Phytohormones regulate convergent and divergent responses between individual and combined drought and pathogen infection

Gupta, Aarti; Sinha, Ranjita; Fernandes, Joel Lars; Abdelrahman, Mostafa; Burritt, David J.; Tran, Lam-Son Phan

doi:10.1080/07388551.2019.1710459

Cited by 42 publications

(32 citation statements)

References 140 publications

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“…Important roles of phytohormones in regulating plant growth and development, and their responses to resist abiotic and biotic stresses have been published by numerous studies [ 4 , 20 , 56 , 57 ]. According to the results of MapMan analysis, 108 DEGs including 92 up-regulated and 16 down-regulated genes were shown to be involved in various hormone-related metabolic and signaling pathways ( Supplementary Table S7 ).…”

Section: Resultsmentioning

confidence: 99%

“…Important roles of phytohormones in regulating plant growth and development, and their responses to resist abiotic and biotic stresses have been published by numerous studies [ 4 , 20 , 56 , 57 ]. Notably, in the ABA biosynthetic pathway, two genes OvNCED3 (unigene0064567) and OvNCED4 (unigene0008736) belonging to the nine-cis-epoxycarotenoid dioxygenase ( NCED ) family, were both significantly up-regulated in TD/MH comparison with log 2 (FC) values of 3.40 and 3.10, respectively ( Supplementary Table S7 ), as also verified by RT-qPCR ( Figure 11 D).…”

Section: Discussionmentioning

confidence: 99%

“…As sessile organisms, plants are continuously exposed to a plethora of environmental stresses, including abiotic and biotic stresses, which severely constrain their growth and productivity. The major abiotic stresses are salinity, drought, heat, cold, chilling, flooding, ultraviolet (UV) radiation, and heavy metal toxicity [ 1 , 2 , 3 , 4 , 5 ]. Abiotic stress is the prominent threat in natural conditions under continuous climate change, and has caused more than 50% of crop losses worldwide [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome Analysis Reveals Potential Roles of Abscisic Acid and Polyphenols in Adaptation of Onobrychis viciifolia to Extreme Environmental Conditions in the Qinghai-Tibetan Plateau

et al. 2020

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A detailed understanding of the molecular mechanisms of plant stress resistance in the face of ever-changing environmental stimuli will be helpful for promoting the growth and production of crop and forage plants. Investigations of plant responses to various single abiotic or biotic factors, or combined stresses, have been extensively reported. However, the molecular mechanisms of plants in responses to environmental stresses under natural conditions are not clearly understood. In this study, we carried out a transcriptome analysis using RNA-sequencing to decipher the underlying molecular mechanisms of Onobrychis viciifolia responding and adapting to the extreme natural environment in the Qinghai-Tibetan Plateau (QTP). The transcriptome data of plant samples collected from two different altitudes revealed a total of 8212 differentially expressed genes (DEGs), including 5387 up-regulated and 2825 down-regulated genes. Detailed analysis of the identified DEGs uncovered that up-regulation of genes potentially leading to changes in hormone homeostasis and signaling, particularly abscisic acid-related ones, and enhanced biosynthesis of polyphenols play vital roles in the adaptive processes of O. viciifolia. Interestingly, several DEGs encoding uridine diphosphate glycosyltransferases, which putatively regulate phytohormone homeostasis to resist environmental stresses, were also discovered. Furthermore, numerous DEGs encoding transcriptional factors, such as members of the myeloblastosis (MYB), homeodomain-leucine zipper (HD-ZIP), WRKY, and nam-ataf1,2-cuc2 (NAC) families, might be involved in the adaptive responses of O. viciifolia to the extreme natural environmental conditions. The DEGs identified in this study represent candidate targets for improving environmental stress resistance of O. viciifolia grown in higher altitudes of the QTP, and can provide deep insights into the molecular mechanisms underlying the responses of this plant species to the extreme natural environmental conditions of the QTP.

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Section: Resultsmentioning

confidence: 99%

“…Important roles of phytohormones in regulating plant growth and development, and their responses to resist abiotic and biotic stresses have been published by numerous studies [ 4 , 20 , 56 , 57 ]. Notably, in the ABA biosynthetic pathway, two genes OvNCED3 (unigene0064567) and OvNCED4 (unigene0008736) belonging to the nine-cis-epoxycarotenoid dioxygenase ( NCED ) family, were both significantly up-regulated in TD/MH comparison with log 2 (FC) values of 3.40 and 3.10, respectively ( Supplementary Table S7 ), as also verified by RT-qPCR ( Figure 11 D).…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transcriptome Analysis Reveals Potential Roles of Abscisic Acid and Polyphenols in Adaptation of Onobrychis viciifolia to Extreme Environmental Conditions in the Qinghai-Tibetan Plateau

et al. 2020

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“…The parameters SODR, SODS, APXR, APXS, CATR, and CATS grouped together and were more affected by the treatment CW1 + MP. Gupta [63] reported that different phytohormones showed complicated convergent and divergent responses to combat single and multiple stresses in plants. Overall, PGPB-mediated systemic resistance in soybean is the result of combined effect of phytohormones, antioxidants, and osmolytes that decrease the disease incidence and improve the resistance and growth of plants.…”

