Phytol increases adipocyte number and glucose tolerance through activation of PI3K/Akt signaling pathway in mice fed high-fat and high-fructose diet

Wang, Jianbing; Hu, Xiaoquan; Ai, Wei; Zhang, Fenglin; Yang, Kelin; Wang, Lina; Zhu, Xiaotong; Gao, Ping; Shu, Gang; Jiang, Qingyan; Wang, Songbo

doi:10.1016/j.bbrc.2017.05.160

Cited by 38 publications

(34 citation statements)

References 29 publications

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“…The C. olitorius extract was significantly more effective than orlistat in increasing the number of adipocytes and alleviation of adipocyte hypertrophy. Wang et al . confirmed that phytol (acyclic diterpene) increases the number of adipocytes and prevent obesity.…”

Section: Discussionmentioning

confidence: 95%

“…The C. olitorius extract was significantly more effective than orlistat in increasing the number of adipocytes and alleviation of adipocyte hypertrophy. Wang et al [28] confirmed that phytol (acyclic diterpene) increases the number of adipocytes and prevent obesity. Moreover, many investigators suggested that a dietary monoterpene phenolic compound of thymol and monoterpene of limonene plays a role in the browning of white adipocytes, augmentation of lipolysis, fat oxidation, and thermogenesis.…”

Section: Discussionmentioning

confidence: 98%

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Amelioration of experimental metabolic syndrome induced in rats by orlistat and Corchorus olitorius leaf extract; role of adipo/cytokines

Gomaa

Elsers

Al-Zokeim

et al. 2018

Journal of Pharmacy and Pharmacology

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Objectives To determine the efficacy of Corchorus olitorius (C. olitorius) leaf extract in the prevention of metabolic syndrome induced in rats by high‐fat diet (HFD) and compare it with that of orlistat. Methods Phytochemical analysis was performed. Effect of orlistat and C. olitorius extract on lipase activity and acute food intake were investigated. Body weight, biochemical parameters and histopathological examination were demonstrated. Key findings Corchorus olitorius extract inhibited the pancreatic lipase activity, but orlistat was more potent. Cumulative food intake has not changed by the tested agents. In obese rats, C. olitorius or orlistat significantly decreased weight gain and visceral white adipose tissue. They exhibited a significant reduction in serum glucose, total cholesterol, triglycerides, low density lipoprotein cholesterol, free fatty acids, IL‐1β, tumour necrosis factor‐α (TNF‐α), insulin and leptin levels of obese rat groups while high density lipoprotein cholesterol and adiponectin levels were significantly increased by them. Histopathological examination of the liver revealed that C. olitorius was more effective than orlistat in the alleviating of steatosis and adipocyte hypertrophy shown in obese control rats. Conclusions Corchorus olitorius is effective as orlistat in preventing obesity, hyperlipidaemia, steatosis and insulin resistance. These actions may be mediated by inhibiting of lipase activity, TNF‐α, IL‐1β and leptin resistance along with increasing of adiponectin.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 98%

Amelioration of experimental metabolic syndrome induced in rats by orlistat and Corchorus olitorius leaf extract; role of adipo/cytokines

Gomaa

Elsers

Al-Zokeim

et al. 2018

Journal of Pharmacy and Pharmacology

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“…Glucose uptake into the differentiated pBMSCs was measured using 2-NBDG as previously described with a Synergy 2 Multi-Mode Reader (Bio-Tek, USA) [5]. Glucose utilization of the differentiated pBMSCs were determined using glucose content assay kit (Applygen Technologies Inc, China) as previously described [24].…”

Section: Glucose Uptake and Utilization Assaymentioning

confidence: 99%

“…Lipid accumulation and TG contents in the differentiated pBMSCs were determined by Oil Red O staining and a TG assay kit (Applygen Technologies Inc, China) as previously described [5,23].…”

Section: Oil Red-o Staining and Triglyceride (Tg) Assaymentioning

confidence: 99%

“…Although the expanded fat mass can result from the increased adipocyte size (hypertrophy) and/or increased adipocyte number (hyperplasia or adipogenesis) [2], it has been demonstrated that hypertrophic adipose expansion is associated with obesity and impaired insulin sensitivity while hyperplasic adipose expansion or adipogenesis is linked to anti-obesity and improved insulin sensitivity [3][4][5]. Thus, stimulation of adipocyte hyperplasia or adipogenesis may contribute to prevention of obesity, insulin resistance and type 2 diabetes.…”

Section: Introductionmentioning

confidence: 99%

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Calcium Supplementation Enhanced Adipogenesis and Improved Glucose Homeostasis Through Activation of Camkii and PI3K/Akt Signaling Pathway in Porcine Bone Marrow Mesenchymal Stem Cells (pBMSCs) and Mice Fed High Fat Diet (HFD)

