PHYTOCHROME INTERACTING FACTOR3 Associates with the Histone Deacetylase HDA15 in Repression of Chlorophyll Biosynthesis and Photosynthesis in EtiolatedArabidopsisSeedlings  

Abstract

PHYTOCHROME INTERACTING FACTOR3 (PIF3) is a key basic helix-loop-helix transcription factor of Arabidopsis thaliana that negatively regulates light responses, repressing chlorophyll biosynthesis, photosynthesis, and photomorphogenesis in the dark. However, the mechanism for the PIF3-mediated transcription regulation remains largely unknown. In this study, we found that the REDUCED POTASSIUM DEPENDENCY3/HISTONE DEACETYLASE1-type histone deacetylase HDA15 directly interacted with PIF3 in vivo and in vitro. Genome-wide transcriptome analysis revealed that HDA15 acts mainly as a transcriptional repressor and negatively regulates chlorophyll biosynthesis and photosynthesis gene expression in etiolated seedlings. HDA15 and PIF3 cotarget to the genes involved in chlorophyll biosynthesis and photosynthesis in the dark and repress gene expression by decreasing the acetylation levels and RNA Polymerase II-associated transcription. The binding of HDA15 to the target genes depends on the presence of PIF3. In addition, PIF3 and HDA15 are dissociated from the target genes upon exposure to red light. Taken together, our results indicate that PIF3 associates with HDA15 to repress chlorophyll biosynthetic and photosynthetic genes in etiolated seedlings.

PIFs: Systems Integrators in Plant Development

“…Loss of function of the histone deacetylase HDA15 leads to upregulation of a number of chlorophyll biosynthetic and photosynthetic genes accompanied with an increase of histone acetylation in their promoters. The proposed model of how HDA15 regulates light-responsive gene expression involves direct HDA15 binding to PIF3, which was recently shown to recruit HDA15 to the G-box elements of these genes in the dark (Liu et al, 2013b). PIF3 and HDA15 corepress gene expression by decreasing the acetylation levels and RNA polymerase II binding.…”

“…First, although they appear to represent a minority, a significant number of PIFq-bound genes are upregulated in pif mutants especially during the deetiolation response ( Figure 2C; Oh et al, 2009Oh et al, , 2012Zhang et al, 2013), suggesting that PIFs act as transcriptional repressors of these genes. Second, evidence for PIFs acting as intrinsic transcriptional repressors of specific genes has been provided in a number of cases, such as PIF1 repression of PSY expression in the dark (Toledo-Ortiz et al, 2010), PIF7 repression of CBF2 expression under diurnal conditions (Kidokoro et al, 2009;Lee and Thomashow, 2012), and PIF1 and/or PIF3 repression of genes associated with chlorophyll biosynthesis, photosynthesis, and reactive oxygen species (ROS) responses during deetiolation (Chen et al, 2013;Liu et al, 2013b). Collectively, the data are consistent with a model whereby in environments with low or absent phy activation, PIFs accumulate and either activate the expression of PIF-induced genes or inhibit the expression of PIF-repressed genes, and this activity is rapidly reversed by light through phy-induced degradation of the PIFs (Figures 3A and 3B).…”

Transcriptional Dynamics Driving MAMP-Triggered Immunity and Pathogen Effector-Mediated Immunosuppression in Arabidopsis Leaves Following Infection with Pseudomonas syringae pv tomato DC3000

“…The induction of HDA15, encoding a histone deacetylase, which negatively regulates chlorophyll biosynthesis and photosynthesis gene expression in etiolated seedlings (Liu et al, 2013), would be likely to contribute to the sustained suppression of photosynthetic genes observed in Figure 3. Additionally the SET-domain containing genes KRYPTONITE (SDG33) and ASHH1 (SDG26) were induced early in infection.…”

Banana Transcription Factor MaERF11 Recruits Histone Deacetylase MaHDA1 and Represses the Expression of MaACO1 and Expansins during Fruit Ripening

“…An HDAC (histone deacetylase) enzymatic assay was performed according to Liu et al (2013). Soluble total proteins extracted from tobacco leaves transiently expressing 35S:GFP, or 35S:MaHDA1-GFP, were mixed with 25-mL magnetic particles in immunoprecipitation buffer (10 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.5 mM EDTA, and 0.5% [v/v] Nonidet P-40) and incubated overnight at 4°C.…”