Section: Discussionmentioning

confidence: 99%

Identification of New Biocontrol Agent against Charcoal Rot Disease Caused by Macrophomina phaseolina in Soybean (Glycine max L.)

et al. 2020

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Controlling agricultural pests using suitable biocontrol agents has been considered the best strategy for sustainable agriculture. Charcoal rot caused by a necrotrophic fungus Macrophomina phaseolina is responsible for a 30–50% annual reduction in soybean yield worldwide. Little is known about the role of Bacillus clausii in reducing charcoal rot disease severity in the soybean crop. In this study, we investigated plant growth promoting and antagonistic potential of Pseudomonas putida (MT604992) and Bacillus clausii (MT604989) against charcoal rot disease incidence in soybean. Among twenty bacteria isolated from soil and water samples of two different hot springs of Gilgit-Baltistan, Pakistan, 80% were siderophore positive; 65% were hydrogen cyanide (HCN) positive; 55%, 30%, and 75% were phosphate, potassium, and zinc solubilizers, respectively. Based on higher antagonistic activities and plant growth promoting traits five strains were selected for in vitro screening. Out of all tested strains, Pseudomonas putida and Bacillus clausii showed a significant increase in germination, growth, and disease suppression in soybean. These strains produced a pronounced increase in relative water content, photosynthetic pigments, membrane stability, proline, antioxidant enzymes status, phytohormones content (Salicylic acid, and Jasmonic acid), and disease suppression in comparison to control plants. Bacillus clausii mitigated the disease by 97% with a marked increase in the proline content (73% and 89%), superoxide dismutase (356% and 208%), peroxidase (439% and 138.6%), catalase (255.8% and 80.8%), and ascorbate peroxidase (228% and 90%) activities in shoots and roots, respectively. Infected plants showed an increase in salicylic acid and jasmonic acid content which was further increased with the application of the selected strains to increase resistance against pathogens. To our knowledge, this is the first study showing a rise in salicylic acid and jasmonic acid in Macrophomina phaseolina infected plants. These two strains are suggested as a cost-effective, eco-friendly, and sustainable alternative to chemical fungicides. However, there is a need to explore the field testing and molecular mechanisms leading to disease suppression by these strains.

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“…According to our findings, both lines of transgenic Arabidopsis were hypersensitive to the presence of exogenous ABA, illustrated by substantially lower rates of germination and seedlings with normal cotyledon development as well as reduced root growth ( Figure 5A-C). Ever since its discovery, ABA has been intensively studied and its functions have been linked to various stress responses, from water relation such as drought, cold or heat to wounding or pathogen infection, by inducing stomatal closure or regulating the expression of ABA-responsive genes [17,[79][80][81]. Thus, such ABA-hypersensitive traits imply ABA-dependent activities of GmRR34 and possibly faster responses to osmotic changes in the transgenic plants.…”

Section: Discussionmentioning

confidence: 99%

Heterologous Expression of a Soybean Gene RR34 Conferred Improved Drought Resistance of Transgenic Arabidopsis

Nghia

Chuong

Hoang

et al. 2020

Plants

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Two-component systems (TCSs) have been identified as participants in mediating plant response to water deficit. Nevertheless, insights of their contribution to plant drought responses and associated regulatory mechanisms remain limited. Herein, a soybean response regulator (RR) gene RR34, which is the potential drought-responsive downstream member of a TCS, was ectopically expressed in the model plant Arabidopsis for the analysis of its biological roles in drought stress response. Results from the survival test revealed outstanding recovery ratios of 52%–53% in the examined transgenic lines compared with 28% of the wild-type plants. Additionally, remarkedly lower water loss rates in detached leaves as well as enhanced antioxidant enzyme activities of catalase and superoxide dismutase were observed in the transgenic group. Further transcriptional analysis of a subset of drought-responsive genes demonstrated higher expression in GmRR34-transgenic plants upon exposure to drought, including abscisic acid (ABA)-related genes NCED3, OST1, ABI5, and RAB18. These ectopic expression lines also displayed hypersensitivity to ABA treatment at germination and post-germination stages. Collectively, these findings indicated the ABA-associated mode of action of GmRR34 in conferring better plant performance under the adverse drought conditions.

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Phytohormones regulate convergent and divergent responses between individual and combined drought and pathogen infection

Cited by 42 publications

References 140 publications

Transcriptome Analysis Reveals Potential Roles of Abscisic Acid and Polyphenols in Adaptation of Onobrychis viciifolia to Extreme Environmental Conditions in the Qinghai-Tibetan Plateau

Transcriptome Analysis Reveals Potential Roles of Abscisic Acid and Polyphenols in Adaptation of Onobrychis viciifolia to Extreme Environmental Conditions in the Qinghai-Tibetan Plateau

Identification of New Biocontrol Agent against Charcoal Rot Disease Caused by Macrophomina phaseolina in Soybean (Glycine max L.)

Heterologous Expression of a Soybean Gene RR34 Conferred Improved Drought Resistance of Transgenic Arabidopsis

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