Phytoplankton growth under temperature stress: Laboratory studies using two diatoms from a tropical coastal power station site

Rajadurai, M.; Poornima, E.H.; Narasimhan, S.V.; Rao, V. N. R.; Venugopalan, V.P.

doi:10.1016/j.jtherbio.2005.01.003

Cited by 32 publications

(16 citation statements)

References 16 publications

(11 reference statements)

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“…The presence of reactive oxygen species (ROS) was detected within cultures using the fluorescent probe 2,7-dichlorodihydrofluorescein-diacetate (CM-H2DCFDA; Molecular Probes), which binds to ROS and other peroxides (Rastogi et al, 2010). The reagent was thawed at room temperature for 10 min and activated using 86.5 µL of DMSO, with 5 µL of activated reagent added to each sample (final concentration 5 µM).…”

Section: Reactive Oxygen Species Measurementsmentioning

confidence: 99%

“…The aims of this study were to investigate how an acute increase in temperature (+12 • C), comparable to those associated with MHW events and leading to thermal stress in A. minutum could alter the physiological state and biogenic sulfur cycling dynamics of A. minutum and determine how these changes might influence the composition of the Alexandrium microbiome. We hypothesised that an abrupt increase in temperature would lead to physiological impairment (Falk et al, 1996;Robison and Warner, 2006;Iglesias-Prieto et al, 1992;Rajadurai et al, 2005) and oxidative stress (Lesser, 2006) in A. minutum, leading to an upregulation of DMSP, DMS and DMSO production (McLenon and DiTullio, 2012;Sunda et al, 2002) in this high DMSP producer, which could ultimately lead to a shift in the composition of the A. minutum microbiome.…”

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confidence: 99%

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Shifts in dimethylated sulfur concentrations and microbiome composition in the red-tide causing dinoflagellate <i>Alexandrium minutum</i> during a simulated marine heatwave

et al. 2019

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Abstract. The biogenic sulfur compounds dimethyl sulfide (DMS), dimethyl sulfoniopropionate (DMSP) and dimethyl sulfoxide (DMSO) are produced and transformed by diverse populations of marine microorganisms and have substantial physiological, ecological and biogeochemical importance spanning organism to global scales. Understanding the production and transformation dynamics of these compounds under shifting environmental conditions is important for predicting their roles in a changing ocean. Here, we report the physiological and biochemical response of a robust strain of Alexandrium minutum, a dinoflagellate with the highest reported intracellular DMSP content, exposed to a 6 d increase in temperature mimicking mild and extreme coastal marine heatwave conditions (+4 and +12 ∘C). Under mild temperature increases (+4 ∘C), A. minutum growth was enhanced, with no measurable physiological stress response. However, under a very acute increase in temperature (+12 ∘C) triggering thermal stress, A. minutum growth declined, photosynthetic efficiency (FV∕FM) was impaired, and enhanced oxidative stress was observed. These physiological responses indicative of thermal stress were accompanied by increased DMS and DMSO concentrations followed by decreased DMSP concentration. At this temperature extreme, we observed a cascading stress response in A. minutum, which was initiated 6 h after the start of the experiment by a spike in DMS and DMSO concentrations and a rapid decrease in FV∕FM. This was followed by an increase in reactive oxygen species (ROS) and an abrupt decline in DMS and DMSO on day 2 of the experiment. A subsequent decrease in DMSP coupled with a decline in the growth rate of both A. minutum and its associated total bacterial assemblage coincided with a shift in the composition of the A. minutum microbiome. Specifically, an increase in the relative abundance of the operational taxonomic units (OTUs) matching Oceanicaulis (17.0 %), Phycisphaeraceae SM1A02 (8.8 %) and Balneola (4.9 %) as well as a decreased relative abundance of Maribacter (24.4 %), Marinoscillum (4.7 %) and Seohaeicola (2.7 %) were primarily responsible for differences in microbiome structure observed between temperature treatments. These shifts in microbiome structure are likely to have been driven by either the temperature itself, the changing physiological state of A. minutum cells, shifts in biogenic sulfur concentrations, the presence of other solutes, or a combination of all. Nevertheless, we suggest that these results point to the significant effect of extreme heatwaves on the physiology, growth and microbiome composition of the red-tide causing dinoflagellate A. minutum, as well as potential implications for biogenic sulfur cycling processes and marine DMS emissions.

