Phytol supplementation alters plasma concentrations of formate, amino acids, and lipid metabolites in sheep

Ding, Lanlan; Matsumura, Makoto; Obitsu, Taketo; Sugino, Toshihisa

doi:10.1016/j.animal.2021.100174

Cited by 8 publications

(7 citation statements)

References 22 publications

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“…The leukocyte pellets were washed with distilled water three times and resuspended in Sepasol-RNA I Super G to extract total RNA. Free amino acids in plasma were analyzed using an automatic amino acid analyzer (JLC 500; JEOL, Tokyo, Japan) as reported [ 25 ].…”

Section: Methodsmentioning

confidence: 99%

Valine Treatment Enhances Antimicrobial Component Production in Mammary Epithelial Cells and the Milk of Lactating Goats Without Influencing the Tight Junction Barrier

Tsugami

Nii

Isobe

2023

J Mammary Gland Biol Neoplasia

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The production of antimicrobial components and the formation of less-permeable tight junctions (TJs) are important in the defense system of lactating mammary glands and for safe dairy production. Valine is a branched-chain amino acid that is actively consumed in the mammary glands and promotes the production of major milk components like β-casein; additionally, branched-chain amino acids stimulate antimicrobial component production in the intestines. Therefore, we hypothesized that valine strengthens the mammary gland defense system without influencing milk production. We investigated the effects of valine in vitro using cultured mammary epithelial cells (MECs) and in vivo using the mammary glands of lactating Tokara goats. Valine treatment at 4 mM increased the secretion of S100A7 and lactoferrin as well as the intracellular concentration of β-defensin 1 and cathelicidin 7 in cultured MECs. In addition, an intravenous injection of valine increased S100A7 levels in the milk of Tokara goats without influencing milk yield and milk components (i.e., fat, protein, lactose, and solids). In contrast, valine treatment did not affect TJ barrier function either in vitro or in vivo. These findings indicate that valine enhances antimicrobial component production without influencing milk production and TJ barrier function in lactating mammary glands; thus, valine contributes to safe dairy production.

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Section: Methodsmentioning

confidence: 99%

Valine Treatment Enhances Antimicrobial Component Production in Mammary Epithelial Cells and the Milk of Lactating Goats Without Influencing the Tight Junction Barrier

Tsugami

Nii

Isobe

2023

J Mammary Gland Biol Neoplasia

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“…More interestingly, the elevated plasma methanol concentration was followed by a subsequent increase in the plasma concentration of formate (4 h after feeding). Plasma formate can be used as a methyl group donor for the folate cycle (Washburn et al, 2015), and its concentration varies with the dietary conditions (Ding et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Plasma-free amino acids were analyzed using an automatic amino acid analyzer (JLC 500; JEOL, Tokyo, Japan). The ruminal and plasma concentrations of formate and acetate were determined using the method described by Ding et al (2021). The plasma concentrations of cysteine and homocysteine were measured according to the method described by MacCoss et al (1999).…”

Section: Chemical Analysesmentioning

confidence: 99%

Effect of ensiled total mixed rations containing sake lees on digestion, nitrogen use, and plasma metabolite concentrations in sheep

Ding

Nishioka

Obitsu

et al. 2022

Animal Science Journal

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Sake lees are a byproduct of Japanese rice wine and used as ruminant feed with high protein and ethanol contents. This study aimed to explore the effects of ensiled total mixed ration (TMR) containing sake lees on rumen fermentation, digestion, nitrogen (N) use, and plasma metabolites in sheep. Four mature wethers were assigned to a 4 Â 4 Latin square design with a factorial arrangement of ensiling treatment (non-ensiled or ensiled TMR) and types of sake lees (traditional or liquefied).Although dietary ethanol consumption was higher in sheep fed ensiled TMR, ruminal and plasma concentrations of methanol and ethanol increased after feeding in all dietary treatments, which was accompanied by the increased plasma concentrations of formate. The intake and digestion of dry matter and N were lower in sheep fed ensiled TMR than in those fed non-ensiled TMR. The plasma concentrations of most amino acids decreased in sheep fed ensiled TMR. Although plasma methionine, serine, and glycine concentrations were not altered by diet, homocysteine concentration was the highest in sheep fed ensiled TMR containing traditional sake lees. The negative impacts of ensiling treatment on N digestion and amino acid utilization should be considered for formulating ensiled TMR containing sake lees.

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“…In addition, phytol in chlorophyll is a synthetic precursor of phytanic acid, which directly determines phytanic acid concentration ( Lv et al., 2017 ). Moreover, phytol has positive effects on fat metabolism, meat quality, muscle development, and regulation of animals, which further affects animal health and animal product quality ( Yang et al., 2017 ; Bobe et al., 2020 ; Ding et al., 2021 ). In general, the content of functional components in animal products is mainly associated with feed and animal digestion and absorption ( Liu et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Dynamics and correlations of chlorophyll and phytol content with silage bacterial of different growth heights Pennisetum sinese

Lv²,

Zhou³

et al. 2022

Front. Plant Sci.

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The dynamics and correlations of chlorophyll and phytol content with silage bacterial of different growth heights Pennisetum sinese were investigated. The results demonstrated that the chlorophyll and phytol content of P. sinese before and after ensiled decreased with the increase of growth height. Ensiling significantly reduced pigment content but had no significant effect on phytol. In addition, P. sinese pigment yield before and after ensiled increased with growth heights increasing, and the yield at 150 or 180 cm was obviously higher. Moreover, the higher silage quality V-Score were at 150 or 180 cm growth heights. Furthermore, the silage microbial diversity were varied by growth heights, and some specific undesirable microorganisms (Acinetobacter, Cellvibrio, Sphingobacterium, etc.) were negatively correlated with pigment and phytol content. Therefore, with comprehensive consideration of pigment, phytol yield, and silage quality, the optimum harvest growth height of P. sinese was 150 cm. Furthermore, precise reduction of particular undesirable microorganisms maybe helps to preserve pigments and phytol.

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Phytol supplementation alters plasma concentrations of formate, amino acids, and lipid metabolites in sheep

Cited by 8 publications

References 22 publications

Valine Treatment Enhances Antimicrobial Component Production in Mammary Epithelial Cells and the Milk of Lactating Goats Without Influencing the Tight Junction Barrier

Valine Treatment Enhances Antimicrobial Component Production in Mammary Epithelial Cells and the Milk of Lactating Goats Without Influencing the Tight Junction Barrier

Effect of ensiled total mixed rations containing sake lees on digestion, nitrogen use, and plasma metabolite concentrations in sheep

Dynamics and correlations of chlorophyll and phytol content with silage bacterial of different growth heights Pennisetum sinese

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