Phytochrome-Mediated Transcriptional Up-regulation of ALLENE OXIDE SYNTHASE in Rice Seedlings

Haga, Ken; Iino, Masashi

doi:10.1093/pcp/pch025

Cited by 79 publications

(67 citation statements)

References 47 publications

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“…AK105964 and AK107161 were previously designated OsAOS4 and OsAOS3 respectively, but the catalytic activities of the recombinant proteins of these cDNAs were not clarified. 25) AK105964 and AK107161 were also annotated as putative AOS on the KOME database, but we thought that they might have a catalytic activity of 9-/13-HPL. To confirm this, we cloned cDNAs corresponding to the longest open reading frame of AK105964 and AK107161 by RT-PCR from polyA þ -RNA extracted from etiolated shoots.…”

Section: Resultsmentioning

confidence: 99%

Identification and Functional Analyses of Two cDNAs That Encode Fatty Acid 9-/13-Hydroperoxide Lyase (CYP74C) in Rice

Kuroda

Oshima

Kaneda

et al. 2005

Bioscience, Biotechnology, and Biochemistry

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Fatty acid hydroperoxide lyase (HPL), a member of cytochrome P450 (CYP74), produces aldehydes and oxoacids involved in plant defensive reactions. In monocots, HPL that cleaves 13-hydroperoxides of fatty acids has been reported, but HPL that cleaves 9-hydroperoxides is still unknown. To find this type of HPL, in silico screening of candidate cDNA clones and subsequent functional analyses of recombinant proteins were performed. We found that AK105964 and AK107161 (Genbank accession numbers), cDNAs previously annotated as allene oxide synthase (AOS) in rice, are distinctively grouped from AOS and 13-HPL. Recombinant proteins of these cDNAs produced in Escherichia. coli cleaved both 9-and 13-hydroperoxide of linoleic and linolenic into aldehydes, while having only a trace level of AOS activity and no divinyl ether synthase activity. Hence we designated AK105964 and AK107161 OsHPL1 and OsHPL2 respectively. They are the first CYP74C family cDNAs to be found in monocots.

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Section: Resultsmentioning

confidence: 99%

Identification and Functional Analyses of Two cDNAs That Encode Fatty Acid 9-/13-Hydroperoxide Lyase (CYP74C) in Rice

Kuroda

Oshima

Kaneda

et al. 2005

Bioscience, Biotechnology, and Biochemistry

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“…A JA-deficient rice mutant, hebiba, also shows male sterility (47). It has been reported that the synthesis of JA is induced by red light in rice and the induction is mediated by phytochromes (48). These lines of evidence suggest the possibility that the regulation of JA synthesis is disrupted in the triple mutant, which leads to impaired dehiscence of anthers.…”

Section: Discussionmentioning

confidence: 99%

Phytochromes are the sole photoreceptors for perceiving red/far-red light in rice

Takano

Inagaki

Xie

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

116

111

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Phytochromes are believed to be solely responsible for red and far-red light perception, but this has never been definitively tested. To directly address this hypothesis, a phytochrome triple mutant (phyAphyBphyC) was generated in rice (Oryza sativa L. cv. Nipponbare) and its responses to red and far-red light were monitored. Since rice only has three phytochrome genes (PHYA, PHYB and PHYC), this mutant is completely lacking any phytochrome. Rice seedlings grown in the dark develop long coleoptiles while undergoing regular circumnutation. The phytochrome triple mutants also show this characteristic skotomorphogenesis, even under continuous red or far-red light. The morphology of the triple mutant seedlings grown under red or far-red light appears completely the same as etiolated seedlings, and they show no expression of the light-induced genes. This is direct evidence demonstrating that phytochromes are the sole photoreceptors for perceiving red and far-red light, at least during rice seedling establishment. Furthermore, the shape of the triple mutant plants was dramatically altered. Most remarkably, triple mutants extend their internodes even during the vegetative growth stage, which is a time during which wild-type rice plants never elongate their internodes. The triple mutants also flowered very early under long day conditions and set very few seeds due to incomplete male sterility. These data indicate that phytochromes play an important role in maximizing photosynthetic abilities during the vegetative growth stage in rice.internodal elongation ͉ mutant ͉ phytochrome ͉ photoreceptor