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Section: Reactive Oxygen Species Measurementsmentioning

confidence: 99%

mentioning

confidence: 99%

Shifts in dimethylated sulfur concentrations and microbiome composition in the red-tide causing dinoflagellate <i>Alexandrium minutum</i> during a simulated marine heatwave

et al. 2019

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“…Temperature had no significant effect on the growth rate and abundance of C. calcitrans. Different species of Chaetoceros are reported to tolerate temperature ranging from 25°C to 40°C with the best at 30°C (Renaud et al, 2002;de Castro Araújo and Garcia, 2005;Rajadurai et al, 2005). In the present study, irrespective pCO 2 levels the optimum temperature for the growth of C. calcitrans was found to be 30°C, based on the high lipid content as diatoms are reported to contain maximum lipid content at optimum range (Renaud et al, 1995).…”

Section: Discussionmentioning

confidence: 45%

Interactive effect of elevated pCO2 and temperature on the larval development of an inter-tidal organism, Balanus amphitrite Darwin (Cirripedia: Thoracica)

Baragi

Anil

2015

Journal of Experimental Marine Biology and Ecology

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“…The potential impact of thermal discharge from a coastal power plant cooling system on marine organisms and their ecosystem has been studied extensively in the last decades, e.g., bacteria (Choi et al 2002;Shiah et al 2006), phytoplankton (Martínez-Arroyo et al 2000;Poornima et al 2006;Chuang et al 2009), zooplankton (Yang et al 2002;Jiang et al 2009), crab (Suresh et al 1995), fish (Teixeira et al 2009), and sedentary organisms (Lardicci et al 1999;Teixeira et al 2009). Most recent impact studies on phytoplankton focused mainly on the effects of elevated seawater temperature and residual chlorine on the biomass and productivity Rajadurai et al 2005;Chuang et al 2009). Li et al (2011) reported that the nuclear power plant thermal discharge in the subtropical Daya Bay in China strongly influenced the phytoplankton community.…”

Section: Introductionmentioning

confidence: 99%

Phytoplankton Communities Impacted by Thermal Effluents Off Two Coastal Nuclear Power Plants in Subtropical Areas of Northern Taiwan

Lo¹,

Hsu²,

Fang³

et al. 2016

Terr. Atmos. Ocean. Sci.

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This study investigates the seasonal and spatial differences in phytoplankton communities in the coastal waters off two nuclear power plants in northern Taiwan in 2009. We identified 144 phytoplankton taxa in our samples, including 127 diatoms, 16 dinoflagellates, and one cyanobacteria. Four diatoms, namely, Thalassionema nitzschioides (T. nitzschioides), Pseudo-nitzschia delicatissima (P. delicatissima), Paralia sulcate (P. sulcata), and Chaetoceros curvisetus (C. curvisetus) and one dinoflagellate Prorocentrum micans (P. micans) were predominant during the study period. Clear seasonal and spatial differences in phytoplankton abundance and species composition were evident in the study area. Generally, a higher mean phytoplankton abundance was observed in the waters off the First Nuclear Power Plant (NPP I) than at the Second Nuclear Power Plant (NPP II). The phytoplankton abundance was usually high in the coastal waters during warm periods and in the offshore waters in winter. The phytoplankton species composition was different in the NPP intake and outlet. An abundant phytoplankton increase in the outlet was frequently detected, probably due to the difference in phytoplankton species suitability to thermal stress. Our results suggest that thermal discharge may affect the phytoplankton communities by altering its species composition within a restricted area close to the NPP. This study provides basic knowledge on phytoplankton distribution patterns, essential information required to further understand the ecological impact caused by thermal effluents.

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Phytoplankton growth under temperature stress: Laboratory studies using two diatoms from a tropical coastal power station site

Cited by 32 publications

References 16 publications

Shifts in dimethylated sulfur concentrations and microbiome composition in the red-tide causing dinoflagellate <i>Alexandrium minutum</i> during a simulated marine heatwave

Shifts in dimethylated sulfur concentrations and microbiome composition in the red-tide causing dinoflagellate <i>Alexandrium minutum</i> during a simulated marine heatwave

Interactive effect of elevated pCO2 and temperature on the larval development of an inter-tidal organism, Balanus amphitrite Darwin (Cirripedia: Thoracica)

Phytoplankton Communities Impacted by Thermal Effluents Off Two Coastal Nuclear Power Plants in Subtropical Areas of Northern Taiwan

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Phytoplankton growth under temperature stress: Laboratory studies using two diatoms from a tropical coastal power station site

Cited by 32 publications

References 16 publications

Shifts in dimethylated sulfur concentrations and microbiome composition in the red-tide causing dinoflagellate &lt;i&gt;Alexandrium minutum&lt;/i&gt; during a simulated marine heatwave

Shifts in dimethylated sulfur concentrations and microbiome composition in the red-tide causing dinoflagellate &lt;i&gt;Alexandrium minutum&lt;/i&gt; during a simulated marine heatwave

Interactive effect of elevated pCO2 and temperature on the larval development of an inter-tidal organism, Balanus amphitrite Darwin (Cirripedia: Thoracica)

Phytoplankton Communities Impacted by Thermal Effluents Off Two Coastal Nuclear Power Plants in Subtropical Areas of Northern Taiwan

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Shifts in dimethylated sulfur concentrations and microbiome composition in the red-tide causing dinoflagellate <i>Alexandrium minutum</i> during a simulated marine heatwave

Shifts in dimethylated sulfur concentrations and microbiome composition in the red-tide causing dinoflagellate <i>Alexandrium minutum</i> during a simulated marine heatwave