Zhang

Meng

et al. 2018

Cell Physiol Biochem

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Background/Aims: It has been implicated that calcium supplementation is involved in reducing body weight/fat and improving glucose homeostasis. However, the underlying mechanisms are still not fully understood. Here, we investigated the effects of calcium supplementation on adipogenesis and glucose homeostasis in porcine bone marrow mesenchymal stem cells (pBMSCs) and high fat diet (HFD)-fed mice and explored the involved signaling pathways. Methods: In vitro, pBMSCs were treated with 4 mM extracellular calcium ([Ca²⁺]_o) and/or 1 μM nifedipine, 0.1 μM BAPTA-AM, 1 μM KN-93, 50 nM wortmannin for 10 days. The intracellular calcium ([Ca²⁺]_i) levels were measured using Fluo 3-AM by flow cytometry. The adipogenic differentiation of pBMSCs was determined by Oil Red-O staining and triglyceride assay. The expression of marker genes involved in adipogenesis (peroxisome proliferator activated receptor γ (PPARγ) and CCAAT/enhancer binding protein α (C/EBPα)) and glucose uptake (glucose transporter 4 (GLUT4)), as well as the activation of Ca²⁺/calmodulin-dependent protein kinase II (CaMKII) and PI3K/Akt-FoxO1/AS160 signaling pathways were determined by Western blotting. Glucose uptake and utilization were examined using 2-NBDG assay and glucose content assay, respectively. In vivo, C57BL/6J male mice were fed a HFD (containing 1.2% calcium) without or with 0.6% (w/w) calcium chloride in drinking water for 13 weeks. The adipogenesis, glucose homeostasis and the involvement of CaMKII and PI3K/Akt signaling pathway were also assessed. Results: In vitro, [Ca²⁺]_o stimulated pBMSCs adipogenesis by increasing [Ca²⁺]_i level and activating CaMKII and PI3K/Akt-FoxO1 pathways. In addition, [Ca²⁺]_o promoted glucose uptake/utilization by enhancing AS160 phosphorylation, GLUT4 expression and translocation. However, the stimulating effects of [Ca²⁺]_o on pBMSCs adipogenesis and glucose uptake/utilization were abolished by L-VGCC blocker Nifedipine, [Ca²⁺]_i chelator BAPTA-AM, CaMKII inhibitor KN-93, or PI3K inhibitor Wortmannin. In vivo, calcium supplementation decreased body weight and fat content, increased adipocyte number, and improved glucose homeostasis, with elevated PPARγ and GLUT4 expression and PI3K/Akt activation in iWAT. Conclusion: calcium supplementation enhanced adipogenesis and glucose uptake in pBMSCs, which was coincident with the increased adipocyte number and improved glucose homeostasis in HFD-fed mice, and was associated with activation of CaMKII and PI3K/Akt-FoxO1/AS160 pathways. These data provided a broader understanding of the mechanisms underlying calcium-induced body weight/fat loss and glycemic control.

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Conjugated linoleic acid (CLA) reduces HFD‐induced obesity by enhancing BAT thermogenesis and iWAT browning via the CD36–AMPK pathway

Zhang,

Fu,

Wang

et al. 2024

Cell Biochemistry & Function

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The antiobesity effect of conjugated linoleic acid (CLA) has been reported. However, the underlying mechanisms have not been fully clarified. Thus, this study aimed to investigate the effects of CLA on thermogenesis of interscapular brown adipose tissue (iBAT) and browning of inguinal subcutaneous white adipose tissue (iWAT) and explore the possible signaling pathway. The in vivo results showed that CLA enhanced the O2 consumption and heat production in HFD (high‐fat diet)‐fed female mice by roughly 38%. Meanwhile, CLA increased the average iBAT temperature by 2°C at the room temperature and cold exposure, respectively. Correspondingly, CLA caused 1.6‐ and 2.4‐fold increases in the expression of UCP1 (uncoupling protein 1) of BAT and iWAT, respectively, suggesting the activated iBAT thermogenesis and iWAT browning in HFD‐fed female mice. Meanwhile, CLA could promote the formation of brown and beige adipocytes in differentiated stromal vascular cells (SVCs) isolated from iBAT and iWAT (the expressions of UCP1 were promoted by about twofold changes). In possible mechanisms, CLA stimulated the expression of CD36 and the activation of the AMPK pathway in mice iBAT and iWAT as well as the differentiated SVCs. However, inhibition of CD36 and AMPK (adenosine 5′‐monophosphate‐activated protein kinase) abolished the promotive effects of CLA on brown and beige adipocytes formation. Hence, we showed that CLA reduced HFD‐induced obesity through enhancing iBAT thermogenesis and iWAT browning via the CD36–AMPK pathway.

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Phytol increases adipocyte number and glucose tolerance through activation of PI3K/Akt signaling pathway in mice fed high-fat and high-fructose diet

Cited by 38 publications

References 29 publications

Amelioration of experimental metabolic syndrome induced in rats by orlistat and Corchorus olitorius leaf extract; role of adipo/cytokines

Amelioration of experimental metabolic syndrome induced in rats by orlistat and Corchorus olitorius leaf extract; role of adipo/cytokines

Calcium Supplementation Enhanced Adipogenesis and Improved Glucose Homeostasis Through Activation of Camkii and PI3K/Akt Signaling Pathway in Porcine Bone Marrow Mesenchymal Stem Cells (pBMSCs) and Mice Fed High Fat Diet (HFD)

Conjugated linoleic acid (CLA) reduces HFD‐induced obesity by enhancing BAT thermogenesis and iWAT browning via the CD36–AMPK pathway

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