Phytochrome‐mediated closure of <i>Albizzia lophantha</i> leaflets as affected by sorbitol, mannitol and fusicoccin

Moysset, Luisa; Llambrich, Esther; Simón, Esther

doi:10.1111/j.1399-3054.1994.tb00436.x

Cited by 3 publications

(1 citation statement)

References 19 publications

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“…Previous data have shown that in several tissues [carrot cells (Reuveni et al 1987), Arabidopsis thaliana cells (Curti et al 1993), Albizzia lophantha leaflets (Moysset et al 1994), Acer pseudoplatanus cells, pea growing internode segments, and isolated radish cotyledons (P. Lado and L. Zingarelli, unpublished data)] the stimulating effect of osmotic treatment on H + extrusion was additive or synergistic with the stimulating effect of fusicoccin (FC). These findings suggest that the modes of action of osmotica and of FC in stimulating PM H + -ATPase activity differ in at least some step(s).…”

Section: Introductionmentioning

confidence: 99%

Effects of hyper‐osmotic stress on K⁺ fluxes, H⁺ extrusion, transmembrane electric potential difference and comparison with the effects of fusicoccin

Zingarelli

Marré

Massardi

et al. 1999

Physiologia Plantarum

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The stimulation of H+ extrusion by hyper‐osmotic stress (0.2–0.3 M mannitol) in cultured cells of Arabidopsis thaliana (L.) Heynh. was shown to be associated with an inhibition of Cl− efflux, whereas hypo‐osmotic stress, inhibiting H+ extrusion, early and strongly stimulated Cl− efflux. In this paper, we investigate the contribution of other factors [K+ transport and transmembrane electric potential difference (Em)] to the hyper‐osmotic‐induced activation of the plasma membrane (PM) H+‐ATPase. The effects of mannitol (MA) on K+ transport and on Em were compared with those of fusicoccin (FC) since the modes of action of osmotica and of the toxin in stimulating H+‐ATPase activity seem to differ at least in some steps. The changes in H+ extrusion induced by hyper‐ or hypo‐osmotic stress were opposite and could be reversed by the application of the respective opposite stress. The effect of MA on H+ extrusion was dependent on the presence of K+ (or Rb+) similarly to that of FC, while Na+ and Li+, which also stimulated the FC effect, were ineffective on that of MA. The MA effect was independent of the anions (Cl−, SO42−, NO3−) accompanying K+. K+ net uptake and K+ influx were stimulated by both MA and FC. Tetraethylammonium (TEA+) and Cs+ inhibited both MA‐ and FC‐induced H+ extrusion, suggesting the involvement of K+ channels. MA (0.2 M) induced a strong hyperpolarization of Em both in the absence and in the presence of K+. The hyperpolarizing effect of MA was also found when the cells were already hyperpolarized by FC, and was rapidly reversed by removing the osmoticum from the medium. In the presence of the lipophilic cation tributylbenzylammonium (TBBA+), MA was no longer able to stimulate H+ extrusion, while FC still stimulated it. In cells pretreated with TBBA+, which strongly depolarized Em, the subsequent addition of FC repolarized it, while the hyperpolarizing effect of MA was lacking. On the contrary, in cells pretreated with Erythrosine B (EB), Em was strongly depolarized and the following addition of FC did not hyperpolarize it, while the hyperpolarizing effect of MA was still observed. These results suggest that the mechanism of MA in activating H+ extrusion and K+ uptake is different from that of FC. The rise in net K+ uptake seems to be driven by the activation of some hyperpolarizing system that does not seem to depend on a direct activation of PM H+‐ATPase, but rather on the inhibition of Cl− efflux induced by hyper‐osmotic stress.

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Section: Introductionmentioning

confidence: 99%

Effects of hyper‐osmotic stress on K⁺ fluxes, H⁺ extrusion, transmembrane electric potential difference and comparison with the effects of fusicoccin

Zingarelli

Marré

Massardi

et al. 1999

Physiologia Plantarum

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Microautoradiographic localisation of [3H]sucrose and [3H]mannitol in Robinia pseudoacacia pulvinar tissues during phytochrome-mediated nyctinastic closure

et al. 2006

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We have analysed the incorporation of [(3)H]sucrose and [(3)H]mannitol in pulvinar motor cells of Robinia pseudoacacia L. during phytochrome-mediated nyctinastic closure. Pairs of leaflets, excised 2 h after the beginning of the photoperiod, were fed with 50 mM [(3)H]sucrose or [(3)H]mannitol, irradiated with red (15 min) or far-red (5 min) light and placed in the dark for 2-3 h. Label uptake was measured in whole pulvini by liquid scintillation counting. The distribution of labelling in pulvinar sections was assessed by both light and electron microautoradiography. [(3)H]Sucrose uptake was twice that of [(3)H]mannitol incorporation in both red- and far-red-irradiated pulvini. In the autoradiographs, [(3)H]sucrose and [(3)H]mannitol labelling was localised in the area from the vascular bundle to the epidermis, mainly in vacuoles, cytoplasm, and cell walls. Extensor and flexor protoplasts displayed a different distribution of [(3)H]sucrose after red and far-red irradiation. Far-red light drastically reduced the [(3)H]sucrose incorporation in extensor protoplasts and caused a slight increase in internal flexor protoplasts. After red light treatment, no differences in [(3)H]sucrose labelling were found between extensor and flexor protoplasts. Our results indicate a phytochrome control of sucrose distribution in cortical motor cells and seem to rule out the possibility of sucrose acting as an osmoticum.

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Nitric-oxide inhibits nyctinastic closure through cGMP in Albizia lophantha leaflets

Bergareche

Moysset

Angelo

et al. 2014

Journal of Plant Physiology

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Phytochrome‐mediated closure of Albizzia lophantha leaflets as affected by sorbitol, mannitol and fusicoccin

Cited by 3 publications

References 19 publications

Effects of hyper‐osmotic stress on K⁺ fluxes, H⁺ extrusion, transmembrane electric potential difference and comparison with the effects of fusicoccin

Effects of hyper‐osmotic stress on K⁺ fluxes, H⁺ extrusion, transmembrane electric potential difference and comparison with the effects of fusicoccin

Microautoradiographic localisation of [3H]sucrose and [3H]mannitol in Robinia pseudoacacia pulvinar tissues during phytochrome-mediated nyctinastic closure

Nitric-oxide inhibits nyctinastic closure through cGMP in Albizia lophantha leaflets

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Phytochrome‐mediated closure of Albizzia lophantha leaflets as affected by sorbitol, mannitol and fusicoccin

Cited by 3 publications

References 19 publications

Effects of hyper‐osmotic stress on K+ fluxes, H+ extrusion, transmembrane electric potential difference and comparison with the effects of fusicoccin

Effects of hyper‐osmotic stress on K+ fluxes, H+ extrusion, transmembrane electric potential difference and comparison with the effects of fusicoccin

Microautoradiographic localisation of [3H]sucrose and [3H]mannitol in Robinia pseudoacacia pulvinar tissues during phytochrome-mediated nyctinastic closure

Nitric-oxide inhibits nyctinastic closure through cGMP in Albizia lophantha leaflets

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Effects of hyper‐osmotic stress on K⁺ fluxes, H⁺ extrusion, transmembrane electric potential difference and comparison with the effects of fusicoccin

Effects of hyper‐osmotic stress on K⁺ fluxes, H⁺ extrusion, transmembrane electric potential difference and comparison with the effects of fusicoccin