Phytophthora nicotianae transformants lacking dynein light chain 1 produce non-flagellate zoospores

Narayan, Reena D.; Blackman, Leila M.; Shan, Weixing; Hardham, Adrienne R.

doi:10.1016/j.fgb.2010.04.008

Cited by 24 publications

(27 citation statements)

References 72 publications

(84 reference statements)

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“…Furthermore, a single residue alteration within the terminal LRR of the human orthologue DNAL1 has been demonstrated to cause PCD (15), and lack of a LC1/DNAL1 orthologue has also been reported to yield non-flagellate zoospores in the pathogenic fungus Phytophthora nicotianae (30).…”

Section: Discussionmentioning

confidence: 99%

Functional Architecture of the Outer Arm Dynein Conformational Switch

King

Patel‐King

2012

Journal of Biological Chemistry

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Background: Ciliary dyneins monitor and respond to the mechanical state or curvature of the microtubular axoneme. Results: NMR chemical shift mapping and binding assays define functional subdomains within a key component (LC1) of this system. Conclusion: LC1 provides a direct tether between a dynein motor unit and the microtubule that modulates motor function. Significance: This study provides insight into the mechanism by which dyneins are coordinated during ciliary beating.

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Section: Discussionmentioning

confidence: 99%

Functional Architecture of the Outer Arm Dynein Conformational Switch

King

Patel‐King

2012

Journal of Biological Chemistry

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“…Transgene-mediated internuclear gene silencing was demonstrated in P. infestans [25] transformants introduced with either sense or antisense gene constructs. Stable transgenic expression of dsRNA sequences has successfully silenced target genes in P. infestans [20], [26], [29] and P. parasitica [21]. Evidence for accumulation of siRNAs was also obtained in the silenced transformants of P. infestans [20].…”

Section: Introductionmentioning

confidence: 92%

“…A key step of RNA silencing is the efficient processing of dsRNAs into short RNA duplexes of 21 to 28 nucleotides in length by a series of proteins associated with post-transcriptional gene silencing (PTGS), followed by the guided cleavage or translational repression of complementary mRNAs by the generated siRNA duplexes [15]. RNA silencing technology has become a useful experimental tool to study gene function in mammals [16], plants [17], nematodes [18], fungi [19] and oomycetes [20], [21].…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, successful development [27] and application of transient gene silencing by exogenous introduction of dsRNAs into the P. infestans protoplasts [30], [31], [32] showed that Phytophthora could process exogenous dsRNAs and initiate a silencing effect. Gene silencing has been achieved in P. parasitica [21], [28], including the use of transgenic expression of dsRNAs [21].…”

Section: Introductionmentioning

confidence: 99%

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Production of dsRNA Sequences in the Host Plant Is Not Sufficient to Initiate Gene Silencing in the Colonizing Oomycete Pathogen Phytophthora parasitica

Zhang

Wang

et al. 2011

PLoS ONE

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Species of the oomycete genus Phytophthora are destructive pathogens, causing extensive losses in agricultural crops and natural ecosystems. A potential disease control approach is the application of RNA silencing technology which has proven to be effective in improving plant resistance against a wide range of pests including parasitic plants, nematodes, insects and fungi. In this study, we tested the potential application of RNA silencing in improving plant disease resistance against oomycete pathogens. The endogenous P. parasitica gene PnPMA1 and the reporter gene GFP were used to evaluate the potential application of host induced gene silencing (HIGS). The GFP-expressing P. parasitica efficiently colonized Arabidopsis thaliana lines stably expressing GFP dsRNA and showed no obvious decrease in GFP signal intensity. Quantitative RT-PCR analyses showed no significant reductions in the abundance of GFP and PnPMA1 transcripts in P. parasitica during colonization of A. thaliana lines stably expressing GFP and PnPMA1 dsRNAs, respectively. Neither GFP siRNAs nor PnPMA1 siRNAs produced by transgenic plants were detected in P. parasitica re-isolated from infected tissues by Northern blot analyses. Phenotypic characterization of zoospores released from infected plant roots expressing PnPMA1 dsRNA showed no motility changes compared with those from wild-type plants. Similar results were obtained by analysis of zoospores released from sporulating hyphae of P. parasitica re-isolated from PnPMA1 dsRNA-expressing plant roots. Thus, the ectopic expression of dsRNA sequences in the host plant is not sufficient to initiate silencing of homologous genes in the colonizing oomycete pathogen, and this may be due to a number of different reasons including the absence of genetic machinery required for uptake of silencing signals in particular dsRNAs which are essential for environmental RNA silencing.

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“…For example, the suppressed expression of cellulose-binding elicitor lectin (CBEL) in transgenic strains of P. parasitica caused severe impartation in adhesion of the pathogen to the cellophane membrane, differentiation of lobed structures in contact with cellophane, and formation of branched aggregating hyphae (Gaulin et al 2002). The transformants silenced with PnDLCl in P. parasitica (Narayan et al 2010) released nonflagellate, nonmotile zoospores from their sporangia. High level (more than 80% reduction) of PnPMA1 silencing in P. parasitica led to the production of nonflagellate and large aberrant zoospores, rapid transition from zoospores to cysts, and a decreased germination rate of cysts, indicating that PnPMAl plays important roles in zoospore development (Zhang et al 2012).…”

Section: Genetic Manipulationmentioning

confidence: 99%

Phytophthora parasitica: a model oomycete plant pathogen

et al. 2014

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Oomycetes are eukaryotic microorganisms morphologically similar to but phylogenetically distant from true fungi. Most species in the genus Phytophthora of oomycetes are devastating plant pathogens, causing damages to both agricultural production and natural ecosystems. Tremendous progress has been achieved in recent years in diversity, evolution and lifestyles of oomycete plant pathogens, as well as on the understanding of genetic and molecular basis of oomycete-plant interactions. Phytophthora parasitica is a soilborne pathogen with a wide range of host plants and represents most species in the genus Phytophthora. In this review, we present some recent progress of P. parasitica research by highlighting important features that make it emerge as a model species of oomycete pathogens. The emerged model pathogen will facilitate improved understanding of oomycete biology and pathology that are crucial to the development of novel disease-control strategies and improved disease-control measures.

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Phytophthora nicotianae transformants lacking dynein light chain 1 produce non-flagellate zoospores

Cited by 24 publications

References 72 publications

Functional Architecture of the Outer Arm Dynein Conformational Switch

Functional Architecture of the Outer Arm Dynein Conformational Switch

Production of dsRNA Sequences in the Host Plant Is Not Sufficient to Initiate Gene Silencing in the Colonizing Oomycete Pathogen Phytophthora parasitica

Phytophthora parasitica: a model oomycete plant pathogen

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