusE in the native IPyA pathway of IAA synthesis in strain SC2-M1 were predicted. A novel strong promoter P04420 was rationally selected, synthetically analyzed, and then evaluated on its ability to express IAA synthetic genes. Co-expression of three genes, patA, ilvB3, and fusE, increased IAA yield by 60% in strain SC2-M1. Furthermore, the heterogeneous gene iaam of the IAM pathway and two heterogeneous IPyA pathways of IAA synthesis were selected to improve the IAA yield of strain SC2-M1. The genes ELJP6_14505, ipdC, and ELJP6_00725 of the entire IPyA pathway from Enterobacter ludwigii JP6 were expressed well by promoter P04420 in strain SC2-M1 and increased IAA yield in the engineered strain SC2-M1 from 13 to 31 μg/mL, which was an increase of 138%. Conclusions The results of our study help reveal and enhance the IAA synthesis pathways of P. polymyxa and its future application.

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“…Bacillus spp. can secrete many secondary metabolites including lipopeptides (surfactin, fengycin and iturin), polyketides (difficidin, bacillaene and macrolactin) and volatiles, which may directly inhibit the growth or sporulation of plant pathogens or elicit induced systemic resistance (ISR) of the plant host ( Ongena and Jacques, 2008 ; Soni and Keharia, 2021 ). Surfactin or biosurfactant, a lipoheptapeptide, was first isolated in 1968 from B. subtilis ( Arima et al., 1968 ), and its synthesis required multiple enzymes called nonribosomal peptide synthetases ( Theatre et al., 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Surfactin secreted by Bacillus amyloliquefaciens Ba01 is required to combat Streptomyces scabies causing potato common scab

Feng

Chen²,

Lin³

et al. 2022

Front. Plant Sci.

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Potato common scab, which is mainly caused by the bacterium Streptomyces scabies, occurs in key potato growing regions worldwide. It causes necrotic or corky symptoms on potato tubers and decreases the economic value of potato. At present, there is no recommended chemical or biological control for combating potato common scab in Taiwan. It can only reduce the occurrence by cultivation control, but the efficacy is limited. Previously we found that Bacillus amyloliquefaciens Ba01 could control potato common scab in pot assay and in the field. The potential anti-S. scabies mechanism was associated with surfactin secretion, but further molecular dissection was not conducted. Thus, in this study we aimed to determine whether surfactin is the main compound active against S. scabies by knocking out the srf gene cluster in Ba01. The cloning plasmid pRY1 was transformed to Ba01 by electroporation for in-frame deletion. Two independent Δsrf mutants were obtained and confirmed by specific primers and mass spectrometry. The swarming ability and S. scabies inhibition was significantly decreased (P<0.001) in Δsrf mutants. The swarming ability of Δsrf mutants could be restored by the addition of surfactin. Furthermore, we found that Ba01 formed wrinkled biofilm in MSgg liquid medium, while Δsrf mutants formed biofilm abnormally. Furthermore, the α-amylase, protease and phosphate-solubilizing ability of Δsrf mutants was decreased, and the mutants could not inhibit the growth and sporulation of S. scabies on potato tuber slices. In conclusion, srf gene cluster of B. amyloliquefaciens Ba01 is responsible for the secretion of surfactin and inhibition of S. scabies.

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Phytostimulation and biocontrol potential of Gram-positive endospore-forming Bacilli

Cited by 26 publications

References 234 publications

Additives Altered Bacterial Communities and Metabolic Profiles in Silage Hybrid Pennisetum

Additives Altered Bacterial Communities and Metabolic Profiles in Silage Hybrid Pennisetum

Identification and combinatorial engineering of indole-3-acetic acid synthetic pathways in Paenibacillus polymyxa

Surfactin secreted by Bacillus amyloliquefaciens Ba01 is required to combat Streptomyces scabies causing potato common scab

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