Phytoestrogens Enhance the Vascular Actions of the Endocannabinoid Anandamide in Mesenteric Beds of Female Rats

Peroni, Roxana N.; Abramoff, Tamara; Neuman, Isabel; Podestá, Ernesto J.; Adler‐Graschinsky, Edda

doi:10.1155/2012/647856

Cited by 9 publications

(5 citation statements)

References 29 publications

(45 reference statements)

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“…It is important to note, in contrast to our previous report that included analysis of eCB system expression in the upper small intestinal epithelium of mice maintained on WD and SD [Lab Diet 5001 used in (Argueta and DiPatrizio, 2017)], in this study we used a soy protein-free Teklad 2020x as a control SD in order to eliminate any potential effects of phytoestrogen-containing soy protein on eCB metabolism or behavior [see (McFarland et al., 2004; Thors et al., 2007, 2008; Peroni et al., 2012)]. We found two differences in results when comparing use of the two control diets versus WD.…”

Section: Resultsmentioning

confidence: 99%

Cannabinoid CB1 Receptors Inhibit Gut-Brain Satiation Signaling in Diet-Induced Obesity

et al. 2019

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Gut-brain signaling controls feeding behavior and energy homeostasis; however, the underlying molecular mechanisms and impact of diet-induced obesity (DIO) on these pathways are poorly defined. We tested the hypothesis that elevated endocannabinoid activity at cannabinoid CB 1 receptor (CB 1 Rs) in the gut of mice rendered DIO by chronic access to a high fat and sucrose diet for 60 days inhibits nutrient-induced release of satiation peptides and promotes overeating. Immunoreactivity for CB 1 Rs was present in enteroendocrine cells in the mouse’s upper small-intestinal epithelium that produce and secrete the satiation peptide, cholecystokinin (CCK), and expression of mRNA for CB 1 Rs was greater in these cells when compared to non-CCK producing cells. Oral gavage of corn oil increased levels of bioactive CCK (CCK-8) in plasma from mice fed a low fat no-sucrose diet. Pretreatment with the cannabinoid receptor agonist, WIN55,212-2, blocked this response, which was reversed by co-administration with the peripherally-restricted CB 1 R neutral antagonist, AM6545. Furthermore, monoacylglycerol metabolic enzyme function was dysregulated in the upper small-intestinal epithelium from DIO mice, which was met with increased levels of a variety of monoacylglycerols including the endocannabinoid, 2-arachidonoyl- sn -glycerol. Corn oil failed to affect levels of CCK in DIO mouse plasma; however, pretreatment with AM6545 restored the ability for corn oil to stimulate increases in levels of CCK, which suggests that elevated endocannabinoid signaling at small intestinal CB 1 Rs in DIO mice inhibits nutrient-induced CCK release. Moreover, the hypophagic effect of AM6545 in DIO mice was reversed by co-administration with the CCK A receptor antagonist, devazepide. Collectively, these results provide evidence that hyperphagia associated with DIO is driven by a mechanism that includes CB 1 R-mediated inhibition of gut-brain satiation signaling.

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Section: Resultsmentioning

confidence: 99%

Cannabinoid CB1 Receptors Inhibit Gut-Brain Satiation Signaling in Diet-Induced Obesity

et al. 2019

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“…In regard of this, the nociceptive response evoked by the stimulation of the vanilloids receptor of type 1 (TRPV1) by Anandamide, which is one of the two most abundant endocannabinoids in our body [Iannotti et al, ,], seems also mediated by an increased synthesis of CGRP [Iannotti et al, ]. In particular, it has been described that the Anandamide‐dependent activation of TRPV1 channels promotes the CGRP synthesis and release which cause the vasodilation and the immune cells infiltration in injured tissues including the mesentery [Peroni et al, ]. Interestingly, the CGRP functianality is also regulated by estrogenic like compounds.…”

