Phytodegradation of organophosphorus compounds by transgenic plants expressing a bacterial organophosphorus hydrolase

Wang, Xiaoxue; Wu, Ningfeng; Guo, Jun; Chu, Xiaoyu; Tian, Jian; Yao, Bin; Fan, Yanting

doi:10.1016/j.bbrc.2007.10.193

Cited by 52 publications

(15 citation statements)

References 27 publications

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“…It is an ecologically and economically attractive technique for remediating soils polluted with organics [8][9][10]. Plants remove pollutants through one or more biologically active processes, such as accumulation, transformation, stabilization or rhizodegradation (pollutant degradation by rhizospheric microorganisms).…”

Section: Introductionmentioning

confidence: 99%

Enhanced remediation of chlorpyrifos from soil using ryegrass (Lollium multiflorum) and chlorpyrifos-degrading bacterium Bacillus pumilus C2A1

Ahmad

Iqbal

Anwar

et al. 2012

Journal of Hazardous Materials

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Section: Introductionmentioning

confidence: 99%

Enhanced remediation of chlorpyrifos from soil using ryegrass (Lollium multiflorum) and chlorpyrifos-degrading bacterium Bacillus pumilus C2A1

Ahmad

Iqbal

Anwar

et al. 2012

Journal of Hazardous Materials

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“…For the environment's decontamination and remediation, an alternative approach, phytodegradation by transgenic plants (e.g. tobacco) expressing a bacterial PTE, has been considered as a potentially low-cost, safe, and effective method (Wang et al, 2008). …”

Section: 2 Potential Enzymesmentioning

confidence: 99%

Catalytic Bioscavengers Against Toxic Esters, an Alternative Approach for Prophylaxis and Treatments of Poisonings

Masson¹,

Rochu²

2009

Acta Naturae

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Bioscavengers are biopharmaceuticals that specifically react with toxicants. Thus, enzymes reacting with poisonous esters can be used as bioscavengers for neutralization of toxic molecules before they reach physiological targets. Parenteral administration of bioscavengers is, therefore, intended for prophylaxis or pre-treatments, emergency and post-exposure treatments of intoxications. These enzymes can also be used for application on skin, mucosa and wounds as active components of topical skin protectants and decontamination solutions. Human butyrylcholinesterase is the first stoichiometric bioscavenger for safe and efficient prophylaxis of organophosphate poisoning. However, huge amounts of a costly enzyme are needed for protection. Thus, the bioscavenger approach will be greatly improved by the use of catalytic bioscavengers. Catalytic bioscavengers are enzymes capable of degrading toxic esters with a turnover. Suitable catalytic bioscavengers are engineered mutants of human enzymes. Efficient mutants of human butyrylcholinesterase have been made that hydrolyze cocaine at a high rate. Mutants of human cholinesterases capable of hydrolyzing OPs have been made, but so far their activity is too low to be of medical interest. Human paraoxonase a promiscuous plasma enzyme is certainly the most promising phosphotriesterase. However, its biotechnology is still in its infancy. Other enzymes and proteins from blood and organs, and secondary biological targets of OPs and carbamates are potential bioscavengers, in particular serum albumin that reacts with OPs and self-reactivates. Lastly, non-human enzymes, phosphotriesterases and oxidases from various bacterial and eukaryotic sources could be used for external use against OP poisoning and for internal use after modifications for immunological compatibility.

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“…A wide range of organisms, including vertebrates, are affected by the organophosphates due to their non-specific mode of action. As a result of their solubility in lipids, these chemicals accumulate in the food chain, thereby increasing their toxicity to humans (Wang et al 2008). Each year, approximately 200,000 fatalities are reported as a consequence of organophosphate poisoning (Karalliedde and Senanayake 1999).…”

Section: Introductionmentioning

confidence: 99%

Detection of the organophosphate degrading gene opdA in the newly isolated bacterial strain Bacillus pumilus W1

et al. 2011

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The toxicity of organophosphates to a wide range of organisms necessitates the study of their degradation. We designed a study to isolate an organophosphate-degrading bacterium and to detect the gene involved in the hydrolysis of organophosphates. The bacterial strain was isolated by the enrichment culture technique from organophosphatecontaminated soil, It was identified as Bacillus pumilus W1 based on its biochemical characteristics and found to be able to grow in the presence of methyl parathion (250 ppm). The organophosphate-degrading gene opdA was detected by colony PCR. The specific activity of the organophosphate-degrading enzyme OpdA was analyzed in a cell extract of the bacterial isolate and found to be 0.34 ±0.14 IU mg -1 protein. The effect of different metals, including Mn 2+ , Mg 2+ , Co 2+ , and Zn 2+ , on the activity of OpdA was also determined; enzyme activity was enhanced by approximately a factor of 4.84±0.39 in the presence of Mn 2+ . Degradation of p-nitrophenol was studied under aerobic conditions, and more than 70% p-nitrophenol was degraded within 24 h in the presence of glucose. This is the first report of an opdA gene in this specific B. pumilus strain.

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Phytodegradation of organophosphorus compounds by transgenic plants expressing a bacterial organophosphorus hydrolase

Cited by 52 publications

References 27 publications

Enhanced remediation of chlorpyrifos from soil using ryegrass (Lollium multiflorum) and chlorpyrifos-degrading bacterium Bacillus pumilus C2A1

Enhanced remediation of chlorpyrifos from soil using ryegrass (Lollium multiflorum) and chlorpyrifos-degrading bacterium Bacillus pumilus C2A1

Catalytic Bioscavengers Against Toxic Esters, an Alternative Approach for Prophylaxis and Treatments of Poisonings

Detection of the organophosphate degrading gene opdA in the newly isolated bacterial strain Bacillus pumilus W1

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