Three new phytoseiid species of the subfamily Amblyseiinae, Neoseiulus laetus sp. nov., N. uncinatus sp. nov. and Chelaseius longicervix sp. nov., and the male of Neoseiulus madeirensis Papadoulis & Kapaxidi, are described from the Madeira archipelago. Moreover, six Amblyseiinae species are also reported for the first time from the islands and additional morphological information is provided for Euseius machadoi Ferragut & Peña-Estévez, with a discussion on the variability between Madeiran and Canarian populations.