Phytosterol Pygeum africanum regulates prostate cancer in vitro and in vivo

Shenouda, Nader S.; Sakla, Mary S.; Newton, L. G.; Besch‐Williford, Cynthia; Greenberg, Norman M.; MacDonald, Ruth S.; Lubahn, Dennis B.

doi:10.1007/s12020-007-0014-y

Cited by 54 publications

(60 citation statements)

References 43 publications

(61 reference statements)

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“…Male TRAMP mice (10) on a C57BL6/J background were raised in-house as described previously (11). The mice were fed the specific diets from 5 wk until 5 mo of age (18-22 mice per treatment group).…”

Section: Methodsmentioning

confidence: 99%

Common Botanical Compounds Inhibit the Hedgehog Signaling Pathway in Prostate Cancer

et al. 2010

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Many botanical compounds have been proposed to prevent cancer. We investigated the cancer treatment and prevention abilities of apigenin, baicalein, curcumin, epigallocatechin 3-gallate (EGCG), genistein, quercetin, and resveratrol both in vivo in transgenic adenocarcinoma of the mouse prostate (TRAMP) mice as well as in vitro in prostate cancer cell lines. In our experiments, these seven compounds act similarly to the Hedgehog antagonist cyclopamine, a teratogenic plant alkaloid, which had been previously shown to "cure" prostate cancer in a mouse xenograft model. With IC 50 values ranging from <1 to 25 μmol/L, these compounds can inhibit Gli1 mRNA concentration by up to 95% and downregulate Gli reporter activity by 80%. We show that four compounds, genistein, curcumin, EGCG, and resveratrol, inhibit Hedgehog signaling as monitored by real-time reverse transcription-PCR analysis of Gli1 mRNA concentration or by Gli reporter activity. Three compounds, apigenin, baicalein, and quercetin, decreased Gli1 mRNA concentration but not Gli reporter activity. Our results show that these compounds are also able to reduce or delay prostate cancer in vivo in TRAMP mice. All seven compounds, when fed in combination as pure compounds or as crude plant extracts, inhibit well-differentiated carcinoma of the prostate by 58% and 81%, respectively. In vitro, we show that all seven compounds also inhibit growth in human and mouse prostate cancer cell lines. Mechanistically, we propose the Hedgehog signaling pathway to be a direct or indirect target of these compounds. These botanicals at pharmacologic concentrations are potentially safer and less expensive alternatives to cyclopamine and its pharmaceutical analogues for cancer therapy. Cancer Res; 70(8); 3382-90. ©2010 AACR.

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Section: Methodsmentioning

confidence: 99%

Common Botanical Compounds Inhibit the Hedgehog Signaling Pathway in Prostate Cancer

et al. 2010

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“…TRAMP mice, a mouse model to investigate PCa, exhibited a reduction of PCa incidence compared to the control group after feeding with Pygeum africanum extract (Shenouda et al, 2007).…”

Section: Roell and Baniahmadmentioning

confidence: 98%

“…Herby, the pulverized bark is incorporated into capsules and commercially available as the remedy Tadenan ® and in other European countries under various different trade names (Ishani et al, 2000). In the USA phytotherapeutic agents like extracts of Pygeum africanum have been marketed for prostate health including PCa prevention and treatment (Shenouda et al, 2007).…”

Section: Roell and Baniahmadmentioning

confidence: 99%

“…Recently, the ethanolic extracts of Pygeum africanum were shown to inhibit human PCa cell growth, induce apoptosis and alter cell kinetics (Shenouda et al, 2007). TRAMP mice, a mouse model to investigate PCa, exhibited a reduction of PCa incidence compared to the control group after feeding with Pygeum africanum extract (Shenouda et al, 2007).…”

Section: Roell and Baniahmadmentioning

confidence: 99%

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The natural compounds atraric acid and N-butylbenzene-sulfonamide as antagonists of the human androgen receptor and inhibitors of prostate cancer cell growth

Roell

Baniahmad

2011

Molecular and Cellular Endocrinology

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International audienceExtracts from the plant are widely used in the therapy of benign prostate hyperplasia (BPH) and in combinational therapy for prostate cancer, the second leading cause of cancer death and the mostly diagnosed form of cancer in men. The androgen receptor (AR) plays a crucial role in the development of the prostate as well as in prostate diseases. Even though the extracts from are considered as beneficial for prostate diseases in clinical trials, and some active compounds for treatment of BPH could be identified, compounds responsible for AR inhibition and the molecular mechanism for inhibition of prostatitis need to be identified. Recently, atraric acid and N-butylbenzene-sulfonamide were isolated from a selective dichlormethane extract of as two novel AR antagonistic compounds. The molecular mechanisms of AR inhibition were analyzed and are summarized here. Both compounds are the first known natural, complete and specific AR antagonist

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“…TRAMP mice fed with P. africanum showed a significant reduction (P = 0.034) in prostate cancer incidence (35%) compared to casein fed mice (62.5%). P. africanum therefore has a significant role in regulation of prostate cancer both in vitro and in vivo and therefore may be a useful supplement for people at high risk for developing prostate cancer (Shenouda et al, 2007). Katz (2002) had observed that the consumption of isoflavones found in legumes and other plants is related to lower rates of BPH and prostate cancer among Asian men (Katz, 2002).…”

Section: Figure 6: Stages In Cancer Prevention Researchmentioning

confidence: 99%

The role and place of medicinal plants in the strategies for disease prevention

Sofowora

Ogunbodede

Onayade

2013

Afr. J. Trad. Compl. Alt. Med.

594

428

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Medicinal plants have been used in healthcare since time immemorial. Studies have been carried out globally to verify their efficacy and some of the findings have led to the production of plant-based medicines. The global market value of medicinal plant products exceeds $100 billion per annum. This paper discusses the role, contributions and usefulness of medicinal plants in tackling the diseases of public health importance, with particular emphasis on the current strategic approaches to disease prevention. A comparison is drawn between the 'whole population' and 'high-risk' strategies. The usefulness of the common-factor approach as a method of engaging other health promoters in propagating the ideals of medicinal plants is highlighted. The place of medicinal plants in preventing common diseases is further examined under the five core principles of the Primary Health Care (PHC) approach. Medicinal plants play vital roles in disease prevention and their promotion and use fit into all existing prevention strategies. However, conscious efforts need to be made to properly identify, recognise and position medicinal plants in the design and implementation of these strategies. These approaches present interesting and emerging perspectives in the field of medicinal plants. Recommendations are proposed for strategising the future role and place for medicinal plants in disease prevention.

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Phytosterol Pygeum africanum regulates prostate cancer in vitro and in vivo

Abstract: Background

Cited by 54 publications

References 43 publications

Common Botanical Compounds Inhibit the Hedgehog Signaling Pathway in Prostate Cancer

Common Botanical Compounds Inhibit the Hedgehog Signaling Pathway in Prostate Cancer

The natural compounds atraric acid and N-butylbenzene-sulfonamide as antagonists of the human androgen receptor and inhibitors of prostate cancer cell growth

The role and place of medicinal plants in the strategies for disease prevention

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