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confidence: 99%

“…Interestingly, the CGRP functianality is also regulated by estrogenic like compounds. It has been showed, in fact, that isoflavonici compounds (coumponds less potent than estrogenic) are able to promote the levels of CGRP in mesentery [Peroni et al, ]. In menopausal or older women, the CGRP levels are significantly reduced.…”

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confidence: 99%

CGRP and Visceral Pain: The Role of Sex Hormones in In Vitro Experiment

et al. 2016

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A large number of studies have showed that women reported feeling pain more acutely than men. In support of this hypothesis, many research groups proved that in different animals model of pain the sex hormones regulate the somatic and visceral sensitivity to different noxious stimuli. Therefore, in this study, we went to evaluate if estrogen hormones by regulating the CGRP levels are implicated during the visceral pain transmission. Toward this aim, we have investigated the effect of 17β-estradiol in regulating the synthesis and release of CGRP, as well as the expression levels of the opioid receptor of type K. In order to gain information about the potential effects of 17β-estradiol on K-opioid receptor expression and activity, we have cultured F11 cells. Our results revealed that, when F11 cells were short-term exposed (30 min) to 17β-estradiol, the expression of the opioid K receptor was not significantly modified. We carried out enzyme immunoassay analysis to evaluate the potential effects of short-term exposure to 17-estradiol (30 min) on the release of CGRP in F11 cells. The results obtained showed that 17β-estradiol at the dose of 100 nM is able to induce the release of CGRP from F11 cells; whereas, a higher dose of 17β-estradiol (200 nM) did not produce significant effects when compared to control. In conclusion, all these findings suggest that the 17β-estradiol-regulated release of CGRP could at least in part provide a rational explanation for the difference of gender in the visceral pain sensitivity. J. Cell. Biochem. 118: 510-517, 2017. © 2016 Wiley Periodicals, Inc.

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“…The CGRP is delivered, at least in part, as an outcome of activation of the AEA-induced TRPV1 receptor. 42 There is an eCB molecule known as N-oleoylethanolamine (OEA) that was originally found mainly in the small intestine and was related to regulation of food intake and weight loss. However, recent reports in rat mesenteric arteries noted that OEA levels are approximately 100-fold greater than AEA levels.…”

Section: Active Compounds Related To Anandamide and Its Cardiovasculamentioning

confidence: 99%

“…The CGRP is delivered, at least in part, as an outcome of activation of the AEA-induced TRPV1 receptor. 42…”

Section: Active Compounds Related To Anandamide and Its Cardiovasculamentioning

confidence: 99%

Anandamide and endocannabinoid system: an attractive therapeutic approach for cardiovascular disease

Giménez

Noriega

Kassuha

et al. 2018

Therapeutic Advances in Cardiovascular Disease

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Cardiovascular disease is currently not adequately managed and has become one of the main causes of morbidity and mortality worldwide. Current therapies are inadequate in terms of preventing its progression. There are several limitations, such as poor oral bioavailability, side effects, low adherence to treatment, and high dosage frequency of formulations due to the short half-life of the active ingredients used, among others. This review aims to highlight the most relevant aspects of the relationship between the cardiovascular system and the endocannabinoid system, with special attention to the possible translational effect of the use of anandamide in cardiovascular health. The deep and detailed knowledge of this interaction, not always beneficial, and that for years has gone unnoticed, is essential for the development of new therapies. We discuss the most recent and representative results obtained in the field of basic research, referring to the aforementioned subject, emphasizing fundamentally the main role of nitric oxide, renal physiology and its deregulation in pathological processes.

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Phytoestrogens Enhance the Vascular Actions of the Endocannabinoid Anandamide in Mesenteric Beds of Female Rats

Cited by 9 publications

References 29 publications

Cannabinoid CB1 Receptors Inhibit Gut-Brain Satiation Signaling in Diet-Induced Obesity

Cannabinoid CB1 Receptors Inhibit Gut-Brain Satiation Signaling in Diet-Induced Obesity

CGRP and Visceral Pain: The Role of Sex Hormones in In Vitro Experiment

Anandamide and endocannabinoid system: an attractive therapeutic approach for cardiovascular disease

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