Phytohormones and Elicitors Enhanced the Ecdysteroid and Glycosylflavone Content and Antioxidant Activity of Silene repens

Abstract

In the course of the ongoing chemical study of species of Silene genus, S. repens Patrin as a common species of the genus, was selected as the object of this study. Using high-performance liquid chromatography with photodiode array detection and electrospray ionization triple quadrupole mass spectrometric detection (HPLC-PDA-ESI-tQ-MS), the presence of 12 ecdysteroids and 6 glycosylflavones was established in S. repens introduced seedlings. 20-Hydroxyecdysone and polypodine B, as well as sileneside E and schaftoside, were the dominant compounds in introduced seedlings of S. repens. The effect of exogenous phytohormones and elicitors on the productivity and accumulation of ecdysteroids and glycosylflavones in introduced seedlings of S. repens was investigated for the first time. It was found that the use of ethyl arachidonate (100 mg/L) to increase the productivity of S. repens is justified. To obtain S. repens with a high content of ecdysteroids and glycosylflavones, it is recommended to apply epibrassinolide (100 mg/L) and 4-chlorophenoxyacetic acid (100 mg/L), respectively. Antioxidant activity of S. repens against 2,2-diphenyl-1-picrylhydrazyl radical (DPPH·) was determined, and it was revealed that sileneside E and schaftoside, as well as meloside A and isovitexin had the highest antioxidant activity among the studied compounds of S. repens.

Green Waste from Cucumber (Cucumis sativus L.) Cultivation as a Source of Bioactive Flavonoids with Hypolipidemic Potential

“…Only isovitexin-7-O-(6′′-O-glucosyl)-glucoside was found in all cultivars, whereas the remaining compounds were found in one to four cultivars. (34), and isovitexin-6′′-O-p-coumarate (35), have previously been identified by us in cucumber leaves [13], and were found for the first time in the cucumber waste green biomass.…”

“…Three compounds, 9, 14, and 18, yielded protonated ions with m/z 903 and, in combination with their UV patterns (λmax 270 ± 1, 290 ± 1, 319 ± 3 nm), indicated the loss of the coumarate fragment (m/z 903→757), followed by the loss of two hexose moieties (m/z 757→595, 433) (Figure 7a). (35), have previously been identified by us in cucumber leaves [13], and were found for the first time in the cucumber waste green biomass.…”

“…This approach has previously been employed for the analysis of cucumber flower flavonoids [14]. The separation of these extracts provided the identification of 38 flavonoids based on their retention times, ultraviolet and mass spectrometric spectra, and a comparison with the reference standards and literature data [9][10][11][12][13][14][33][34][35][36] (Figure 3, Table 4 and Table S4). Twenty compounds were thoroughly characterized (the first-level metabolite identification according to their structures is shown in Figure 4) [37].…”

Effect of Brassinolide and Chitosan on Growth and Chemical Composition of Aglaonema commutatum plant

“…Chitosan stimulates root growth by inducing enzymes, such as chitinases, pectinases, and glucanases, or by inducing IAA synthesis which increase new roots initiation [27]. Treatment of Silene repens plant with brassinolide led to an increase in root fresh weight [32]. Treatment with chitosan and brassinolide affected roots dry weight significantly.…”

Effect of Silica Xerogel Functionalization on Intensification of Rindera graeca Transgenic Roots Proliferation and Boosting Naphthoquinone Production

“…As we demonstrated previously [14,23], the application of MTMS-based xerogel materials (with methyl groups on the surface) can significantly enhance plant secondary metabolism with preserved growth ability. Another key aspect of the interaction between the plant biomass and applied biomaterial is the chemical structure of the latter, including the presence of specific chemical residues or substituents [29][30][31][32]. The impact of individual organic groups in the structure of experimental xerogel materials on plant biomass growth and secondary metabolite production is still not fully known.…”