Phytohemagglutinin-stimulated leukocyte-conditioned medium induces differentiation in acute promyelocytic leukemia cells in vitro

Selypes, A.; Virág, I

doi:10.1016/0145-2126(88)90023-9

Cited by 2 publications

(1 citation statement)

References 15 publications

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“…Lo u is, MO, USA), 10 -1 mol/L α-mo no ti yog li se rol (Sig ma Che mi cal Co., St. Lo u is, MO, USA), 100 µL of su per na tant from 10 ng/mL phyto he magg lu ti nin (PHA, Bi oc hrom KG, Ber lin, Ger many) sti mu la ted pe rip he ral blo od mono nuc le ar cells 28 at a fi nal vo lu me of 1.1 ml in 35 mm pet ri dis hes. Co lony growth was sti mu la ted with 3 IU/ml erit ro po i e tin (EPO; Ep rex, Ci lag AG, Zug, Swit zer land), 50 ng/ml gra nu locy te mac ropha ge-co lony sti mu la ting fac tor (GM-CSF; Le u comax, San doz, No var tis Far ma AG, Ba sel, Swe den) and 10 ng/ml gra nu locy te-co lony sti mu la ting factor (G-CSF; Ne u po gen, Am gen, F. Hoff mann-LA Roc he Ltd. Ba sel, Swe den).…”

Section: Clo No Ge Nic As Says For Com Mit Ted He Mo Po I E Tic Pro Gmentioning

confidence: 99%

Combination of Early and Late Growth Factors to Enrich Transplantable Cord Blood CD34+ Cells in Short Term Cultures

Süzergöz¹,

Kuruca²

2010

Turkiye Klinikleri J Med Sci

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A AB BS S T TR RA AC CT T O Ob bj je ec ct ti iv ve e: : Alt ho ugh um bi li cal cord blo od (UCB) is a use ful so ur ce of stem and pro geni tor cells for bo ne mar row trans plan ta ti on, it is not pos sib le to ob ta in suf fi ci ent num ber of trans plantab le cells from a sing le hu man um bi li cal cord for trans plan ta ti on in adult pa ti ents. In this study, we com bi ned early and la te growth fac tors in short term cul tu res to en rich cord blo od CD34+ he ma topo i e tic pro ge ni tor cell co unt for adult pa ti ents. M Ma a t te e r ri i a al l a an nd d M Me et t h ho od ds s: : Cord blo od mo no nuc le ar cells (CBMC) har ves ted from 15 he althy vo lun te ers, de li ve ring in term, in cu ba ted in cul tu re me di um supp le men ted with in ter le u kin-3 (IL-3), IL-6, IL-11, stem cell fac tor, flt3/flk2 li gand and throm bo po i etin for 14 days. Star ting cells and cul tu red cells (day 7 and 14 cells) we re eva lu a ted for; CD4, CD8, CD33, CD34, CD38 and HLA-DR by flow cyto metry and se e ded in se mi so lid methy lcel lu lo se cul ture for de ter mi ning BFU-E, CFU-GM and CFU-GEMM co lony for ming abi li ti es. R Re e s su ul lt ts s: : In the shortterm cul tu res, to tal CBMC co unt sig ni fi cantly in cre a sed (p= 0.0001). Com pa red to star ting cells, the ex pres si ons of CD34 (p= 0.002), CD33 (p= 0.0001), HLA-DR (p= 0.0001) and CD8 (p= 0.002) in cre ased, but tho se of CD38 (p= 0.057) and CD4 (p= 0.0001) we re sup pres sed on day 14 of cul tu res. The re we re an in cre a se in ge ne ra ti on of CFU-GM (p= 0.131) and CFU-GEMM (p= 0.134) co lo ni es and a signi fi cant dec re a se in ge ne ra ti on of BFU-E co lo ni es (p= 0.0001). C Co on nc c l lu u s si i o on n: : Ex vi vo ex pan si on of CBMC, un der com bi na ti on of the growth fac tors, con tri bu te to CD34+ pro ge ni tor cell co unt and may help to re ach suf fi ci ent amo unt of trans plan tab le cells for adult pa ti ents. Sus pen si on cul tu res may lead to eli mi na ti on of graft-ver sus-host di se a se risk and pro tec ti on of graft-ver sus-le u ke mi a ef fect, through the dec re a se of CD4+ T cells and ma in te nan ce of CD8+ T lymphocy tes, res pec ti vely.K Ke ey y W Wo or rd ds s: : Cord blo od stem cell trans plan ta ti on; an ti gens, CD34; cell cul tu re tech ni qu es Ö ÖZ ZE ET T A Am ma aç ç: : Gö bek kor don ka nı kök ve pro je ni tör hüc re açı sın dan ke mik ili ği trans plan tas yo nu için el ve riş li bir kay nak ol ma sı na kar şın tek bir in san gö bek kor do nun dan eriş kin has ta la ra na kil için ye ter li sa yı da hüc re el de et me ola na ğı bu lun ma mak ta dır. Ça lış ma mız da kor don ka nı CD34+ he mopo e tik pro je ni tör hüc re le ri eriş kin has ta la ra ye te cek dü zey de ço ğalt mak ama cıy la er ken ve geç etki li bü yü me fak tör le ri ni kı sa sü re li kül tür ler de kom bi ne et tik. G Ge e r re eç ç v ve e Y Yö ön n t te em ml le er r: : Mi a dın da do ğum ya pan 15 sağ lık lı gö nül lü den kor don ka nı mo no nük le er hüc re le ri alı na rak in ter lö kin-3 (IL-3), IL-6, IL-...

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Section: Clo No Ge Nic As Says For Com Mit Ted He Mo Po I E Tic Pro Gmentioning

confidence: 99%

Combination of Early and Late Growth Factors to Enrich Transplantable Cord Blood CD34+ Cells in Short Term Cultures

Süzergöz¹,

Kuruca²

2010

Turkiye Klinikleri J Med Sci

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The biology of hairy cell leukemia

Nielsen¹

1995

APMIS

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Phytohemagglutinin-stimulated leukocyte-conditioned medium induces differentiation in acute promyelocytic leukemia cells in vitro

Cited by 2 publications

References 15 publications

Combination of Early and Late Growth Factors to Enrich Transplantable Cord Blood CD34+ Cells in Short Term Cultures

Combination of Early and Late Growth Factors to Enrich Transplantable Cord Blood CD34+ Cells in Short Term Cultures

The biology of hairy cell leukemia

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