Phytoplankton photosynthetic rate measurement using tunable pulsed light induced fluorescence kinetics

Yin, Guisheng; Zhao, Nanjing; Shi, Chaoyi; Chen, Shuang; Qin, Zhisong; Yan, Ruifang; Gan, Tingting; Liu, Jianguo; Liu, Wenqing

doi:10.1364/oe.26.00a293

Cited by 13 publications

(4 citation statements)

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“…The photosynthesis rate of the Arabidopsis plants of different lines after drought treatment was analyzed using the Portable Photosynthesis Rate Detector AGHJ-PPF (Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences) after a 30 min dark adaptation, as previously described [66]. Parameters including the maximum photochemical efficiency of PSII (Fv/Fm), minimal fluorescence (F0), maximal fluorescence (Fm), and PSII (as shown in Figure S1) were calculated according to a previous method [66].…”

Section: Methodsmentioning

confidence: 99%

Overexpression of the Stress-Inducible SsMAX2 Promotes Drought and Salt Resistance via the Regulation of Redox Homeostasis in Arabidopsis

Wang

Shah

et al. 2019

IJMS

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Recent studies have demonstrated that strigolactones (SLs) also participate in the regulation of stress adaptation; however, the regulatory mechanism remains elusive. In this study, the homolog of More Axillary Branches 2, which encodes a key component in SL signaling, in the perennial oil plant Sapium sebiferum was identified and functionally characterized in Arabidopsis. The results showed that the expression of SsMAX2 in S. sebiferum seedlings was stress-responsive, and SsMAX2 overexpression (OE) in Arabidopsis significantly promoted resistance to drought, osmotic, and salt stresses. Moreover, SsMAX2 OE lines exhibited decreased chlorophyll degradation, increased soluble sugar and proline accumulation, and lower water loss ratio in response to the stresses. Importantly, anthocyanin biosynthesis and the activities of several antioxidant enzymes, such as superoxide dismutase (SOD), peroxidase (POD), and ascorbate peroxidase (APX), were enhanced in the SsMAX2 OE lines, which further led to a significant reduction in hydrogen peroxide levels. Additionally, the SsMAX2 OE lines exhibited higher expression level of several abscisic acid (ABA) biosynthesis genes, suggesting potential interactions between SL and ABA in the regulation of stress adaptation. Overall, we provide physiological and biochemical evidence demonstrating the pivotal role of SsMAX2 in the regulation of osmotic, drought, and salt stress resistance and show that MAX2 can be a genetic target to improve stress tolerance.

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Section: Methodsmentioning

confidence: 99%

Overexpression of the Stress-Inducible SsMAX2 Promotes Drought and Salt Resistance via the Regulation of Redox Homeostasis in Arabidopsis

Wang

Shah

et al. 2019

IJMS

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“…The MDA, hydrogen peroxide (H 2 O 2 ), SOD, peroxidase (POD), CAT, and ascorbate peroxidase (APX) contents were determined by using an MDA assay kit, a plant soluble sugar content test kit, an H 2 O 2 assay kit, an SOD assay kit, a POD assay kit, a CAT assay kit, and an APX assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China), following a protocol provided with a specific kit, as previously described by Ni et al (2018b). The maximum photochemical efficiency of PSII (Fv/Fm) was calculated according to a previous method (Yin et al, 2018).…”

Section: Biochemical Analysis and Photochemical Efficiency Of The Psii Measurementmentioning

confidence: 99%

Karrikin Improves Osmotic and Salt Stress Tolerance via the Regulation of the Redox Homeostasis in the Oil Plant Sapium sebiferum

et al. 2020

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Karrikins are reported to stimulate seed germination, regulate seedling growth, and increase the seedling vigor in abiotic stress conditions in plants. Nevertheless, how karrikins alleviate abiotic stress remains largely elusive. In this study, we found that karrikin (KAR 1 ) could significantly alleviate both drought and salt stress in the important oil plant Sapium sebiferum. KAR 1 supplementation in growth medium at a nanomolar (nM) concentration was enough to recover seed germination under salt and osmotic stress conditions. One nanomolar of KAR 1 improved seedling biomass, increased the taproot length, and increased the number of lateral roots under abiotic stresses, suggesting that KAR 1 is a potent alleviator of abiotic stresses in plants. Under abiotic stresses, KAR 1 -treated seedlings had a higher activity of the key antioxidative enzymes, such as superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase, in comparison with the control, which leads to a lower level of hydrogen peroxide, malondialdehyde, and electrolyte leakage. Moreover, the metabolome analysis showed that KAR 1 treatment significantly increased the level of organic acids and amino acids, which played important roles in redox homeostasis under stresses, suggesting that karrikins might alleviate abiotic stresses via the regulation of redox homeostasis. Under abiotic stresses, applications of karrikins did not increase the endogenous abscisic acid level but altered the expression of several ABA signaling genes, such as SNF1-RELATED PROTEIN KINASE2.3, SNF1-RELATED PROTEIN KINASE2.6, ABI3, and ABI5, suggesting potential interactions between karrikins and ABA signaling in the stress responses. Conclusively, we not only provided the physiological and molecular evidence to clarify the mechanism of karrikins in the regulation of stress adaptation in S. sebiferum but also showed the potential value of karrikins in agricultural practices, which will lay a foundation for further studies about the role of karrikins in abiotic stress alleviation in plants.

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“…Yin et al [12] put forward a photosynthetic rate measurement method based on tunable pulsed light induced fluorescence kinetics on the basis of 'Bio-Optical' model. They used the chlorophyll fluorescence as the probe of photosynthesis process and evaluated the phytoplankton photosynthetic rate by the photosynthetic electron transport rate.…”

Section: Environment Monitoringmentioning

confidence: 99%

Feature issue introduction: Light, Energy and the Environment, 2016

et al. 2017

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Phytoplankton photosynthetic rate measurement using tunable pulsed light induced fluorescence kinetics

Cited by 13 publications

References 4 publications

Overexpression of the Stress-Inducible SsMAX2 Promotes Drought and Salt Resistance via the Regulation of Redox Homeostasis in Arabidopsis

Overexpression of the Stress-Inducible SsMAX2 Promotes Drought and Salt Resistance via the Regulation of Redox Homeostasis in Arabidopsis

Karrikin Improves Osmotic and Salt Stress Tolerance via the Regulation of the Redox Homeostasis in the Oil Plant Sapium sebiferum

Feature issue introduction: Light, Energy and the Environment, 2016

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