PHYTOCHROME-INTERACTING FACTOR-LIKE14 and SLENDER RICE1 Interaction Controls Seedling Growth under Salt Stress

Mo, Weiping; Tang, Weijiang; Du, Yutong; Jing, Yanjun; Bu, Qingyun; Lin, Rongcheng

doi:10.1104/pp.20.00024

Cited by 72 publications

(50 citation statements)

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“…For example, among six OsPILs (OsPIL11 to OsPIL16) ( Nakamura et al, 2007 ), the T-DNA insertion knockdown rice of OsPIL13 exhibited a pale-green phenotype with the downregulation of several genes involved in chlorophyll biosynthesis ( Sakuraba et al, 2017 ), and the CRISPR/Cas9-mediated knockout rice of OsPIL15 increased the number of cells in the grains ( Ji et al, 2019 ). Furthermore, rice and maize plants overexpressing PIFs exhibited phenotypes related to seedling growth, internode elongation, and chlorophyll biosynthesis ( Todaka et al, 2012 ; Zhou et al, 2014 ; Sakuraba et al, 2017 ; Shi et al, 2017 ; Mo et al, 2020 ). Here, we also reported similar phenotypes, but along with the positive regulatory roles of BdPIL1 and BdPIL3 in floral induction, which might be correlated with the expression of both BdPIL1 and BdPIL3 in the flower tissues ( Figure 1E ).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the plants of both RNAi lines exhibit increased expression of BdSAUR and BdGA20ox2 genes ( Figures 5E,F ), which might correlate with the tall phenotype, because these genes play roles in promoting elongated growth ( Rieu et al, 2008 ; Stortenbeker and Bemer, 2019 ). Previously, OsPIL13 and OsPIL14 have been shown to bind to the promoters of the genes, such as OsPORB ( Sakuraba et al, 2017 ; Mo et al, 2020 ). Thus, we analyzed the DNA-binding ability of BdPIL1 and BdPIL3 with the promoter sequences of selected target genes, namely, BdPOR , BdMIR156H , and BdSAUR50 , and suggested that BdPIL1 and BdPIL3 regulated the transcription of target genes by directly binding to the corresponding promoters ( Figure 7 and Supplementary Figures 12 , 13 ).…”

Section: Discussionmentioning

confidence: 99%

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Suppression of Phytochrome-Interacting Factors Enhances Photoresponses of Seedlings and Delays Flowering With Increased Plant Height in Brachypodium distachyon

et al. 2021

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Phytochromes are red and far-red photoreceptors that regulate plant growth and development under ambient light conditions. During phytochrome-mediated photomorphogenesis, phytochrome-interacting factors (PIFs) are the most important signaling partners that regulate the expression of light-responsive genes. However, the function of PIFs in monocots has not been studied well. In this study, using RNA interference (RNAi), we investigated the functions of BdPIL1 and BdPIL3, two PIF-like genes identified in Brachypodium distachyon, which are closely related to Arabidopsis PIF1 and PIF3. The expression of their genes is light-inducible, and both BdPIL1 and BdPIL3 proteins interact with phytochromes in an active form-specific manner. Transgenic Brachypodium seedlings with the RNAi constructs of BdPIL1 and BdPIL3 showed decreased coleoptile lengths and increased leaf growth when exposed to both red and far-red light. In addition, the transgenic plants were taller with elongated internodes than wild-type Bd21-3 plant, exhibiting late flowering. Moreover, RNA-seq analysis revealed downregulation of many genes in the transgenic plants, especially those related to the regulation of cell number, floral induction, and chlorophyll biosynthesis, which were consistent with the phenotypes of increased plant height, delayed flowering, and pale green leaves. Furthermore, we demonstrated the DNA-binding ability of BdPIL1 and BdPIL3 to the putative target promoters and that the DNA-binding was inhibited in the presence of phytochromes. Therefore, this study determines a molecular mechanism underlying phytochrome-mediated PIF regulation in Brachypodium, i.e., sequestration, and also elucidates the functions of BdPIL1 and BdPIL3 in the growth and development of the monocot plant.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Suppression of Phytochrome-Interacting Factors Enhances Photoresponses of Seedlings and Delays Flowering With Increased Plant Height in Brachypodium distachyon

et al. 2021

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“…The expression of OsAGO18 can be activated by jasmonate (JA) signalling [ 40 ]. OsSLRL1 is a DELLA family protein that regulates plant hormone metabolism and mediates the growth and development of plants [ 41 – 43 ]. qRT-PCR was performed and the relative expression levels of the selected candidate genes were determined in plants infected by the virus from 0 to 16 days.…”

