Phytochemicals as Anticancer Drugs: Targeting the Microtubular Network of Cancer Cells

Khan, Rajni; Chatterjee, Aroni; Ghosh, Hiya; Kapoor, Sonia; Ray, Shashwati

doi:10.1007/978-981-13-6920-9_3

Cited by 2 publications

(1 citation statement)

References 42 publications

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“…Microtubule-targeting agents are split into two classes based on how they affect microtubules: the microtubule-polymerizing or -stabilizing agents and the microtubule-depolymerizing agents [ 15 ]. Paclitaxel, docetaxel, and epothilones induce the polymerization of microtubules, while colchicine, vinca alkaloids, dolastatin, combretastatin, and 2-methoxyestradiol are known to depolymerize microtubules [ 16 ]. By activating the spindle assembly checkpoint, a delay in chromosome congression prevents the metaphase-to-anaphase transition and arrests cells at mitosis [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Avermectin B1a Shows Potential Anti-Proliferative and Anticancer Effects in HCT-116 Cells via Enhancing the Stability of Microtubules

Hoti

Rüstem

Dalmızrak

2023

CIMB

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Avermectins are a group of macrocyclic lactones that are commonly used as pesticides to treat pests and parasitic worms. Some members of the avermectin family, such as ivermectin, have been found to exhibit anti-proliferative activity toward cancer cells. This study aimed to investigate the potential anti-cancer activities of avermectin B1a using the HCT-116 colon cancer cell line. The MTT assay was used to calculate the IC50 by incubating cells with increasing doses of avermectin B1a for 24, 48, and 72 h. Flow cytometry was used to evaluate apoptosis following the 24 h incubation of cells. The migration capacity of the HCT-116 cells in the absence or presence of avermectin B1a was also investigated. Finally, tubulin polymerization in the presence of avermectin B1a was evaluated. Avermectin B1a presented anti-proliferative activity with an IC50 value of 30 μM. Avermectin B1a was found to promote tubulin polymerization at 30 μM. In addition, avermectin B1a induced apoptosis in HCT-116 cells and substantially diminished their ability to migrate. Avermectin B1a exhibits significant anti-cancer activity and enhances tubulin polymerization, suggesting that it can be used as a promising microtubule-targeting agent for the development of future anticancer drugs.

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Section: Introductionmentioning

confidence: 99%

Avermectin B1a Shows Potential Anti-Proliferative and Anticancer Effects in HCT-116 Cells via Enhancing the Stability of Microtubules

Hoti

Rüstem

Dalmızrak

2023

CIMB

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Anticancer Potential of Calli Versus Seedling Extracts Derived from Rosmarinus officinalis and Coleus hybridus

Albogami

Darwish

Abdelmigid

et al. 2020

CPB

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Background: In Saudi Arabia, the incidence and mortality rates of breast cancer are high. Although current treatments have proven their efficacy, breast cancer cells can gain resistance against these treatments, leading to multifaceted complications. Numerous studies have demonstrated that plant phytochemicals and active compounds can counteract cancer. Rosmarinic acid (RA), a phenolic compound abundant in the family Lamiaceae, has been shown to have anticancer properties. Objective: The aim of this study was to investigate the anticancer properties of methanolic crude extracts of seedlings and calli of Coleus hybridus and Rosmarinus officinalis, two Lamiaceae species. Methods: MCF-7 human breast cancer cells wear treated with RA-rich methanolic crude extracts obtained from plant calli and seedlings generated in vitro, and we subjected the cells to proliferation assays. Transcriptional profiling of the seedling and callus tissues was also conducted. Results: mRNA expression levels of RA genes were higher in C. hybridus seedlings than in R. officinalis seedlings, and, except for TAT and C4H, in C. hybridus calli than in R. officinalis calli. These findings were in agreement with the anti-proliferative effects of seedling and callus extracts of both R. officinalis and C. hybridus in MCF-7 cells after 24 or 48 h of exposure. C. hybridus callus extracts with RA concentrations of 100 µg/mL and 200 µg/mL showed the most significant anti-proliferative effect after 48 h of exposure (p < 0.001) when compared to R. officinalis callus extracts. Similarly, C. hybridus seedling extracts with RA concentrations of 90 µg/mL and 150 µg/mL exhibited significant anti-proliferative effects after 48 h of exposure (p < 0.001). R. officinalis seedling extract with 150 µg RA/ml also had a significant effect after 48 h of treatment (p < 0.001). Conclusion: Methanolic extracts of C. hybridus and R. officinalis provide a potentially valuable option for breast cancer treatment.

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Phytochemicals as Anticancer Drugs: Targeting the Microtubular Network of Cancer Cells

Cited by 2 publications

References 42 publications

Avermectin B1a Shows Potential Anti-Proliferative and Anticancer Effects in HCT-116 Cells via Enhancing the Stability of Microtubules

Avermectin B1a Shows Potential Anti-Proliferative and Anticancer Effects in HCT-116 Cells via Enhancing the Stability of Microtubules

Anticancer Potential of Calli Versus Seedling Extracts Derived from Rosmarinus officinalis and Coleus hybridus

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