Phytoplankton and bacterial uptake of inorganic and organic nitrogen during an induced bloom of Phaeocystis pouchetii

Sanderson, Marta P.; Bronk, Deborah A.; Nejstgaard, Jens C.; Verity, Peter G.; Sazhin, Andrey F.; Frischer, Marc E.

doi:10.3354/ame01178

Cited by 21 publications

(19 citation statements)

References 57 publications

(66 reference statements)

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“…A similar study was conducted at the same location in 2003 (Nejstgaard et al 2006, Sanderson et al 2008.…”

Section: Discussionmentioning

confidence: 92%

“…A mesocosm study similar to that presented here was conducted in 2003 and included uptake rate measurements for 2 DON substrates (urea and dissolved free amino acids [DFAA]). After depleting amended NO 3 -stocks, the Phaeocystis-dominated assemblage relied on urea for the majority (up to 80%) of its N demand as ambient concentrations of this reduced form increased (Sanderson et al 2008).…”

Section: Introductionmentioning

confidence: 99%

“…The goals of this study were to (1) induce a bloom of Phaeocystis pouchetii by addition of NO 3 -and PO 4 3 -, (2) compare uptake of 15 N-labeled DIN and DON substrates by phytoplankton and bacteria prior to and during the bloom, (3) examine dissolved nutrient availability and N uptake results with respect to phytoplankton community composition, and (4) compare traditional filtration with FCM sorting as a means of accurately quantifying phytoplankton N uptake rates. These results are compared with those from a study conducted in early spring 2003 under different initial nutrient conditions and plankton community structure, and without the use of FCM sorting to measure autotrophic N uptake (Sanderson et al 2008). ) when measured using a LI-COR 190 quantum sensor and a LI-COR 1400 data logger.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Nitrogen uptake by phytoplankton and bacteria during an induced Phaeocystis pouchetii bloom, measured using size fractionation and flow cytometric sorting

Bradley¹,

Sanderson²,

Nejstgaard³

et al. 2010

Aquat. Microb. Ecol.

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“…A similar study was conducted at the same location in 2003 (Nejstgaard et al 2006, Sanderson et al 2008.…”

Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Nitrogen uptake by phytoplankton and bacteria during an induced Phaeocystis pouchetii bloom, measured using size fractionation and flow cytometric sorting

Bradley¹,

Sanderson²,

Nejstgaard³

et al. 2010

Aquat. Microb. Ecol.

Self Cite

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“…However, this study shows that urea uptake rates can be highest relative to other N sources during the spring bloom, up to 44% of the total N uptake. There are indications that this may also be the case during spring in the Canadian Arctic (Simpson et al 2013) and during an induced Phaeocystis bloom in a Norwegian fjord (Sanderson et al 2008). The recent identification of a urea cycle in diatoms may be relevant in this regard (e.g., Armbrust et al 2004;Allen et al 2011); whereas the urea cycle in mammals serves to remove excess N, in diatoms it appears to also play an important role in the fixation of C and N and the rapid metabolic response following short-term nutrient withdrawal or introduction.…”

Section: Seasonal Phytoplankton Distributionmentioning

confidence: 97%

Nitrogen dynamics and phytoplankton community structure: the role of organic nutrients

et al. 2017

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Dissolved organic nitrogen (DON) is recognised as an important N source for phytoplankton. However, its relative importance for phytoplankton nutrition and community composition has not been studied comprehensively. This study, conducted in a typical Scottish fjord, representative of nearpristine coastal environments, evaluates the utilisation of DON and dissolved inorganic nitrogen (DIN) by different microbial size fractions and the relationship of phytoplankton community composition with DON and other parameters. The study demonstrated that DON was important in supporting phytoplankton throughout the yearly production cycle. The higherthan-expected urea uptake rates and large fraction of the spring bloom production supported by DON suggested that organic N not only contributes to regenerated production and to the nutrition of the small phytoplankton fraction, but can also contribute substantially to new production of the larger phytoplankton in coastal waters. Multivariate statistical techniques revealed two phytoplankton assemblages with peaks in abundance at different times of the year: a spring group dominated by Skeletonema spp., Thalassiosira spp., and Pseudo-nitzschia spp. group delicatissima; and a summer/autumn group dominated by Chaetoceros spp., Scrippsiella spp., and Pseudonitzschia spp. group seriata. The multivariate pattern in community composition and abundance of these taxa was significantly correlated with the multivariate pattern of DON, urea, dissolved free amino acids, DIN, temperature, salinity, and daylength, with daylength and urea being particularly important, suggesting both physical and chemical controls on community composition.

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“…For example, the supply of urea from anthropogenic sources has been linked to increased occurrences of HABs (e.g. Berg et al 1997, Sanderson et al 2008. Learning exactly how HAB species are able to take advantage of the urea-N supply to outgrow their competitors is essential in managing HABs.…”

Section: Importance Of Studying Urea Metabolismmentioning

confidence: 99%

Role of urea in microbial metabolism in aquatic systems: a biochemical and molecular review

Solomon

Collier²,

Berg³

et al. 2010

Aquat. Microb. Ecol.

234

238

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Urea synthesized commercially and formed naturally as a by-product of cellular metabolism is an important source of nitrogen (N) for primary producers in aquatic ecosystems. Although urea is usually present at ambient concentrations below 1 µM-N, it can contribute 50% or more of the total N used by planktonic communities. Urea may be produced intracellularly via purine catabolism and/or the urea cycle. In many bacteria and eukaryotes, urea in the cell can be broken down by urease into NH 4 + and CO 2 . In addition, some bacteria and eukaryotes use urea amidolyase (UALase) to decompose urea. The regulation of urea uptake appears to differ from the regulation of urease activity, and newly available genomic sequence data reveal that urea transporters in eukaryotic phytoplankton are distinct from those present in Cyanobacteria and heterotrophic bacteria with different energy sources and possibly different enzyme kinetics. The diverse metabolic pathways of urea transport, production, and decomposition may contribute to differences in the role that urea plays in the physiology and ecology of different species, and in the role that each species plays in the biogeochemistry of urea. This review summarizes what is known about urea sources and availability, use of urea as an organic N growth source, rates of urea uptake, enzymes involved in urea metabolism (i.e. urea transporters, urease, UALase), and the biochemical and molecular regulation of urea transport and metabolic enzymes, with an emphasis on the potential for genomic sequence data to continue to provide important new insights.

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Phytoplankton and bacterial uptake of inorganic and organic nitrogen during an induced bloom of Phaeocystis pouchetii

Cited by 21 publications

References 57 publications

Nitrogen uptake by phytoplankton and bacteria during an induced Phaeocystis pouchetii bloom, measured using size fractionation and flow cytometric sorting

Nitrogen uptake by phytoplankton and bacteria during an induced Phaeocystis pouchetii bloom, measured using size fractionation and flow cytometric sorting

Nitrogen dynamics and phytoplankton community structure: the role of organic nutrients

Role of urea in microbial metabolism in aquatic systems: a biochemical and molecular review

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