Section: Resultsmentioning

confidence: 99%

The dynamics of N6-methyladenine RNA modification in interactions between rice and plant viruses

et al. 2021

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Background N6-methyladenosine (m6A) is the most common RNA modification in eukaryotes and has been implicated as a novel epigenetic marker that is involved in various biological processes. The pattern and functional dissection of m6A in the regulation of several major human viral diseases have already been reported. However, the patterns and functions of m6A distribution in plant disease bursting remain largely unknown. Results We analyse the high-quality m6A methylomes in rice plants infected with two devastating viruses. We find that the m6A methylation is mainly associated with genes that are not actively expressed in virus-infected rice plants. We also detect different m6A peak distributions on the same gene, which may contribute to different antiviral modes between rice stripe virus or rice black-stripe dwarf virus infection. Interestingly, we observe increased levels of m6A methylation in rice plant response to virus infection. Several antiviral pathway-related genes, such as RNA silencing-, resistance-, and fundamental antiviral phytohormone metabolic-related genes, are also m6A methylated. The level of m6A methylation is tightly associated with its relative expression levels. Conclusions We revealed the dynamics of m6A modification during the interaction between rice and viruses, which may act as a main regulatory strategy in gene expression. Our investigations highlight the significance of m6A modifications in interactions between plant and viruses, especially in regulating the expression of genes involved in key pathways.

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“…Salinity is another major abiotic stress that is globally distributed in both irrigated and non-irrigated areas [16,17]. On a global basis, salinity stress ranked second after the drought [18].…”

Section: Introductionmentioning

confidence: 99%

“…As NUE for rice plants under salt-affected soils is relatively lower than those on normal soils, the judicious use of nitrogenous fertilizer application in saline soil is needed. Breeders involved in salinity tolerant rice, it is groundbreaking news that the overexpression of PHYTOCHROME-INTERACTING FACTOR-LIKE14 (OsPIL14), or loss of function of the DELLA protein SLENDER RICE1 (SLR1), accelerate mesocotyl and root growth under salt stress and minimize the sensitivity to NaCl-induced hindrance of seedling growth in rice [17].…”

Section: Introductionmentioning

confidence: 99%

Nitrogen Use Efficiency in Rice under Abiotic Stress: Plant Breeding Approach

Mondal¹,

Hasan²,

Biswas³

et al. 2021

Recent Advances in Rice Research

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Nitrogenous fertilizer has remarkably improved rice (Oryza sativa L.) yield across the world since its discovery by Haber-Bosch process. Due to climate change, future rice production will likely experience a wide range of environmental plasticity. Nitrogen use efficiency (NUE) is an important trait to confer adaptability across various abiotic stresses such as flooding, drought and salinity. The problem with the increased N application often leads to a reduction in NUE. New solutions are needed to simultaneously increase yield and maximize the NUE of rice. Despite the differences among flooding, salinity and drought, these three abiotic stresses lead to similar responses in rice plants. To develop abiotic stress tolerant rice varieties, speed breeding seems a plausible novel approach. Approximately 22 single quantitative trait loci (QTLs) and 58 pairs of epistatic QTLs are known to be closely associated with NUE in rice. The QTLs/genes for submergence (SUB1A) tolerance, anaerobic germination (AG, TPP7) potential and deepwater flooding tolerance (SK1, SK2) are identified. Furthermore, phytochrome-interacting factor-like14 (OsPIL14), or loss of function of the slender rice1 (SLR1) genes enhance salinity tolerance in rice seedlings. This review updates our understanding of the molecular mechanisms of abiotic stress tolerance and discusses possible approaches for developing N-efficient rice variety.

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PHYTOCHROME-INTERACTING FACTOR-LIKE14 and SLENDER RICE1 Interaction Controls Seedling Growth under Salt Stress

Cited by 72 publications

References 48 publications

Suppression of Phytochrome-Interacting Factors Enhances Photoresponses of Seedlings and Delays Flowering With Increased Plant Height in Brachypodium distachyon

Suppression of Phytochrome-Interacting Factors Enhances Photoresponses of Seedlings and Delays Flowering With Increased Plant Height in Brachypodium distachyon

The dynamics of N6-methyladenine RNA modification in interactions between rice and plant viruses

Nitrogen Use Efficiency in Rice under Abiotic Stress: Plant Breeding Approach

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