Phytoestrogen coumestrol: Antioxidant capacity and its loading in albumin nanoparticles

Montero, Guillermo; Arriagada, Francisco; Günther, Germán; Bollo, Soledad; Mura, Francisco; Berrios, Eduardo; Morales, Javier

doi:10.1016/j.ijpharm.2019.03.029

Cited by 34 publications

(14 citation statements)

References 72 publications

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“…Thus, the mechanism and possible treatment methods of these particular alterations remain partially unknown [6,9,10]. Coumestrol (COM) is a potent, naturally occurring phytoestrogen that belongs to the coumestans family and is widely found in soybeans, legumes, and brussels sprouts [11]. Like other phytoestrogens, coumestrol possesses estrogen-like activity due to its high affinity for estrogen receptors ERα and Erβ [12].…”

Section: Introductionmentioning

confidence: 99%

The Influence of Coumestrol on Sphingolipid Signaling Pathway and Insulin Resistance Development in Primary Rat Hepatocytes

et al. 2021

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Coumestrol is a phytoestrogen widely known for its anti-diabetic, anti-oxidant, and anti-inflammatory properties. Thus, it gets a lot of attention as a potential agent in the nutritional therapy of diseases such as obesity and type 2 diabetes. In our study, we evaluated whether coumestrol affects insulin resistance development via the sphingolipid signaling pathway in primary rat hepatocytes. The cells were isolated from the male Wistar rat's liver with the use of collagenase perfusion. Next, we incubated the cells with the presence or absence of palmitic acid and/or coumestrol. Additionally, some groups were incubated with insulin. The sphingolipid concentrations were assessed by HPLC whereas the expression of all the proteins was evaluated by Western blot. Coumestrol markedly reduced the accumulation of sphingolipids, namely, ceramide and sphinganine through noticeable inhibition of the ceramide de novo synthesis pathway in insulin-resistant hepatocytes. Moreover, coumestrol augmented the expression of fatty acid transport proteins, especially FATP5 and FAT/CD36, which also were responsible for excessive sphingolipid accumulation. Furthermore, coumestrol altered the sphingolipid salvage pathway, which was observed as the excessive deposition of the sphingosine-1-phosphate and sphingosine. Our study clearly showed that coumestrol ameliorated hepatic insulin resistance in primary rat hepatocytes. Thus, we believe that our study may contribute to the discovery of novel preventive and therapeutic methods for metabolic disorders.

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Section: Introductionmentioning

confidence: 99%

The Influence of Coumestrol on Sphingolipid Signaling Pathway and Insulin Resistance Development in Primary Rat Hepatocytes

et al. 2021

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“…It is known that the main phenolic components of extracts, such as coumestrol, formononetin and biochanin A, are effective scavengers of ROS able to modulate intracellular glutathione levels, as well as the activity of superoxide dismutase or cyclooxygenase [ 8 ]. Recent studies showed that the binding of coumestrol with bovine serum albumin preserved its antioxidant properties [ 38 ]. Previous results demonstrated that isoflavones from T. pratense , mainly formononetin and biochanin A, decreased ROS generation and hindered radical spreading via cellular-membrane stiffening, which was accompanied by the downregulation of MCF-7 cell migration [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

Cicer arietinum L. Sprouts’ Influence on Mineralization of Saos-2 and Migration of MCF-7 Cells

et al. 2020

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In the present study, we investigated the biological activity of four extracts obtained from Cicer arietinum L. sprouts. The fermentation of the sprouts with Lactobacillus casei and their incubation with β-glucosidase elevated the concentrations of isoflavonoids, especially coumestrol, formononetin and biochanin A. To study the biological activity of C. arietinum, the human osteosarcoma Saos-2 and human breast cancer MCF-7 cell lines were used. The extracts obtained from fermented sprouts exhibited the strongest ability to decrease intracellular oxidative stress in both types of cells. They augmented mineralization and alkaline phosphatase activity in Saos-2 cells, as well as diminished the secretion of interleukin-6 and tumor necrosis factor α. Simultaneously, the extracts, at the same doses, inhibited the migration of MCF-7 cells. On the other hand, elevated concentrations of C. arietinum induced apoptosis in estrogen-dependent MCF-7 cells, while lower doses stimulated cell proliferation. These results are important for carefully considering the use of fermented C. arietinum sprouts as a dietary supplement component for the prevention of osteoporosis.

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“…In addition to the classical flavonoid structure, coumestrol has a furan ring in the junction between the C and B rings (69) and one hydroxyl group each at the C4 and C7 carbons, similar to the structure of estradiol (57). These moieties confer the ability to bind mammalian estrogen receptors (70) and provide free radical scavenging properties (69,71), thus suggesting that coumestans may provide protection against breast, prostate, and ovarian cancers (72)(73)(74).…”

Section: Coumestansmentioning

confidence: 99%

Beyond Metabolism: The Complex Interplay Between Dietary Phytoestrogens, Gut Bacteria, and Cells of Nervous and Immune Systems

et al. 2020

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Phytoestrogen coumestrol: Antioxidant capacity and its loading in albumin nanoparticles

Cited by 34 publications

References 72 publications

The Influence of Coumestrol on Sphingolipid Signaling Pathway and Insulin Resistance Development in Primary Rat Hepatocytes

The Influence of Coumestrol on Sphingolipid Signaling Pathway and Insulin Resistance Development in Primary Rat Hepatocytes

Cicer arietinum L. Sprouts’ Influence on Mineralization of Saos-2 and Migration of MCF-7 Cells

Beyond Metabolism: The Complex Interplay Between Dietary Phytoestrogens, Gut Bacteria, and Cells of Nervous and Immune Systems

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