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“…The selected seedlings were grown further, and the genomic DNA of each plant (1 to 100 mg) was extracted from leaves as described by Haga and Iino (2004). The RFLP, CAPS, and STS markers used to analyze the polymorphisms between Nipponbare and Kasalath were obtained from the DNA bank at the National Institute of Agrobiological Sciences (http://www.dna.affrc.go.jp/misc/bank.index.html).…”

Section: Map-based Cloning Of Cpt1mentioning

confidence: 99%

“…The 39 half of CPT1 cDNA (1.3 kb) was obtained by 39 RACE and cloned into Escherichia coli (strain TOP10) with a GeneRacer kit (the kit with AMV RT, TOPO TA cloning; Invitrogen, Carlsbad, CA) according to the manufacture's instructions. The total RNA (5 mg) used for the 39 RACE was extracted from dark-grown seedling shoots as described by Haga and Iino (2004); the CPT1-specific primer used was 59-CGCTGGTCACCGAGCTCGAGAA-39. The full-length cDNA (2.9 kb) was obtained by fusing the above two clones at the BstEII sites present in the overlapping region.…”

Section: Construction Of Cpt1 Cdnamentioning

confidence: 99%

The Rice COLEOPTILE PHOTOTROPISM1 Gene Encoding an Ortholog of Arabidopsis NPH3 Is Required for Phototropism of Coleoptiles and Lateral Translocation of Auxin

et al. 2005

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We isolated a mutant, named coleoptile phototropism1 (cpt1), from g-ray-mutagenized japonica-type rice (Oryza sativa). This mutant showed no coleoptile phototropism and severely reduced root phototropism after continuous stimulation. A map-based cloning strategy and transgenic complementation test were applied to demonstrate that a NPH3-like gene deleted in the mutant corresponds to CPT1. Phylogenetic analysis of putative CPT1 homologs of rice and related proteins indicated that CPT1 has an orthologous relationship with Arabidopsis thaliana NPH3. These results, along with those for Arabidopsis, demonstrate that NPH3/CPT1 is a key signal transduction component of higher plant phototropism. In an extended study with the cpt1 mutant, it was found that phototropic differential growth is accompanied by a CPT1-independent inhibition of net growth. Kinetic investigation further indicated that a small phototropism occurs in cpt1 coleoptiles. This response, induced only transiently, was thought to be caused by the CPT1-independent growth inhibition. The 3 H-indole-3-acetic acid applied to the coleoptile tip was asymmetrically distributed between the two sides of phototropically responding coleoptiles. However, no asymmetry was induced in cpt1 coleoptiles, indicating that lateral translocation of auxin occurs downstream of CPT1. It is concluded that the CPT1-dependent major phototropism of coleoptiles is achieved by lateral auxin translocation and subsequent growth redistribution.

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Phytochrome-Mediated Transcriptional Up-regulation of ALLENE OXIDE SYNTHASE in Rice Seedlings

Cited by 79 publications

References 47 publications

Identification and Functional Analyses of Two cDNAs That Encode Fatty Acid 9-/13-Hydroperoxide Lyase (CYP74C) in Rice

Identification and Functional Analyses of Two cDNAs That Encode Fatty Acid 9-/13-Hydroperoxide Lyase (CYP74C) in Rice

Phytochromes are the sole photoreceptors for perceiving red/far-red light in rice

The Rice COLEOPTILE PHOTOTROPISM1 Gene Encoding an Ortholog of Arabidopsis NPH3 Is Required for Phototropism of Coleoptiles and Lateral Translocation of Auxin

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